马身猪和大白猪不同组织DECR1基因的表达

畜牧兽医学报 ›› 2011, Vol. 42 ›› Issue (4): 475-480.doi:

马身猪和大白猪不同组织DECR1基因的表达

李步高¹，郭晓红¹，曹果清¹，王效京²，高鹏飞¹，刘宏³，周忠孝¹*

1.山西农业大学动物科技学院，太谷 030801； 2.山西省畜禽繁育工作站，太原 030000；3.大同市种猪场，大同 037000

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-04-20 发布日期:2011-04-20
通讯作者: 周忠孝

Expression Analysis of DECR1 Gene in Tissues of Mashen and Large White Pig

LI Bu-gao¹, GUO Xiao-hong¹, CAO Guo-qing¹, WANG Xiao-jing², GAO Peng-fei¹, LIU Hong³,ZHOU Zhong-xiao¹*

1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University,Taigu 030801, China; 2. Shanxi Livestock and Poultry Breeding Station, Taiyuan 030000, China; 3. Datong Pig Breeding Farm,Datong 037000, China

Received:1900-01-01 Revised:1900-01-01 Online:2011-04-20 Published:2011-04-20

摘要/Abstract

摘要： 旨在研究DECR1基因在猪不同组织和品种中mRNA和蛋白水平的表达规律，探讨该基因与脂肪代谢的关系。以山西马身猪与大白猪为试验材料，提取肝脏、心脏、肾脏、脾脏、肺脏、胃、小肠、皮下脂肪和背最长肌组织的总RNA和总蛋白，应用实时荧光定量PCR技术检测DECR1基因在2个品种各组织中mRNA的相对表达量，采用Western blot技术对各组织中DECR1蛋白进行半定量分析。实时荧光定量PCR结果显示：DECR1基因在各组织中均有表达，组织之间的表达量存在极显著差异（P<0.01），DECR1基因在肝脏、皮下脂肪与心脏中为高丰度表达；在不同品种的皮下脂肪组织中，大白猪DECR1的mRNA表达极显著高于马身猪（P<0.01）。Western blot检测结果显示：DECR1在各组织中均有表达，不同组织的表达量存在极显著差异（P<0.01），在皮下脂肪、肝脏与小肠中高表达；不同品种的皮下脂肪组织中，大白猪DECR1蛋白的表达显著高于马身猪（P<0.05）。猪DECR1基因在不同组织的表达差异可能与脂肪代谢和脂肪沉积有关。

Abstract: The purpose of this study was to analyze DECR1 mRNA and protein expression profiles of Mashen and Large White pigs in different tissues, and evaluate the potential relationships between its relative expression and lipid metabolism. DECR1 expression profiles in nine tissues (liver, kidney, spleen, lung, small intestine, subcutaneous adipose, stomach, longissimus dorsi, heart) of Mashen and Large White pigs were examined by quantitative realtime PCR and Western blot, respectively. The results indicated that, by real-time PCR, DECR1 had the tissue-and speciesspecific in mRNA expression, but highly in liver, subcutaneous adipose and heart. The DECR1 mRNA expression in subcutaneous adipose of Large White pig was significantly higher than that of Mashen pig (P<0.01). The results showed that, by Western blot, the distribution of DECR1 in tissues were significantly different, but highly in subcutaneous adipose, liver and small intestine. Moreover, the expression of DECR1 in subcutaneous adipose of Large White pig was significantly higher than that of Mashen pig (P<0.05). It was inferred that DECR1 gene may be related to regulatory mechanism of lipid metabolism.

李步高;郭晓红;曹果清;王效京;高鹏飞;刘宏;周忠孝. 马身猪和大白猪不同组织DECR1基因的表达[J]. 畜牧兽医学报, 2011, 42(4): 475-480.

LI Bu-gao;GUO Xiao-hong;CAO Guo-qing;WANG Xiao-jing;GAO Peng-fei;LIU Hong;ZHOU Zhong-xiao . Expression Analysis of DECR1 Gene in Tissues of Mashen and Large White Pig[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2011, 42(4): 475-480.

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