关键词: 斯氏副柔线虫, 内转录间隔区, PCR, 克隆, 序列分析

Abstract: The internal transcribed spacer （ITS1, 5.8S and ITS2）of ribosomal DNA nucleotide fragments of Parabronema skrjabini isolated from the camel of Inner Mongolia region were amplified by PCR using three pairs of conserved primers NC5, NC13, NC13r and NC2. The PCR fragments were purified and cloned into pGM-T vector. The inserts were successfully sequenced, and the results revealed that the first sample′s (P.sk1) ITS inserts were 837 bp in length and consisted of partial 18S, 28S and complete ITS1（298 bp）, 5.8S（157 bp） and ITS2 （281）rDNA sequences. The second sample’s (P.sk2) ITS1 inserts were 372 bp in length and consisted of partial 18S, 5.8S and complete ITS1（296 bp）. The third sample’s (P.sk3) ITS2 inserts were 484 bp in length and consisted of partial 5.8S, 28S and complete ITS2（284 bp）. ITS2 sequences shared 30.2%-60.1% homology with other nematodes. It was the first time that the complete sequence of ITS and 5.8S rDNA of P. skrjabini were reported. The results of this study laid a foundation for further studies.

Key words: Parabronema skrjabini, internal transcribed spacer, PCR, cloning, sequence analysis