表没食子儿茶素没食子酸酯对牛冷冻精液品质及受精能力的影响

doi:10.11843/j.issn.0366-6964.2018.10.011

摘要/Abstract

摘要：

本试验旨在探究牛冷冻-解冻精液中添加不同浓度表没食子儿茶素没食子酸酯（epigallocatechin-3-gallate，EGCG）对精液品质及受精能力的影响。在牛冷冻-解冻精液中添加不同浓度（0、5、10和20 μmol·L^-1）EGCG，38.5℃孵育1 h后，检测精子动力学参数、结构完整性、精子中相关酶活力和体外受精能力。结果表明，10 μmol·L^-1 EGCG处理显著提高了精子活力（TM，%）和鞭打频率（BCF，Hz，P<0.05）；与对照组相比，5、10和20 μmol·L^-1 EGCG处理组精子质膜和顶体完整率显著升高（P<0.05）；5和10 μmol·L^-1 EGCG处理组精子线粒体膜完整率显著升高（P<0.05）；10和20 μmol·L^-1 EGCG处理能够显著提高精子中超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活力，10 μmol·L^-1EGCG处理降低乳酸脱氢酶（LDH）活力和丙二醛（MDA）含量（P<0.05）；10和20 μmol·L^-1 EGCG处理显著提高了精子受精后早期胚胎卵裂率和囊胚率（P<0.05）。综上表明，10 μmol·L^-1 EGCG处理牛冷冻-解冻精液能提高精液品质及受精能力。

Abstract:

The present study aimed to investigate the effects of epigallocatechin-3-gallate (EGCG) on bovine frozen-thawed semen quality and subsequent fertilization ability. The frozen-thawed semen were incubated with different concentrations of EGCG(0, 5, 10 and 20 μmol·L^-1) at 38.5℃ for 1 h. Kinetic parameters, structural integrity, activities of related enzymes and subsequent fertilization ability were measured. The results revealed that supplementation with 10 μmol·L^-1 EGCG significantly improved the percentage of total motile sperms(TM,%) and the beat cross frequency (BCF, Hz, P<0.05). The percentages of spermatozoa with intact plasma membrane and acrosome were significantly higher in 5, 10 and 20 μmol·L^-1 EGCG treated groups compared with the control group(P<0.05). 5 and 10 μmol·L^-1 EGCG led to higher mitochondrial membrane integrity(P<0.05). The activities of SOD and CAT in spermatozoa treated with 10 and 20 μmol·L^-1 EGCG were significantly increased, while the LDH activity and MDA level were markedly decreased in 10 μmol·L^-1 EGCG group(P<0.05). The cleavage and blastocyst development rates of in vitro-produced bovine embryos originating from 10 and 20 μmol·L^-1 EGCG-treated spermatozoa were significantly higher than for embryos from other groups(P<0.05). In summary, 10 μmol·L^-1 EGCG can enhance the quality and subsequent fertilization ability of bovine frozen-thawed semen.

中图分类号:

S823.2

黄自强, 王玲玲, 庞云渭, 郝海生, 杜卫华, 赵学明, 刘岩, 朱化彬. 表没食子儿茶素没食子酸酯对牛冷冻精液品质及受精能力的影响[J]. 畜牧兽医学报, 2018, 49(10): 2154-2162.

HUANG Zi-qiang, WANG Ling-ling, PANG Yun-wei, HAO Hai-sheng, DU Wei-hua, ZHAO Xue-ming, LIU Yan, ZHU Hua-bin. Effects of Epigallocatechin-3-gallate on Bovine Frozen Semen Quality and Subsequent Fertilization Ability[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(10): 2154-2162.

