Abstract: The aim of this study was to develop a sensitive, rapid and simple immunity colloid gold chromatographic analysis method for examination of footandmouth disease, which can make the differential diagnosis with other infectious diseases. The purified antiFMDV Asia 1 type coupling 12S antigen rabbit antibody were coupled with colloidal gold. The purified antiFMDV A, O type coupling 12S antigen rabbit antibody and goat antirabbit IgG were wrapped onto nitrocellulose membrane as the test line (T line) and the control line (C line), respectively. The FMDV general diagnosis kit was then performed. The seriate results indicated that sensitivity of the test kit reached 0.98×104LD50. No cross reaction was found with SVD, VS, VES antigen by cross tests. In the clinic test, a total 90 field samples were detected with the test list. The result of corresponding rate for positive and negative samples were 96.70% and 100%. These experiments demonstrated that the established gold immunochromatographic strip test kit was sensitive, specific for detecting FMDV and was potentially useful for first time and differential diagnosis.