畜牧兽医学报 ›› 2012, Vol. 43 ›› Issue (6): 849-856.doi:

• 遗传繁育 • 上一篇    下一篇

VLDLR基因多态性与蛋黄性状的关联分析

曹顶国1,2,周艳1,2,雷秋霞1,2,韩海霞1,2,李福伟1,2,李桂明1,2,盛倩1,3,王存芳3,逯岩1,2*   

  1. 1. 山东省农业科学院家禽研究所,济南 250023; 2. 山东省家禽育种工程技术研究中心,济南 250023; 3. 山东轻工业学院食品与生物工程学院,济南 250353
  • 收稿日期:2011-10-11 修回日期:1900-01-01 出版日期:2012-06-25 发布日期:2012-06-25
  • 通讯作者: 逯岩
  • 作者简介:曹顶国(1973-),男,山东嘉祥人,研究员,博士生,主要从事家禽育种研究,E-mail:jqsyzs@163.com
  • 基金资助:

    山东省良种工程重大项目(2008lz015)

Association of Polymorphisms of VLDLR Gene with Yolk Traits in Chicken

CAO Ding-guo1,2, ZHOU Yan1,2, LEI Qiu-xia1,2, HAN Hai-xia1,2, LI Fu-wei1,2, LI Gui-ming1,2, SHENG Qian1,3, WANG Cun-fang3, LU Yan1,2*   

  1. 1. Institute of Poultry Science, Academy of Agricultural Sciences of Shandong Province, Jinan 250023, China; 2. Poultry Breeding Engineering Technology Center of Shandong Province,Jinan 250023, China; 3. School of Food and Bioengineering, Shandong Polytechnic University, Jinan 250353, China
  • Received:2011-10-11 Revised:1900-01-01 Online:2012-06-25 Published:2012-06-25
  • Contact: LU Yan

摘要:

本研究采用PCR-RFLP方法,检测VLDLR基因在山东地方鸡种汶上芦花鸡、济宁百日鸡和莱芜黑鸡中的多态性,并分析各多态位点不同基因型与4个蛋黄性状的相关性。结果表明,在3个供试群体中共检测到2个具有生物学意义的SNPs位点,即第6外显子8 467 nt 处的G→A突变(V8467)和第17内含子13 876 nt 处的A→G突变(V13876)。最小二乘分析结果表明,V8467对蛋黄质量有极显著影响(P<0.01),对蛋黄高度有显著影响(P<0.05);V13876对蛋黄质量有极显著影响(P<0.01),对蛋黄比率和蛋黄高度有显著影响(P<0.05);而双倍型对蛋黄质量、蛋黄比率的影响均达到极显著水平(P<0.01),对蛋黄高度的影响达到显著水平(P<0.05)。结果提示:本研究检测到的VLDLR基因的V8467和V13876多态性位点可以作为蛋黄性状的候选分子标记。

Abstract:

The SNPs of VLDLR gene in Wenshang Luhua chicken, Jining Bairi chicken and Laiwu Black chicken were detected by PCR-RFLP method. And the correlation between the polymorphism loci and the yolk traits were analyzed. As a result, a G/A mutation at nt 8 467 in VLDLR-exon6 and an A/G mutation at nt 13 876 in VLDLR-intron17 were identified. The least square analysis showed that the V8467 was significantly associated with yolk weight (P<0.01) and yolk height (P<0.05); the V13876 was significantly associated with yolk weight (P<0.01), yolk rate (P<0.05) and yolk height (P<0.05); the diplotype was significantly associated with yolk weight (P<0.01), yolk rate (P<0.01) and yolk height (P<0.05). These results indicated that V8467 and V13876 loci of VLDLR gene would be useful candidate markers in selection program on yolk traits.

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