Abstract:

In order to investigate the effect of the Chinese Herbal Medicine formula Jadescreen (Yu Ping Feng San) on response to infectious bursal disease(IBD) vaccine in chickens, the birds vaccinated with IBD vaccine were feed with Jadescreen Powder at high (A) (1%), middle (B) (0.75%) and low (C) (0.5%) dosages. And some other birds were allocated to two control group, one was omitted for herbal treatment and the other was omitted for both vaccination and herbal treatment. Peripheral blood samples were taken on 21st, 28th, 35th, 42nd and 49th days from ten chickens in each group for detecting the level of antibody to IBDV. T cell subsets of CD4+ and CD8+ were determined by flow cytometry at the 21st and 35th days. Activity of phagocytosis of macrophages was determined with the experiment of carbon particles clearance at the 21st, 35th and 49th days. The results suggested that Jadescreen Powder can increase the level of antibody to IBDV. A manifests higher level of the antibody than other groups, and the difference was significant after the 28th day (P<0.05). B and C have the higher level than the controls after the 35th day (P<0.01). Jadescreen Powder can increase density of CD4+ T cell and the ratio of CD4+/ CD8+ significantly (P<0.05 or P<0.01). Jadescreen Powder can also increase the activity of phagocytosis of macrophage, and A has the better effect (P<0.05 or P<0.01). In conclusion, Jadescreen Powder can enhance both the humeral and cellular immunity in chickens challenged with IBD vaccine.