Abstract: PRKAG3 gene, which encodes AMP-activated protein kinase(AMPK)γ3 subunit, was considered as a major gene of affecting meat quality traits such as muscle pH, color and water-holding capacity, and its nonconservative substitution(R200Q) associated with excess glycogen content in pig skeletal muscle is the reason of causing RN^- effect. In present study, semi-quantitative RT-PCR was applied to determine the PRKAG3 gene expression difference in heart, liver, kidney and skeletal muscle of Ya-Nan and DLY pigs, and the data were normalized using the β-actin gene as internal control. Additionally, carcass traits, including undressing percentage, backfat thickness , eye lean area and intramuscular fat were measured. The results suggested that PRKAG3 was mainly expressed in skeletal muscle and less in heart，but not in liver and kidney. In skeletal muscle, PRKAG3 gene expression of Ya-Nan pigs was higher than that of DLY pigs（P=0.078）.Correlation analyses showed that the relative expression of PRKAG3 gene had no relation to undressing percentage, backfat thickness and eye lean area, but had positive relation to intramuscular fat（r=0.832）