Abstract: To explore the method of in vitro maturation of Liaoning Cashmere goats oocytes, this experiment was carried out to study effects of cumulus cells on in vitro maturation and parthenogenetic development of oocytes. The oocytes were recovered directly from the oocytes of follicles, ＞2 mm in diameter. Experiment 1, DOs(denuded oocytes) were obtained from COCs freed of whole CCs, and then the oocytes were cultured by the following four ways: (1)COCs were cultured alone;(2) DOs were co-cultured with COCs (DOs(COCs));(3) DOs were co-cultured with CCs (DOs(CCs)); (4) DOs were cultured alone.Experiment 2, the effect of different layers of cumulus cells on the maturative ability and parthenogenetic development of oocytes were evaluated. These oocytes were divided into COCs3,COCs_1-3 and NOs according to the layers of cumulus cells.We found that there was significant difference in maturation and cleavage rates between ooctyes invested by different layers of cumulus cells, and the oocytes enclosed with more layers of cumulus cells, both maturation and cleavage rates were higher than oocytes enclosed with fewer layers of cumulus cells. The DOs(COCs) was better than DOs(CCs), and the NOs was a kind of degenerate ooctyes.Our findings would be helpful to the choice of recipient oocytes for embryonic technologies which will need high quality oocytes.