关键词: c-jun, 癌基因, 肉鸡腹水综合征, 高盐

Abstract: In this study, the expression of c-jun mRNA in the wall of pulmonary arterioles was investigated to clarify its possible effect during the development of AS induced by excess salt in broiler chickens. One hundred 8 days old male Arbor Acres broilers were randomly divided into control group(C) and treated group (T). Birds in the treated group were supplied with drinking water added 0.30% NaCl, and other factors were same as that in control group. Lung samples of 6 randomly selected birds per group were collected at 15, 22,29,36,43 and 50 days old respectively to make routine paraffin section. Expression of c-jun mRNA was determined by in situ hybridization(ISH) and image analysis program. The results showed that there was dynamic expression of c-jun mRNA in the wall of pulmonary arterioles of broiler chickens. c-jun mRNA was expressed mostly at 22 days old in the control group. The relative content of c-jun mRNA in the wall of all pulmonary arterioles in the treated group was significantly higher than that of control group at 15, 29 and 36 days old (P<0.01), but at 43 and 50 days old significant difference were found only in outer diameter 20-50 μm pulmonary arterioles wall (P<0.01). These results suggested that excess salt accelerated expression of c-jun mRNA particularly in outer diameter 20-50 μm pulmonary arterioles wall，and that expression of c-jun mRNA may play an important role during the development of AS.

Key words: c-jun, oncogene, broiler ascites syndrome, excess salt