鼠李糖乳杆菌对Caco2细胞抗氧化功能和细胞因子分泌的影响

畜牧兽医学报 ›› 2012, Vol. 43 ›› Issue (2): 250-254.doi:

鼠李糖乳杆菌对Caco2细胞抗氧化功能和细胞因子分泌的影响

黄怡1，2，黄琴1，李卫芬1*，余东游1，周绪霞1

1. 浙江大学动物科学学院动物分子营养学教育部重点实验室，杭州 310058；2. 广西大学动物科学技术学院，南宁 530005

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-02-15 发布日期:2012-02-15
通讯作者: 李卫芬

Effects of Lactobacillus rhamnosus on Antioxidative Functions and Cytokines Secretion in Caco2 Cells

HUANG Yi1,2, HUANG Qin1, LI Weifen1*, YU Dongyou1, ZHOU Xuxia1

1. Key Laboratory of Animal Molecular Nutrition of Ministry of Education, Colleg of Animal Science, Zhejiang University, Hangzhou 310058, China; 2.College of Animal Science and Technology， Guangxi University, Nanning 530005, China

Received:1900-01-01 Revised:1900-01-01 Online:2012-02-15 Published:2012-02-15

摘要/Abstract

摘要： 本研究旨在探明鼠李糖乳杆菌（L. rhamnosus）对Caco2细胞抗氧化和非特异免疫功能的影响。Caco2细胞分别用PBS（A组）、E. coli K88（B组）和L. rhamnosus（C组）处理，以及先用L. rhamnosus预处理，再用E. coli K88处理（D组）。结果表明，B组细胞上清的TAOC浓度显著降低（P＜0.01），SOD活力显著提高（P＜0.01），C组与此相反，并且细胞裂解液中的SOD活性显著提高（P＜0.05）。B组和C组不同程度地促进APRIL的分泌（P＜0.01），而抑制IL8的产生（P＜0.01）。B组也抑制IL10的产生，但C组却显著促进其分泌（P＜0.01）。与B组相比，D组APRIL显著减少（P＜0.01），但IL8和IL10都显著增加（P＜0.05，P＜0.01）。结果提示，L. rhamnosus可提高Caco2细胞的抗氧化能力，减轻炎症反应，具有保护作用和免疫佐剂的效果。

Abstract: In this study, the effects of Lactobacillus rhamnosus on antioxidative and nonspecific immunologic functions in Caco2 cells were investigated. Caco2 cells were treated by PBS(group A), E. coli K88(group B) and L. rhamnosus (group C), respectively. Caco2 cells in group D were preincubated with L. rhamnosus and then infected with E. coli K88. The results showed that TAOC concentration decreased（P＜0.01）, but SOD activity enhanced in cell culture supernatant（P＜0.01）in group B. However, the adverse situation appeared in group C and, SOD activity in cell lysate increased（P＜0.05）. APRIL secretion were induced（P＜0.01）, however, IL8 secretion were inhibited（P＜0.01）both in group B and group C in different degree. IL10 production was stopped in group B, and was promoted in group C（P＜0.01）. Compared with group B, APRIL production significantly decreased（P＜0.01）, whereas levels of IL8 （P＜0.05）and IL10（P＜0.01）increased in group D. These results suggested that Caco2 cells were sensitize to respond to L. rhamnosus by an improved response in antioxidative function. In addition, inflammatory response against pathogenic bacteria in enterocyte attenuated, moreover, immune response reinforced, which indicated the activities for immunoprotection and immunoadjuvant in L. rhamnosus.

黄怡;黄琴;李卫芬;余东游;周绪霞. 鼠李糖乳杆菌对Caco2细胞抗氧化功能和细胞因子分泌的影响[J]. 畜牧兽医学报, 2012, 43(2): 250-254.

HUANG Yi;HUANG Qin;LI Weifen;YU Dongyou;ZHOU Xuxia . Effects of Lactobacillus rhamnosus on Antioxidative Functions and Cytokines Secretion in Caco2 Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(2): 250-254.

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