Abstract: The capsid protein gene, 1BCD, of swine vesicular disease virus (SVDV) was amplified by RT-PCR using high fidelity DNA polymerase and cloned into alphavirus repliconbased plasmid, namely suicidal plasmid, pSCA1 The recombinant plasmid was checked by restriction enzyme analysis and nucleic acid sequencing, and right constructed plasmid was named pSCA/1BCD. Then the recombinant plasmid was transfected into BHK-21 cells by Lipofectamine PlusTM reagent. The capsid proteins of SVDV expressed in BHK-21 cells were confirmed by RT-PCR and indirect immunofluorescence test. Immunization of guinea pig with suicidal DNA vaccine, pSCA/1BCD induced SVDV specific antibody and proliferation of PMBC.