用单胚mRNA差显技术克隆山羊早期胚胎发育相关基因

畜牧兽医学报 ›› 2006, Vol. 37 ›› Issue (9): 924-927.doi:

用单胚mRNA差显技术克隆山羊早期胚胎发育相关基因

李拥军; 敖红; 孙桂金

1.扬州大学动物科学与技术学院，扬州 225009；2.中国农业科学院北京畜牧兽医研究所，北京 100094；3. 山东农业大学动物科学与技术学院，泰安 271018

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-09-25 发布日期:2006-09-25

Cloning of Goat Early Embryonic-Developmental Gene Using Single Preimplantation Embryo mRNA Differential Display

LI Yong-jun; AO Hong; SUN Gui-jin

1.College of Animal Science & Technology, Yangzhou University, Yangzhou 225009, China;2.Insitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China;3. College of Animal Science & Technology, Shandong Agricultural University, Taian 271018, China

Received:1900-01-01 Revised:1900-01-01 Online:2006-09-25 Published:2006-09-25

摘要/Abstract

摘要： 用单胚构建的mRNA差异显示技术，对体外培养的山羊早期2、4、8-16细胞期胚胎的基因表达进行了研究，并选择了一条在4细胞期胚胎特异表达的条带进行分析。结果表明：该片段与牛胰岛素样生长因子结合蛋白3（IGFBP3）基因具有88％的同源性。该基因通过调控IGFs的生物学活性而影响早期胚胎的生长发育，并具有促进细胞分裂和影响内分泌的作用，是羊早期胚胎发育过程中的重要调控因素。

Abstract: Single preimplantation embryo mRNA differential display was used to study the gene expression of 2-cell、4-cell and 8-16-cell stage goat embryos cultured in vitro. A specific fragment expressed in 4-cell stage was selected for further analysis. The result suggested that this fragment displayed high homology (88%) to cattle insulinlike growth factor binding protein-3 (IGFBP3) gene. IGFBP3 was the regulator of IGFs bioactivity, it can also promote cell division and influence endocrine activation. IGFBP3 was an important factor in the process of goat preimplantation embryo development.

李拥军;敖红; 孙桂金. 用单胚mRNA差显技术克隆山羊早期胚胎发育相关基因[J]. 畜牧兽医学报, 2006, 37(9): 924-927.

LI Yong-jun;AO Hong;SUN Gui-jin. Cloning of Goat Early Embryonic-Developmental Gene Using Single Preimplantation Embryo mRNA Differential Display[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2006, 37(9): 924-927.

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