二重PCR检测猪、鸡源致病性大肠杆菌、沙门氏菌磺胺类耐药基因（Sul1、Sul2、Sul3）的研究

畜牧兽医学报 ›› 2007, Vol. 38 ›› Issue (10): 1088-1092.doi:

二重PCR检测猪、鸡源致病性大肠杆菌、沙门氏菌磺胺类耐药基因（Sul1、Sul2、Sul3）的研究

羊云飞;王红宁;谭雪梅;张安云;杨鑫;夏青青;曾博

1四川农业大学动物科技学院，雅安 625014；2 四川大学生命科学学院四川大学动物疾病防控生物工程研究中心，四川大学“985工程”西南资源环境与灾害防治科技创新平台，成都 610064；3成都市动物防疫监督总站，成都 610014

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-10-25 发布日期:2007-10-25

Detection of SulfonamidesResistant Genes of Pathogenic Salmonella and Ecoli by Duplex PCR

YANG Yun-fei;WANG Hong-ning;TAN Xue-mei;ZHANG An-yun;YANG Xin;XIA Qing-qing;ZENG Bo

1College of Animal Science and Technology，Sichuan Agricultural University，Yaan 625014，China;2Sichuan University “985” Project, College of Life Science, Sichuan University，Chengdu 610064, China; 3The Station of Supervising Animal Epidemic Prevention of Chengdu, Chengdu 610014,China

Received:1900-01-01 Revised:1900-01-01 Online:2007-10-25 Published:2007-10-25

摘要/Abstract

摘要： 利用二重PCR对临床分离的猪、鸡源致病性大肠杆菌103株和沙门氏菌33株的3个磺胺类药主要耐药基因Sul1、Sul2、 Sul3进行了检测。结果表明，在19株耐SD的沙门氏菌中有17株检测出Sul1或Sul2基因，检出率为89.47%,与药敏试验的结果相比，符合率为89.47%；在56株耐SD的大肠杆菌中有53株检出耐药基因，与药敏试验的符合率为94.64%。二重PCR方法与常规PCR的结果符合率为98.5%，具有很高的一致性；与药敏试验也有很好的符合率，具有较高的特异性。整个试验过程只需7 h，比常规PCR节约7 h，检测速度有了较大的提高。

Abstract: The Sulfonamides-resistant genes Sul1 ，Sul2， Sul3 were detected by duplex PCR from 33 Salmonella strains and 103 E.coli strains from pig and chicken The results showed that Sul1,Sul2 genes of 17 Salmonella strains were detected from 19 SD resistant Salmonella strains demonstrated by MIC, and the detection rate was 89.47%. The coincidence rate between duplex PCR assays and MIC assays was 89.47%. Sul1 ，Sul2 genes of 53 E.coli strains were detected from 56 SD resistant E.coli strains. The rate of coincidence of duplex PCR assays and MIC assays was 94.64%.The rate of coincidence between duplex PCR assays and common PCR assays is 98.5%. It was only taken 7 hours to complete the whole duplex PCR test.

羊云飞;王红宁;谭雪梅;张安云;杨鑫;夏青青;曾博. 二重PCR检测猪、鸡源致病性大肠杆菌、沙门氏菌磺胺类耐药基因（Sul1、Sul2、Sul3）的研究[J]. 畜牧兽医学报, 2007, 38(10): 1088-1092.

YANG Yun-fei;WANG Hong-ning;TAN Xue-mei;ZHANG An-yun;YANG Xin;XIA Qing-qing;ZENG Bo. Detection of SulfonamidesResistant Genes of Pathogenic Salmonella and Ecoli by Duplex PCR[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2007, 38(10): 1088-1092.

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