Abstract: To assess the role that ORF73 or ORF214 gene of fowlpox virus(FPV) played in the regulation of immune response against FPV in commercial chickens with maternal antibodies, two recombinant fowlpox viruses (rFPV) that ORF073 or ORF214 genes inserted and coexpressed the influenza haemagglutinin (rFPVLP△73LRH5A, rFPVLP△214LRH5A) and the parental recombinant virus expressing the influenza haemagglutinin (rFPVLP12LSH5A) were constructed. These viruses were assessed for their immunological efficacy on SPF chickens and commercial chickens. The results showed that the high titer of HI antibodies was induced by three rFPV, and the protective efficacies were universal 100% in SPF chickens. However, the low HI titer was induced by rFPVLP△73LRH5A or rFPVLP△214LRH5A in commercial chickens with HI maternal antibodies up to 2.45, the protective efficacies were 16.7% and 23.3%, respectively. At the same time, rFPV with deleted ORF073 or ORF214 genes in vivo were easier to eliminate than rFPVLP12LSH5A. On the other hand, in the inoculated commercial chickens without HI maternal antibodies with rFPVLP△73LRH5A or rFPVLP△214LRH5A, the HI antibody titers increased and approached to 3.50 and 3.17, respectively after 28 days. Consequently, maternal antibodies depressed immune efficacy by rFPV with deleted ORF073 or ORF214 genes (rFPVLP△73LRH5A, rFPVLP△214LRH5A) induced.