Abstract: The aim of this study was to analyze the gene expression during the goat antral follicle development. The different expressed genes in granulosa cells between non-latching large follicles (LF, 6 mm) and small follicles (SF, 4 mm) during follicular phase of Huanghuai goat ovary were studied using suppressive subtractive hybridization (SSH), and reduction cDNA library were also established. Furthermore, 96 clones randomly picked from the forward library were screened for differential expression by dot blot analysis. The results showed that eight clones had highly similar with the known genes, and twelve clones were new expressed sequence tags(ESTs). The results indicated that these genes may influence the maturation and ovulation of goat follicle.