关键词: 糖尿病, 链脲佐菌素, 丹参, 血糖, 脾, 肾

Abstract: To study the effect of Danshen Root on the glucose, the structure of spleen and kidney in diabetic rats induced by STZ. And the diabetes model was established by Streptozotocin injected 60 mg·kg^-1. The SD rats were randomly divided into two groups as diabetes group and Danshen treated group, 15 in each group.There were 20 rats in Normal control group. Danshen treated group were orally administered by Danshen Root boiled liquid (5 g·kg^-1·d^-1)， and the diabetes group， normal control group were administered by the similar dosage of distilled water The period of experiment was 28 days. At the 7th, 14th, 21th and 28th day, glucose was measured in serum that is prepared by taking blood through orbital venous plexus and centrifuging. At the end of the experiment, histological section of all rats′ spleen and kidney were made and observed after Bouin liquid fixing and HE staining. Glucose of Danshen treated group were significantly lower than that of the model control group (P<0.01) and higher than that of the normal control group (P<0.01). For the model control group, the following results of histological observation showed that there were less splenic cords, invisible germinal centers and thinner periarterial lymphatic sheath, larger renal glomerulus, smaller renal capsule, protein exudates and exfoliative epithelial cells in renal tubule. For the Danshen treated group, the observation results showed that splenic cords with clear demarcation, visible germinal centers and thicker periarterial lymphatic sheath, closely aligned lymphocytes, normally aligned renal glomerulus, renal capsule and renal tubule, normal epithelial cells but a small quantity of homogeneously redstained protein exudates. The results showed that Danshen Root could improve the abnormal glycometabolism of diabetic rats and help prevent the histological change of the spleen and kidney for diabetes model rats.

Key words: diabetic mellitus, STZ, Danshen Root, glucose, spleen, kidney