猪细小病毒感染PK-15细胞抗病毒相关因子转录变化的分析

畜牧兽医学报 ›› 2011, Vol. 42 ›› Issue (1): 48-55.doi:

猪细小病毒感染PK-15细胞抗病毒相关因子转录变化的分析

李厚伟¹，魏战勇²，尹海燕³，陈红英², 李金磊¹，韩志涛¹，崔保安¹*

1.河南省动物性食品安全重点实验室，郑州 450002；2.河南农业大学牧医工程学院，郑州 450002；3.河南省农业科学院农业质量标准与检测技术研究中心，郑州 450002

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-01-25 发布日期:2011-01-25
通讯作者: 崔保安

The Analysis of Antiviral Cytokines Transcriptional Profiles of PK-15 Cell Cultures Following Infection with Porcine Parvovirus

LI Hou-wei¹ , WEI Zhan-yong², YIN Hai-yan³, CHEN Hong-ying², LI Jin-lei¹,
HAN Zhi-tao¹, CUI Bao-an¹*

1.Key Laboratory for Animal-derived Food Safety of Henan Province, Zhengzhou 450002, China;2.College of Animal Science and Veterinary Medicine, Henan Argricultural University, Zhengzhou 450002, China; 3.Agricultural Quality Standards and Testing Technology Research Center, Henan Academy of Agricultural Sciences， Zhengzhou 450002, China

Received:1900-01-01 Revised:1900-01-01 Online:2011-01-25 Published:2011-01-25

摘要/Abstract

摘要： 为了解猪细小病毒（PPV）感染后引起干扰素及其相关细胞因子的反应，探讨宿主—病毒之间的作用关系，作者运用荧光定量PCR技术，测定和分析PPV感染PK-15细胞引起的病毒DNA量的变化和细胞因子IFN-β、IFN-γ、IFNAR-1、IFNAR-2、MHC-Ⅰ、MHC-Ⅱ、MX1、iNOS、2-5AS、RNase L和IRF-3的转录水平。结果显示，PPV感染PK-15细胞12 h病毒开始大量迅速增殖，48 h达到最高峰；PPV感染后可引起PK-15细胞中IFN-β、IFN-γ、IFNAR-1、IFNAR-2、MHC-Ⅰ、MHC-Ⅱ、Mx1、iNOS、2-5AS、RNase L和IRF-3的转录量显著增加，其中Mx1基因在24 h转录量达到8 423倍。猪细小病毒感染可引起PK15细胞抗病毒相关因子转录增加。

Abstract: In order to survey the host inflammatory responses, particularly the antiviral cytokines responses of Porcine Parvovirus (PPV) infection, and to investigate the host-PPV interaction，Porcine Kidney-15 cells (PK-15) were infected by PPV. Then the viral DNA was measured and analyzed using real-time PCR. The transcript level of cytokines (IFN-β, IFN-γ, IFNAR-1, IFNAR-2, MHC-Ⅰ, MHC-Ⅱ, MX1, NOS, 2-5AS, RNase L and IRF-3) were detected by real-time PCR too. The results showed that PPV proliferated rapidly in PK-15 cell 12 hours after infection, and reached peak 48 hours after infection; the transcription levels of IFN-β, IFN-γ, IFNAR-1, IFNAR-2, MHC-Ⅰ, MHC-Ⅱ, MX1, iNOS, 2-5AS, RNase L and IRF-3 were increased obviously, and the transcription level of Mx1 gene was increased 8 423 times at 24 hours after infection. The results revealed that the level of antiviral cytokines increased in PPV infected PK-15 cell.

李厚伟;魏战勇;尹海燕;陈红英;李金磊;韩志涛;崔保安. 猪细小病毒感染PK-15细胞抗病毒相关因子转录变化的分析[J]. 畜牧兽医学报, 2011, 42(1): 48-55.

LI Hou-wei;WEI Zhan-yong;YIN Hai-yan;CHEN Hong-ying;LI Jin-lei;HAN Zhi-tao;CUI Bao-an . The Analysis of Antiviral Cytokines Transcriptional Profiles of PK-15 Cell Cultures Following Infection with Porcine Parvovirus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2011, 42(1): 48-55.

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