畜牧兽医学报 ›› 2005, Vol. 36 ›› Issue (4): 362-364.doi:

• 兽医 • 上一篇    下一篇

镉致鸡脾脏淋巴细胞DNA损伤的研究

李金龙;熊永忠;徐世文;王秀荣;李 术   

  1. 1.东北农业大学动物医学院,哈尔滨 150030； 2.中国农业科学院哈尔滨兽医研究所, 农业部动物流感重点开放实验室及兽医生物技术国家重点实验室,哈尔滨 150001
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2005-04-25 发布日期:2005-04-25

Effect of Cadmium on DNA Damage in Splenic Lymphocytes of Chicken

LI Jin-long;XIONG Yong-zhong;XU Shi-wen;WANG Xiu-rong;LI Shu
  

  1. 1.Department of Veterinary Medicine, Northeast Agricultual University, Harbin 150030,China;2. Animal Influenza Laboratory of Ministry of Agriculture and National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of CAAS, Harbin 150001, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2005-04-25 Published:2005-04-25

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摘要： 应用片段化DNA定量分析法和碱性单细胞凝胶电泳法，检测了10 μmol/L镉对体外不同时间培养的鸡脾脏淋巴细胞DNA损伤的情况，结果表明：10 μmol/L的镉能够明显损伤鸡脾脏淋巴细胞DNA，并且呈现时间效应。提示镉致淋巴细胞DNA损伤是镉对禽类免疫毒性的重要机制之一。

Abstract: Quantative analysis of DNA fragmetation assay and alkaline single cell gel electrophoresis test were used to detect DNA damage of chicken splenic lymphocytes, cultured in vitro, which was induced by cadmium. The results showed that cadmium with the concentration of 10 μmol/L can induce DNA damage of chicken splenic lymphocytes obviously, which showed timedependent model. It demonstrated that DNA damage of lymphocytes induced by cadmium may be one of important mechanisms of avian immunotoxicity on cadmium.

ISSN 0366-6964
CN 11-1985/S
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