关键词: 鸡, GNRHR基因, IGF-1基因, 繁殖, SNP

Abstract: Gonadotropinreleasing hormone receptor (GNRHR) gene and insulinlike growh factor 1 (IGF-1) gene which control the reproductive traits were studied as candidate genes on the Wenchang chicken（Chinese indigenous breed）in the present study. The single nucleotide polymorphism of GNRHR and IGF-1 was detected for the association with the total egg production (NE)，average days of continual egglaying (ADCE) and number of doubleyolked eggs (DYE) in Wenchang chicken. PCRRFLP method was used for identification of genotypes. The frequency of restriction enzyme A/a alleles in the population was for GNRHR 0.69 (Bpu1102I A) and 0.31 (Bpu1102I a) and for IGF1 0.53 (PstI B) and 0.47 (PstI b). χ2 analysis of the two genes indicated that the frequencies of GNRHR、IGF-1 fit HardyWeinberg equilibrium. The result showed that GNRHR Bpu1102I Aa genotype had greater number of eggs (300d) at 87.91 compared to 81.20 for Bpu1102I AA (P<0.01). The GNRHR Bpu1102I Aa genotype had greater number of eggs (400 d) at 13547 compared to 125.47 for Bpu1102I AA (P<001). The IGF-1 PstI bb genotype had greater number of eggs (300 d) at 89.03 compared to 82.61 for PstⅠ BB (P<0.05) and PstⅠ bb genotype also had greater number of eggs (400 d) at 137.84 compared to 127.82 for PstⅠ BB (P<0.05). The IGF-1 PstⅠ bb genotype had greater ADCE at 3.38 compared to 2.96、2.78 for PstⅠ BB and Bb (P<0.05) .Two significant effects of genes’ marker were found: GNRHR of dominant effect on number of eggs and IGF-1 of additive effect on that. The current research supports the effects of GNRHR and IGF-1 genes on reproductive traits of chickens.

Key words: chicken, GNRHR, IGF1, reproduction, SNP