高效氯氰菊酯通过ERK1/2影响小鼠睾丸睾酮合成

畜牧兽医学报 ›› 2007, Vol. 38 ›› Issue (9): 920-925.doi:

高效氯氰菊酯通过ERK1/2影响小鼠睾丸睾酮合成

王鲜忠;吴建云;孙燕;罗英;李冠华;袁遵雪;王曦鸣;张家骅

1.西南大学动物科技学院重庆市牧草与草食家畜重点实验室，重庆 400716；2.重庆市动物卫生监督所，重庆 400000

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-09-25 发布日期:2007-09-25

β-cypermethrin Regulating the Testosterone Synthesis of Male Mouse through ERK1/2 Cascade

WANG Xian-zhong;WU Jian-yun;SUN Yan;LUO Ying;LI Guan-hua;YUAN Zun-xue; WANG Xi-ming;ZHANG Jia-hua

1. Chongqing Key Laboratory of Forage & Herbivorce,College of Animal Science, Veterinary and Aquaculture, Southwest University，Chongqing 400716, China；2.Chongqing Animal Health Supervision General Station, Chongqing 400000,China

Received:1900-01-01 Revised:1900-01-01 Online:2007-09-25 Published:2007-09-25

摘要/Abstract

摘要： 为了阐明氯氰菊酯（CP）影响睾酮合成的机制，本研究以成年SPF昆白小鼠为试验动物，通过连续7 d灌喂不同浓度的高效氯氰菊酯（β-CP），采用RT-PCR、Western blot和放射免疫分析技术分析了β-CP对睾丸重量、血浆类固醇水平、类固醇合成酶、StAR蛋白以及ERK1/2活性的影响。结果显示：随着β-CP浓度的增加，小鼠睾丸的重量无明显变化，但小鼠血浆睾酮的水平逐渐降低； P450sccmRNA、3 β-HSDmRNA和StAR mRNA的水平则无明显变化，而P450 c17 mRNA的表达明显下降； StAR蛋白的水平也呈下降趋势，但ERK1/2活性则逐渐升高。以上结果表明，β-CP通过激活ERK1/2而级联抑制P450 c 17 mRNA的转录和StAR前体蛋白的裂解，从而降低睾酮的合成。

Abstract: To study the effects of β-cypermethrin on the testosterone synthesis. Matured kunbai mouse were fed different concerntration of β-cypermethrin for 7 days RT-PCR, Western blot and radioimmunoassay(RIA) were used to analyze the ability of testosterone synthesis.The results were as followed: with the increase of β-cypermethrin concerntration,the weight of testis, the expression of P450scc mRNA ,3β-HSD mRNA and StAR mRNA had no difference between different groups.However, the expression of P450c17 mRNA and the level of StAR protein decreased significantly and the activity of ERK1/2 increased significantly. These results inferred that β- cypermethrine could inhibit the synthesis of testosterone through increasing the activity of ERK1/2 to decrease the activity of P450c17 mRNA and level of StAR Protein

王鲜忠;吴建云;孙燕;罗英;李冠华;袁遵雪;王曦鸣;张家骅. 高效氯氰菊酯通过ERK1/2影响小鼠睾丸睾酮合成[J]. 畜牧兽医学报, 2007, 38(9): 920-925.

WANG Xian-zhong;WU Jian-yun;SUN Yan;LUO Ying;LI Guan-hua;YUAN Zun-xue;WANG Xi-ming;ZHANG Jia-hua. β-cypermethrin Regulating the Testosterone Synthesis of Male Mouse through ERK1/2 Cascade[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2007, 38(9): 920-925.

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