隐性乳房炎奶牛IL-8受体与乳铁蛋白基因的PCR-SSCP分析

畜牧兽医学报 ›› 2011, Vol. 42 ›› Issue (1): 136-140.doi:

隐性乳房炎奶牛IL-8受体与乳铁蛋白基因的PCR-SSCP分析

何高明，张素华，赵宗胜*，李大全

石河子大学动物科技学院，石河子 832003

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-01-25 发布日期:2011-01-25
通讯作者: 赵宗胜

Analysis on the IL-8 Receptor and Lactoferrin Genes in Subclinical Mastiticsdairy Cows by PCR-SSCP

HE Gao-ming, ZHANG Su-hua, ZHAO Zong-sheng*, LI Da-quan

College of Animal Science and Technology, Shihezi University, Shihezi 832003, China

Received:1900-01-01 Revised:1900-01-01 Online:2011-01-25 Published:2011-01-25

摘要/Abstract

摘要： 本研究旨在探讨IL-8受体基因、乳铁蛋白基因多态性与奶牛乳房炎的相互关系。试验采用PCR-SSCP技术分别对80头健康奶牛和隐性乳房炎奶牛的IL-8受体基因和乳铁蛋白基因进行了多态性检测,并分析了它们与奶牛乳汁中体细胞数的关系。PCR-SSCP分析结果表明，IL-8受体CXCR1基因SSCP-5、SSCP-7在健康奶牛个体中出现程度较高，说明其个体对隐性乳房炎表现抗性；在乳铁蛋白基因5′调控区的不同基因型中，AB基因型个体对奶牛隐性乳房炎表现抗性。

Abstract: The aim of this study was to detect the relationships between the genotypes of IL-8R and Lactoferrin genes and subclinical mastitics. The genotypes of IL8R and Lactoferrin genes of 80 healthy and 80 subclinical mastitics-dairy cows were detected by using PCRSSCP technique, and the relationships with the number of somatic cells in the milk were analysed. The results showed that the frequencies of genotype SSCP-5 and SSCP-7 of IL-8R CXCR1 were high in the healthy dairy cows and indicated that individuals with genotype AB performed resistance to subclinical-mastitics.

何高明;张素华;赵宗胜;李大全. 隐性乳房炎奶牛IL-8受体与乳铁蛋白基因的PCR-SSCP分析[J]. 畜牧兽医学报, 2011, 42(1): 136-140.

HE Gao-ming;ZHANG Su-hua;ZHAO Zong-sheng;LI Da-quan . Analysis on the IL-8 Receptor and Lactoferrin Genes in Subclinical Mastiticsdairy Cows by PCR-SSCP[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2011, 42(1): 136-140.

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