鲁西牛群体遗传多样性与生长发育性状的微卫星标记研究

畜牧兽医学报 ›› 2011, Vol. 42 ›› Issue (4): 481-488.doi:

鲁西牛群体遗传多样性与生长发育性状的微卫星标记研究

毕伟伟¹，陈维云¹，王慧¹*，王中华¹，张沅²*

1.山东农业大学动物科技学院，泰安 271018； 2. 中国农业大学动物科技学院，北京 100193

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-04-20 发布日期:2011-04-20
通讯作者: 王慧；张沅

Studies on Population Genetic Diversity and Microsatellite Markers for Growth Traits in Luxi Cattle

BI Wei-wei¹，CHEN Wei-yun¹，WANG Hui¹*，WANG Zhong-hua¹，ZHANG Yuan²*

1. College of Animal Science ＆ Technology, Shandong Agricultural University, Taian 271018, China； 2. College of Animal Science ＆ Technology, ChinaAgricultural University, Beijing 100193, China

Received:1900-01-01 Revised:1900-01-01 Online:2011-04-20 Published:2011-04-20

摘要/Abstract

摘要： 以120头鲁西牛为研究对象，选用分布于牛60条染色体的30对微卫星引物中多样性丰富的8对微卫星位点，探讨与鲁西牛生长发育性状相关联的分子遗传标记。本研究采用微卫星标记技术和最小二乘线性模型，检测了鲁西牛群体的遗传多样性和分析了与鲁西牛生长发育性状相关的标记效应。结果，共检测到34个等位基因，每个微卫星座位的等位基因数为3～6个，平均等位基因数为4.25，该群体的基因平均杂合度（H）为0.561 3，多态信息含量（PIC）为0.498 3。结果表明，7个微卫星座位的不同基因型之间的差异达到显著(P<0.05）或极显著水平(P<0.01）。影响相应性状的最有利基因型分别是ETH185座位的AE与体高，BM711座位的CC和BM1824座位的BB与体长，TGLA53座位的AD和BM711座位的CC与管围，BM1824座位的BB和DVGA55座位的AB与腹围，TGLA53座位的AC、ETH185座位的BD、BM711座位的AB、DVGA46座位的AC、DVGA44座位的BB和DVGA55座位的BB与体质量。而对生长不利的基因型有：DVGA46的AD型，DVGA44的AD型，DVGA55的AA型和ETH185的BC型。研究结果为鲁西牛的标记辅助选择和早期基因型选种提供了可借鉴的分子标记。

Abstract: 8 pairs of polymorphic microsatellites out of 30 microsatellite loci in Luxi cattle chromosome were selected from GenBank. Genetic diversity of Luxi cattle and the relating effects between genotypes and growth traits were analyzed in 120 individuals by using least squares linear model and DNA analysis technique. A total of 34 alleles were detected. Each microsatellite loci in the number of alleles was ranging from 3.6, and the average number of alleles was 4.25. The average heterozygosity (H) was 0.561 3; the polymorphism information content (PIC) was 0.498 3. The results indicated that eight microsatellite loci had significant effect on some growth traits of Luxi cattle (P<0.05 or P<0.01). The most favorable genotypes for interrelated traits were AE at ETH185 for body height, CC at BM711 and BB at BM1824 for body length, AD at TGLA53 and CC at BM711 for cannon girth, BB at BM1824 and AB at DVGA55 for abdominal girth, AC at TGLA53, BD at ETH185, AB at BM711, AC at DVGA46 and BB at DVGA44 and DVGA55 for body weight, respectively While the disadvantageous genotypes were AD at microsatellite loci of DVGA46 and DVGA44, BC genotype at ETH185, AA genotype at DVGA55 loci The results make the genotype useful for markers associated with the growth traits for marker assisted selection.

毕伟伟;陈维云;王慧;王中华;张沅. 鲁西牛群体遗传多样性与生长发育性状的微卫星标记研究[J]. 畜牧兽医学报, 2011, 42(4): 481-488.

BI Wei-wei;CHEN Wei-yun;WANG Hui;WANG Zhong-hua;ZHANG Yuan. Studies on Population Genetic Diversity and Microsatellite Markers for Growth Traits in Luxi Cattle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2011, 42(4): 481-488.

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