Abstract: The aim of this experiment was to evaluate the fermentation kinetics of carbohydrate fractions (A, B1, B2 and NDS) of 7 various sections of corn stalks of “Nongda 108”, including leaf blade (LB), leaf sheath (LS), stem bark (SB), stem pith (SP), stem node (SN), whole stalk (WS) and ear husk (EH). The results showed that carbohydrate fractions were significantly different (P < 0.000 1) among various sections of corn stalks. Content of carbohydrate fractions A from high to low rank as follows: SB > SN > LB > WS > LS > EH > SP; content of carbohydrate fractions B1 as follows: EH > LS > WS > SN > LB > SP > SB; SP had the highest content of carbohydrates fraction B2, while SB had the lowest content. Gas productions of various sections of corn stalks for carbohydrates three fractions with rank from high to low values were consistent with their contents. SB and SN had the highest content of neutral detergent soluble (NDS), while SP had the lowest content, however, SN had the highest gas production, followed by EH, and SP content were the lowest. The curve subtraction technique represents a valid approach to evaluate the fermentation kinetics of carbohydrate fractions of various sections of corn stalks.