注射pGRF基因质粒对猪血清生长轴激素变化规律的影响

畜牧兽医学报 ›› 2011, Vol. 42 ›› Issue (1): 39-43.doi:

注射pGRF基因质粒对猪血清生长轴激素变化规律的影响

张金霞^1,2，张宏福¹*，周占琴²，刘雨田^1，2，刘胜军¹，卫星辉³，丁国庆³

1中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室，北京 100193；2西北农林科技大学动物科技学院，杨凌 712100；3香港格兰柏生物科技有限公司，香港 999077

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-01-25 发布日期:2011-01-25
通讯作者: 张宏福

The Effects of Injecting pGRF Gene Plasmid on Growth Axis Hormones in the Growing Pigs

ZHANG Jin-xia^{1, 2}, ZHANG Hong-fu¹*,ZHOU Zhan-qin²，LIU Yu-tian^1,2, LIU Sheng-jun¹,WEI Xing-hui³, DING Guo-qing³

1. State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China； 2.College of Animal Science and Technolgy, Northwest Agriculture and Forestry University, Yangling 712100, China; 3.Greenpak Biotech of Hong Kong, Hong Kong 999077, China

Received:1900-01-01 Revised:1900-01-01 Online:2011-01-25 Published:2011-01-25

摘要/Abstract

摘要： 本研究旨在探讨注射pGRF基因质粒在猪体内的表达效应及作用规律。试验选择8头体质量相近（（15±0.43）kg）的健康长白×大白二元杂交阉公猪，随机分成2组。试验组颈部肌肉注射含45 mg pGRF基因质粒的注射液2 mL，对照组注射生理盐水2 mL。于处理后第1、4、7、11、16、21、26、31天空腹采血样制备血清，测定血清中生长激素（GH）、类胰岛素生长因子（IGF-Ⅰ）、生长激素释放因子（GRF）、生长抑素（SS）的含量。结果表明：试验组在注射pGRF基因质粒后第7天，血清GH含量达峰值，比对照组高了113.47%，差异极显著（P＜0.01)；然后逐渐下降。到第11天时，比对照组高47.09%，差异显著（P＜0.05)。到第31天时，与对照组基本一致。试验组血清IGF-Ⅰ和GRF含量虽然提高幅度不大，但其变化与GH含量存在着显著相关性，均在注射pGRF基因质粒后第7天达到峰值，然后降至对照组水平，其相关系数分别为0.589（P＜0.05)和0.678（P＜0.05)。与对照组相比，试验组血清SS含量在11 d 前一直呈下降趋势，第11天时降至最低点，但差异不显著。到第16天时2组均有所上升，21 d 后缓慢下降，第31天时与对照组趋于一致。血清SS含量与GH、GRF的含量分别存在显著和极显著负相关，相关系数分别为－0.689（P＜0.05)和－0.777（P＜0.01)。由此可见，pGRF基因质粒在体内快速表达并作用于机体，主要通过提高GRF的含量而促使GH的分泌，同时抑制SS的分泌，一定程度阻止其对GRF和GH的抑制作用，从而达到有效的促生长作用。同时本试验发现，虽然采用了颈静脉导管方法，猪血液中生长轴激素测定值变异仍较大，加强护理和增加测试猪的数量是取得更为客观结果的必要条件。

Abstract: This study was conducted to investigate the effect of pig growth hormonereleasing factor gene plasmid （pGRF) on the change of growth axis-hormones in growing pigs by injecting. Eight crossbred castrated boar pigs (Landrace ×Large white) with similar weight((15±0.43)kg) were allocated into two treatments, the experimental and control groups. The experimental pigs was injected with 2 mL (4.5 mg) pGRF at neck, and the control ones were done in the same way with 2 mL normal saline. Serum samples were taken from all pigs at days 1, 4, 7, 11, 16, 21, 26 and 31 after injecting, and the levels of serum GH，IGF-Ⅰ，GRF and SS were determined. The results showed that the serum GH level of pigs in the experimental group increased significantly by 113.47% （P<0.01) compared with the control pigs at day 7 after injecting. Then the values declined gradually, it increased by 47.09% （P<0.05) at day 11 after injecting, and there was no significant difference at day 31. The serum IGF-Ⅰand GRF levels of the experimental pigs had no obvious increase, but they correlated significantly with serum GH level. The correlation coefficient were respectively 0.589（P<0.05) and 0.678 （P <0.05). Their peaks appeared also at day 7 and then declined gradually Compared to the control pigs, the serum SS concentrations in experimental pigs shown a downward trend before day 11 (they reached the lowest point at day 11), but they ascended at day 16 and declined slowly after that, they declined to the same level as that of control pigs at day 31. There were significant negative correlation among the serum SS and GH and GRF levels，the correlation coefficient were respectively －0.777（P<0.01) and －0.689（P<0.05). It can be seen, pGRF gene plasmid could express in vivo and promote the secretion of GH by improving the serum content of GRF. The secretion of SS was inhibited by the injection, which meant the inhibition of GRF and GH by SS was decreased to a certain degree. It suggested that pGRF gene plasmid could play a growth-promoting role in the pigs. In the same time, we found that although the blood samples were taken from pigs by venous fistula, the variances of the determined data on hormones were still big, so increasing the number of experimental pigs was necessary for the better objective results

张金霞;张宏福;周占琴;刘雨田;刘胜军;卫星辉;丁国庆. 注射pGRF基因质粒对猪血清生长轴激素变化规律的影响[J]. 畜牧兽医学报, 2011, 42(1): 39-43.

ZHANG Jin-xia;;ZHANG Hong-fu;ZHOU Zhan-qin;LIU Yu-tian;LIU Sheng-jun;WEI Xing-hui;DING Guo-qing . The Effects of Injecting pGRF Gene Plasmid on Growth Axis Hormones in the Growing Pigs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2011, 42(1): 39-43.

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