关键词: 鸡与鹌鹑, 属间杂交, 杂交不育, mtDNA-RFLP, 母性遗传

Abstract: The hybrid experiment has been proceeded between chicken and quail through the artificial insemination. The hybrids of layer(♂)—quail(♀), blackbone chicken(♂)—quail(♀) were gained successfully .The experiment indicated that the hybrids expressed strongly crossing sterility—early female embryo death，that is to say the differentiations of early cell stopped in female embryo. In order to study the genetic rule of cytoplasm in distance hybridization，the isolation of mitochondrial DNA from different viscera were largely founded in chicken,black—bone chicken,quail and their hybrids.The results showed that the intranuclear genome of hybrid and female parent appeared highly uniformity on mtDNA size, but different from their male. This paper analysized nine restriction endonuclease were applied to restriction fragment in mtDNA of chicken, blackbone chicken, quail and hybrids. The results indicated the hybrids have the same mtDNA haplotypes as their parent. This approved that maternal inheritance of mtDNA exist in chicken—quail interliner hybridization.

Key words: chicken—quail, interliner hybridization, crossing sterility, mtDNA RFLP, maternal inheritance