Ghrelin免疫阳性细胞在山羊胃肠道的发育性变化

畜牧兽医学报 ›› 2012, Vol. 43 ›› Issue (7): 1042-1048.

Ghrelin免疫阳性细胞在山羊胃肠道的发育性变化

张德敏1,2，王树迎1,2*，尹玉涛1,2，刘宵1,2，侯衍猛1,2，黄丽波1,2，张春兰3

1.山东农业大学动物科技学院，泰安 271018； 2. 山东农业大学动物科技学院动物生物技术与疾病防治重点实验室，泰安 271018； 3. 山东省牧工商总公司，济南 250022

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-07-27 发布日期:2012-07-27
通讯作者: 王树迎

Developmental Changes of Ghrelinimmunopositive Cells in the Gastrointestinal Tract of Goat

ZHANG Demin1,2, WANG Shuying1，2*, YIN Yutao1,2, LIU Xiao1,2,

HOU Yanmeng1,2, HUANG Libo1,2, ZHANG Chunlan3

1. College of Animal Science and Technology, Shandong Agricultural University, Tai’an 271018, China; 2. Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, Tai’an 271018, China; 3. Shandong Animal Husbandry Group, Jinan 250022, China

Received:1900-01-01 Revised:1900-01-01 Online:2012-07-27 Published:2012-07-27
Contact: WANG Shuying1

摘要/Abstract

摘要：

为探究Ghrelin免疫阳性细胞山羊胃肠道的定位分布、形态特征与发育性变化，选用0、30、60、90、120、150和180日龄雌性济宁青山羊，按试验要求取材后进行免疫组织化学方法染色。结果显示，Ghrelin免疫阳性细胞主要分布在黏膜上皮、腺体和固有层，有“闭合型”和“开放型”2种类型。2种类型细胞在皱胃、十二指肠、空肠、回肠、盲肠和结肠均有分布，但未在瘤胃、网胃、瓣胃和直肠中检出。Ghrelin阳性细胞在皱胃中最多，由皱胃至结肠逐渐减少，“闭合型”细胞所占比例也由皱胃至结肠逐步减少。从0～120日龄，皱胃和空肠中阳性细胞数随着年龄增长而增加，150和180日龄有所下降；十二指肠中阳性细胞在90日龄达到高峰。回肠、盲肠和结肠中阳性细胞数较少，均呈现随日龄增长而缓慢增长的趋势。Ghrelin免疫阳性细胞的数量变化表明Ghrelin可能与山羊胃肠道发育密切相关。

关键词: Ghrelin, 济宁青山羊, 胃肠道, 免疫组化

Abstract:

In the present study, the distribution, morphological characteristics and developmental changes of Ghrelinimmunopositive (Ghrelinip) cells in the gastrointestinal tract of goat were investigated. Using the method of immunohistochemistry, Ghrelinip cells were showed in the gastrointestinal tract of Jining Gray goat at the ages of day 0,30,60,90,120,150 and 180. Ghrelinip cells classified into open and closedtype cells were found to be localized in the mucosa epithelium, glands and lamina propria of abomasums, duodenum, jejunum, ileum, cecum and colon, but not in rumen, reticulum, omasum and rectum. The greatest number of Ghrelinip cells was found in the abomasums and the Ghrelinip cell density gradually decreased from the abomasums to the colon. The percentage of closedtype cells to all immunopositive cells also decreased from the abomasums to the colon. With the goat growth, the densities of Ghrelinip cells in the abomasums and jejunum increased from ages of day 0 to 120, but slightly decreased at ages of day 150 and 180. There was also a steady increase in the number of Ghrelinip cells from postnatal day 0 to 90 in duodenum, which reached a peak and remained steady. Very few Ghrelinip cells were found in the ileum, cecum and colon, and the densities increased with the age growth. The change of Ghrelinimmunopositive cells in the gastrointestinal tract of growing goats suggests that Ghrelin may be involved in gastrointestinal tract development.

Key words: Ghrelin, Jining Gray goat, gastrointestinal tract, immunohistochemistry

张德敏;王树迎;尹玉涛;刘宵;侯衍猛;黄丽波;张春兰. Ghrelin免疫阳性细胞在山羊胃肠道的发育性变化[J]. 畜牧兽医学报, 2012, 43(7): 1042-1048.

ZHANG Demin;WANG Shuying;YIN Yutao;LIU Xiao; HOU Yanmeng;HUANG Libo;ZHANG Chunlan. Developmental Changes of Ghrelinimmunopositive Cells in the Gastrointestinal Tract of Goat[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(7): 1042-1048.

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