Abstract: This study was conducted to investigate the effect of co-culture system with Vero cells on the development of porcine embryos. Ovaries were recovered from slaughterhouse, and oocytes were extracted. Oocytes were activated with electrical stimulation combined with chemical stimulation, then parthenogenetic embryos were obtained. Cloned embryos were constructed with porcine cumulus cells and oocytes. Cloned embryos and parthenogenetic embryos were co-cultured with Vero cells in NCSU-23(culture medium). The results showed that groups co-cultrued with Vero cells achieved significantly higher blastocyst formation rate compared with control(P<0.05), while there was no significant difference for the cell number of blastocysts(P>0.05). The result indicated that co-culture system with Vero cells improved the development of porcine embryos in vitro.