畜牧兽医学报 ›› 2007, Vol. 38 ›› Issue (10): 1093-1097.doi:

• 预防兽医 • 上一篇    下一篇

用溶血素从感染红细胞分离羊泰勒虫裂殖子

李有全;罗建勋;关贵全;马米玲;高金亮;刘志杰;党志胜;刘爱红;刘军龙;任巧云;鲁炳义;白启;殷宏

  

  1. 中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室/甘肃省动物寄生虫病重点实验室,兰州 730046
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-10-25 发布日期:2007-10-25

Purification of Merozoites of Theileria spp. from Infected Erythrocytes in Sheep Using Hemolysin

LI You-quan;LUO Jian-xun;GUAN Gui-quan;MA Mi-ling;GAO Jin-liang;LIU Zhi-jie;
DANG Zhi-sheng;LIU Ai-hong;LIU Jun-long;REN Qiao-yun;LU Bing-yi;BAI Qi;YIN Hong
  

  1. Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences,Lanzhou 730046, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-10-25 Published:2007-10-25

摘要: 用嗜水气单胞菌产生的细胞毒素—溶血素Ah-1,裂解羊泰勒虫感染的红细胞,通过Percoll密度梯度离心分离羊泰勒虫裂殖子,建立了一种自感染红细胞中分离泰勒虫裂殖子的快速方法。光学显微镜观察显示,所分离的羊泰勒虫裂殖子基本保留原有的正常形态,并从宿主细胞成分中游离。

Abstract: A simple and rapid method for purifying merozoites of Theileria spp. from infected erythrocytes in sheep or goats was developed. Infected erythrocytes were effectively lysed using hemolysin Ah-1 produced by Aeromonas hydrophila, and the lysate was subjected to centrifugation in a Percoll discontinuous density gradient at common speed. Light microscopical study showed that the merozoites purified retained their intact basic structure and were free of infected erythrocyte membrane and other host-cell components