关键词: Ghrelin, 皖西白鹅, 小肠, 免疫组化

Abstract: Objective To study the distribution pattern of ghrelin-producing cells in small intestine of WanXi white geese. Methods The immunohistochemical SABC method and DAB staining technology were used. Results Ghrelin immunopositive cells (ghrelin-ic) were mainly found in the mucosal layer of the small intestine, but not in the submucosa and tunica muscularis. These positive cells, the intensely immunoreactive stain was found in the basal cytoplasm, named ‘‘closed type cells’’, which were rounded or oblong in shape, were not in contact with the lumen. A few ghrelin-ic, which were cone in shape, showed an apical process that contacted the lumen and were the so-called ‘‘open-type cells’’. In the duodenum in particular the two types of ghrelin-ic, ‘‘closed’’ and ‘‘open’’cells, were still observed along villi intestinales. Ghrelin-ic were less numerous in the jejunum and ileum, and were generally of the ‘‘closed’’ type. Conclusion The results suggested that ghrelin-producing cells exist in the mucosal layer of the small intestine of the adult WanXi white goose, ghrelin may regulate the function of the small intestine.

Key words: WanXi white goose, small intestine, ghrelin, immunohistochemistry