家鹅Myostatin 基因3′-UTR序列分析

畜牧兽医学报 ›› 2006, Vol. 37 ›› Issue (6): 550-554.doi:

家鹅Myostatin 基因3′-UTR序列分析

杨凤萍;王金玉;陈义权;李其松;耿士忠;张柳;沈华

1.扬州大学生物科学与技术学院，扬州 225009;2.扬州大学动物科学与技术学院，扬州 225009

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-06-25 发布日期:2006-06-25

Sequence Analysis of 3′-UTR of Myostatin Gene in Domestic Goose

YANG Feng-ping;WANG Jin-yu; CHEN Yi-quan;LI Qi-song;GEN Shi-zhong;ZHANG Liu;SHEN Hua

1.College of Bioscience and Technology, Yangzhou University, Yangzhou 225009，China;2.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009，China

Received:1900-01-01 Revised:1900-01-01 Online:2006-06-25 Published:2006-06-25

摘要/Abstract

摘要： 利用家鹅Myostatin基因第3外显子设计上游引物、家鸡Myostatin 3′-UTR设计下游引物，PCR扩增并克隆出家鹅该基因 3′-UTR长1 064 bp DNA序列。该序列具有一个polyA 加尾信号序列(TAATAAA)，并富含连续的TTTT高保守区。分析家鹅与其他12个物种该序列的同源性发现，其与家鸡的同源性最高，达87.1%，与鱼类的最低（<11%)。以13个物种Myostatin 3′-UTR构建的分子进化树表明，3′UTR能够为动物进化提供一定信息，但适用于较低分类阶元。

Abstract: A 3′-untranslated region (UTR) DNA sequence (1 064 bp) of Myostatin gene in goose was obtained by PCR, using forward primer devised by Myostatin gene of the goose and reverse primer devised by Myostatin gene of the chicken. It has a polyA tail-signal sequence (TAATAAA) and highfrequency TTTT sequences (a kind of high conservative region). Compared with 12 other species’ same gene sequence, it has the highest homology with chicken (up to 87.1%) and the lowest homology with fishes (down to 11%). The phylogenetic tree constructed by 3′-UTR of Myostatin genes in these 13 species shows that 3′-UTR sequence can offer certain information for animal’s evolution.

杨凤萍;王金玉;陈义权;李其松;耿士忠;张柳;沈华. 家鹅Myostatin 基因3′-UTR序列分析[J]. 畜牧兽医学报, 2006, 37(6): 550-554.

YANG Feng-ping;WANG Jin-yu;CHEN Yi-quan;LI Qi-song;GEN Shi-zhong;ZHANG Liu;SHEN Hua. Sequence Analysis of 3′-UTR of Myostatin Gene in Domestic Goose[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2006, 37(6): 550-554.

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