关键词: 南江黄羊, LHβ基因, 核苷酸序列, 氨基酸, 系统发育分析

Abstract: According to the LHβ gene sequence of cow, sheep and pig in GenBank, the primers were designed and partial sequence of LHβ gene from 35 Nanjiang Huang goat blood samples were amplified and sequenced by PCR method (GenBank No:AY853264). Furthermore, phylogenetic tree of different species were conducted using the LHβ partial coding sequence. The result showed that the 929bp partial sequence of LHβ gene included 5′flanking region (136bp), two introns (297bp and 235bp), two exons (26bp and 168bp) and partial sequence of exon3 (67bp). Exon sequence coded 83 amino acids except the 11bp nucleotide at the beginning of 1st exon ,which was the 5′untranslating region(5′UTR), while the first 20 amino acids were the signal peptide. LHβ gene was rich with GC. Eight nucleotide mutations were detected and two of them existed in exon were synonymous mutation. Phylogenetic relationship was not in accord with the actual species evolution process, which maybe caused by the different GC proportion of LHβ gene among species. It was the first time to report goat LHβ gene sequence which established the foundation of the further research about genetic principle of goat reproduction.

Key words: Nanjiang Huang goat, LHβ gene, nucleotide sequence, amino acid, phylogenetic analysis