狗舌草提取物诱导L1210细胞的凋亡

畜牧兽医学报 ›› 2006, Vol. 37 ›› Issue (3): 295-298.doi:

狗舌草提取物诱导L1210细胞的凋亡

陈进军;王建华

1．广东海洋大学农学院动物医学系，湛江 524088；2．西北农林科技大学动物科技学院，杨凌 712100

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-03-25 发布日期:2006-03-25

Apoptosis of L1210 Cell Line Induced by Extract from Tephroseris Kirilowii Turcz Holub in vitro

CHEN Jin-jun;WANG Jian-hua

1．Department of Veterinary Medicine, Agricultural College, Guangdong Ocean University, Zhanjiang 524088,China；2．College of Animal Sciences, Northwest Sci-Tech University of Agriculture & Forestry, Yangling 712100,China

Received:1900-01-01 Revised:1900-01-01 Online:2006-03-25 Published:2006-03-25

摘要/Abstract

摘要： 以环磷酰胺、槲皮素和单猪屎豆碱为参照，观察狗舌草60%乙醇提取物体外对L1210细胞的凋亡形态变化的影响；利用流式细胞术Annexin Ⅴ-FITC/PI双参数法，研究狗舌草60%乙醇提取物对L1210细胞早期凋亡的影响，探讨狗舌草60%乙醇提取物致L1210细胞凋亡的机理。结果发现，狗舌草60%乙醇提取物能使L1210细胞发生典型的细胞凋亡，形成凋亡小体；狗舌草60%乙醇提取物引起的Annexin Ⅴ-FITC⁺/PI细胞百分比为86.8%，能诱导L1210细胞发生早期凋亡。

Abstract: With a reference system of cyclophosphamide (CY), quercetin (QU) and monocrotaline (MO), the apoptotic cellular morphology of murine L1210 induced by the extract from Tephroseris kirilowii Turcz Holub with 60% ethanol solution (EX) was observed in vitro. The early apoptotic actions of EX to murine L1210 cells were studied by Annexin Ⅴ-FITC/PI of flow cytometry and the apoptotic mechanism of EX to murine L1210 was discussed. The results showed that EX at 1.0μg/mL made murine L1210 cells become apoptotic bodies in morphology and EX induced murine L1210 cells into early apoptosis with 86.8% Annexin Ⅴ-FITC⁺/PI^-cells.

陈进军;王建华. 狗舌草提取物诱导L1210细胞的凋亡 [J]. 畜牧兽医学报, 2006, 37(3): 295-298.

CHEN Jin-jun;WANG Jian-hua. Apoptosis of L1210 Cell Line Induced by Extract from Tephroseris Kirilowii Turcz Holub in vitro[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2006, 37(3): 295-298.

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