牛肝中阿维菌素类药物残留的高效液相色谱荧光检测方法的研究

畜牧兽医学报 ›› 2006, Vol. 37 ›› Issue (5): 500-503.

牛肝中阿维菌素类药物残留的高效液相色谱荧光检测方法的研究

侯晓林;何继红;杜向党;沈建忠

1.中国农业大学动物医学院药理与毒理学系，北京 100094;2.中牧股份有限公司，北京 100070；3.河南农业大学牧医学院药理与毒理学系，郑州 450002

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-05-25 发布日期:2006-05-25

Determination of Residual Avermectins in the Cattle Liver Using HPLC with Fluorescence Detection

HOU Xiao-lin;HE Ji-hong; DU Xiang-dang;SHEN Jian-zhong

1.Department of Pharmacology and Toxicology, College of Veterinary Medicine,China Agricultural University, Beijing 100094, China;2. China Animal Husbandry Industry Co. Lid., Beijing 100070,China;3.Department of Pharmacology and Toxicology, College of Husbandry and Veterinary, Henan Agricultural University, Zhengzhou 450002,China

Received:1900-01-01 Revised:1900-01-01 Online:2006-05-25 Published:2006-05-25

摘要/Abstract

摘要： 采用高效液相色谱分离，荧光检测器检测，建立了固相萃取分析牛组织中埃普利诺菌素、阿维菌素、多拉菌素和伊维菌素残留的方法。样品用乙腈提取，碱性氧化铝和C₁₈SPE柱净化，用乙酸酐和1-甲基咪唑的乙腈溶液作衍生化试剂，在96 ℃条件下，完全衍生化需要100 min。4种药物的平均回收率为70.02%~88.75%，日内变异系数小于8.52%，日间变异系数小于7.13%。埃普利诺菌素、阿维菌素、多拉菌素和伊维菌素的检出限为0.4~0.5 μg/kg，定量限为2 μg/kg。该方法可靠、灵敏，可用于常规残留分析。

关键词: 埃普利诺菌素, 阿维菌素, 多拉菌素, 伊维菌素, 测定, 牛肝脏

Abstract: A method using high-performance liquid chromatography (HPLC) with fluorescence detection was presented for the simultaneous measurement of the eprinomectin, avermectin, doramectin and ivermectin in liver. Samples were extracted with acetonitrile and cleaned up using solid phase extraction on alumina-B and C₁₈ column, and followed by derivatization with acetic anhydride and 1-methylimidazole in acetonitrile. The optimum time required for the completion of derivation reaction of the 4 drugs was 100 min at 96℃. Mean recoveries of the 4 drugs in bovine liver ranged from 70.02% to 88.75%. The intra-and inter-day variations were less than 8.52% and 7.13% respectively. The limit of detection was 0.4-0.5 μg/kg, and the limit of quantitation of the method was 2 μg/kg for all of the 4 drugs. The procedure provides a reliable and sensitive method for routine residual analysis.

Key words: eprinomectin, avermectin, doramectin, ivermectin, determination, cattle liver

侯晓林;何继红;杜向党;沈建忠. 牛肝中阿维菌素类药物残留的高效液相色谱荧光检测方法的研究[J]. 畜牧兽医学报, 2006, 37(5): 500-503.

HOU Xiao-lin;HE Ji-hong;DU Xiang-dang;SHEN Jian-zhong. Determination of Residual Avermectins in the Cattle Liver Using HPLC with Fluorescence Detection[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2006, 37(5): 500-503.

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