阿维菌素对体外培养王鸽脑神经细胞凋亡和一氧化氮水平的影响

畜牧兽医学报 ›› 2010, Vol. 41 ›› Issue (3): 347-352.doi:

阿维菌素对体外培养王鸽脑神经细胞凋亡和一氧化氮水平的影响

李术¹*，崔雅莉¹，王敏^1，2，徐世文¹，王俊杰¹

1. 东北农业大学动物医学学院，哈尔滨 150030；2. 黑龙江畜牧兽医职业学院，双城 150100

收稿日期:2009-11-04 修回日期:1900-01-01 出版日期:2010-03-24 发布日期:2010-03-24
通讯作者: 李术

Effects of Apoptosis and NO Level of AVM on King Pigeon Brain Cells in Vitro Culture

LI Shu ¹*, CUI Yali ¹, WANG Min^1,2, XU Shiwen¹, WANG Junjie¹

1.College of Veterinary Medicine, Northeast Agricultural University,Harbin 150030, China;2.Heilongjiang Vocational College of Animal Husbandry andVeterinary Science, Shuangcheng 150100, China

Received:2009-11-04 Revised:1900-01-01 Online:2010-03-24 Published:2010-03-24
Contact: LI Shu

摘要/Abstract

摘要： 本试验以阿维菌素染毒原代培养的王鸽脑神经细胞为模型，观察阿维菌素对神经细胞凋亡的毒性作用和一氧化氮产生量的影响。采用原位末端标记法观察细胞凋亡，四唑盐比色法检测神经细胞相对存活率，流式细胞术检测凋亡细胞百分比，分光光度法测定细胞一氧化氮的产生量。结果显示，阿维菌素可诱导神经细胞凋亡和细胞坏死，并具有随着阿维菌素剂量递增而增加的趋势，同时阿维菌素也能引起一氧化氮产生量降低，但是未发现存在剂量效应关系。

Abstract: The effects of apoptosis and NO level were explored with model of AVM on king pigeon brain neurocytes in vitro culture. Apoptosis was observed by TUNEL, the relative survival rate of neurocytes was detected by MTT, the percentage of apoptotic cells was detected by Flow cytometry, and the production of NO in cells was acquired by absorption spectrometry. The results showed that AVM can induce apoptosis and cytonecrosis of neurocyte, and has increased with the dose of AVM. AVM can reduced the production of NO, too, but no doseresponse relationship.

李术;崔雅莉;王敏;徐世文;王俊杰. 阿维菌素对体外培养王鸽脑神经细胞凋亡和一氧化氮水平的影响[J]. 畜牧兽医学报, 2010, 41(3): 347-352.

LI Shu;CUI Yali;WANG Min;XU Shiwen;WANG Junjie . Effects of Apoptosis and NO Level of AVM on King Pigeon Brain Cells in Vitro Culture[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2010, 41(3): 347-352.

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