Ghrelin免疫反应阳性细胞在鸡外周器官中的定位分布与发育性变化

畜牧兽医学报 ›› 2010, Vol. 41 ›› Issue (3): 341-346.doi:

Ghrelin免疫反应阳性细胞在鸡外周器官中的定位分布与发育性变化

魏凤梅¹，李玉谷¹*，叶远兰^1,2，张媛¹，马勇江¹，江青艳¹

1.华南农业大学兽医学院，广州 510642；2.仲恺农业工程学院，广州 510225

收稿日期:2009-09-03 修回日期:1900-01-01 出版日期:2010-03-24 发布日期:2010-03-24
通讯作者: 李玉谷

Localization and Development of Ghrelinimmunopositive Cells in Periphery Organs of Broiler Chickens

WEI Fengmei¹, LI Yugu ¹*, YE Yuanlan^1,2, ZHANG Yuan¹, MA Yongjiang ¹, JIANG Qingyan¹

1.College of Veterinary Medicine, South China Agriculture University, Guangzhou 510642, China；2. Zhong Kai University of Agriculture and Engineering, Guangzhou 510225, China

Received:2009-09-03 Revised:1900-01-01 Online:2010-03-24 Published:2010-03-24
Contact: LI Yugu

摘要/Abstract

摘要： 本试验应用免疫组织化学方法和显微图像分析技术，研究了2、16、30、44、58日龄岭南黄鸡外周器官中Ghrelin免疫反应阳性细胞的定位分布与发育性变化。结果表明，在鸡腺胃、肠、心、肝、脾、肺、肾、胰、脑垂体、肾上腺、胸腺、法氏囊中均可观察到Ghrelin免疫阳性反应。其阳性细胞类型包括：腺胃腺小管的内分泌细胞，肠道黏膜上皮和肠腺内的内分泌细胞，肠道黏膜下层和肌层间的神经丛，心内膜下层的蒲肯野纤维，肝血窦中的枯否氏细胞，脾的巨噬细胞和网状细胞，肺的巨噬细胞，肾脏肾小囊壁层的扁平上皮细胞和脏层的足细胞、球内系膜细胞和球旁复合体，胰腺的胰岛细胞，腺垂体的部分嗜酸性细胞和嗜碱性细胞，肾上腺的嗜铬细胞，胸腺上皮细胞、胸腺小体和巨噬细胞，法氏囊黏膜上皮和小结相关上皮内的内分泌细胞、囊小结的网状细胞、巨噬细胞、皮质髓质交界处的上皮细胞等。2~30日龄鸡，随着日龄的增长，各器官中的Ghrelin表达量增加，44、58日龄则有所下降。

Abstract: In order to investigate the distribution of ghrelinimmunopositive cells in chickens, some samples of broiler chickens aged 2, 16, 30, 44 and 58 d were collected and studied by immunohistochemistry and microscopic image analysis. The ghrelinimmunopositive reaction was found in the glandular stomach, intestine, heart, liver, spleen, lung, kidney, pancreas, pituitary, adrenal gland, thymus, and bursa of Fabricius. The types of ghrelinimmunopositive cells (ghrelinic) were as follows: some endocrine cells and nerve plexes in the glandular stomach and intestine; Purkinje fibers in heart; Kupffer′s cells in liver; macrophages and reticular cells in spleen; pulmonary macrophages; podocytes, Intraglomerular mesangial cells, Juxtaglomerular cells in kidney; pancreatic islets cells in pancreas; some acidophilic cells and basophilic cells in pituitary; chromaffin cells in adrenal gland; thymic epithelial cells, thymic corpuscles and macrophages in thymus; some endocrine cells, reticular cells, macrophages and epithelial cells in bursa of Fabricius. With the chicken growth, the expressive contents of ghrelin were increased from aged 2 to 30 d, but slightly decreased at aged 44 and 58 d in various organs of chickens.

魏凤梅;李玉谷;叶远兰;张媛;马勇江;江青艳. Ghrelin免疫反应阳性细胞在鸡外周器官中的定位分布与发育性变化[J]. 畜牧兽医学报, 2010, 41(3): 341-346.

WEI Fengmei;LI Yugu;YE Yuanlan;ZHANG Yuan;MA Yongjiang;JIANG Qingyan . Localization and Development of Ghrelinimmunopositive Cells in Periphery Organs of Broiler Chickens[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2010, 41(3): 341-346.

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