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参考文献

[1] COLÁS C,JUNQUERA C,PÉREZ-PÉ R,et al.Ultrastructural study of the ability of seminal plasma proteins to protect ram spermatozoa against cold-shock[J].Microsc Res Tech,2009,72(8):566-572.
[2] AGARWAL A,SHARMA R K,NALLELLA K P,et al.Reactive oxygen species as an independent marker of male factor infertility[J].Fertil Steril,2006,86(4):878-885.
[3] SUCCU S,BERLINGUER F,PASCIU V,et al.Melatonin protects ram spermatozoa from cryopreservation injuries in a dose-dependent manner[J].J Pineal Res,2011,50(3):310-318.
[4] PAUDEL K P,KUMAR S,MEUR S K,et al.Ascorbic acid,catalase and chlorpromazine reduce cryopreservation-induced damages to crossbred bull spermatozoa[J].Reprod Domest Anim,2010,45(2):256-262.
[5] BUCAK M N,ATAMAN M B,BASPINAR N,et al.Lycopene and resveratrol improve post-thaw bull sperm parameters:sperm motility,mitochondrial activity and DNA integrity[J].Andrologia,2015,47(5):545-552.
[6] KIM J E,SHIN M H,CHUNG J H.Epigallocatechin-3-gallate prevents heat shock-induced MMP-1 expression by inhibiting AP-1 activity in human dermal fibroblasts[J].Arch Dermatol Res,2013,305(7):595-602.
[7] PETER B,BOSZE S,HORVATH R.Biophysical characteristics of proteins and living cells exposed to the green tea polyphenol epigallocatechin-3-gallate (EGCG):review of recent advances from molecular mechanisms to nanomedicine and clinical trials[J].Eur Biophys J,2017,46(1):1-24.
[8] OLIVA J,BARDAG-GORCE F,TILLMAN B,et al.Protective effect of quercetin,EGCG,catechin and betaine against oxidative stress induced by ethanol in vitro[J].Exp Mol Pathol,2011,90(3):295-299.
[9] KIM H S,QUON M J,KIM J A.A New insights into the mechanisms of polyphenols beyond antioxidant properties;lessons from the green tea polyphenol,epigallocatechin 3-gallate[J].Redox Biol,2014,2:187-195.
[10] GUPTA J,SIDDIQUE Y H,BEG T,et al.A review on the beneficial effects of tea polyphenols on human health[J].Int J Pharmacol,2008,4(5):314-338.
[11] DING J,WANG H,WU Z B,et al.Protection of murine spermatogenesis against ionizing radiation-induced testicular injury by a green tea polyphenol[J].Biol Reprod,2015,92(1):6.
[12] ROYCHOUDHURY S,AGARWAL A,VIRK G,et al.Potential role of green tea catechins in the management of oxidative stress-associated infertility[J].Reprod Biomed Online,2017,34(5):487-498.
[13] DE AMICIS F,SANTORO M,GUIDO C,et al.Epigallocatechin gallate affects survival and metabolism of human sperm[J].Mol Nutr Food Res,2012,56(11):1655-1664.
[14] GADANI B,BUCCI D,SPINACI M,et al.Resveratrol and Epigallocatechin-3-gallate addition to thawed boar sperm improves in vitro fertilization[J].Theriogenology,2017,90:88-93.
[15] LI W,ZHU S,LI J H,et al.EGCG stimulates autophagy and reduces cytoplasmic HMGB1 levels in endotoxin-stimulated macrophages[J].Biochem Pharmacol,2011,81(9):1152-1163.
[16] PANG Y W,SUN Y Q,JIANG X L,et al.Protective effects of melatonin on bovine sperm characteristics and subsequent in vitro embryo development[J].Mol Reprod Dev,2016,83(11):993-1002.
[17] SHEN T,JIANG Z L,LIU H,et al.Effect of Salvia miltiorrhiza polysaccharides on boar spermatozoa during freezing-thawing[J].Anim Reprod Sci,2015,159:25-30.
[18] PANG Y W,SUN Y Q,SUN W J,et al.Melatonin inhibits paraquat-induced cell death in bovine preimplantation embryos[J].J Pineal Res,2016,60(2):155-166.
[19] ROSENKRANS C F Jr,FIRST N L.Effect of free amino acids and vitamins on cleavage and developmental rate of bovine zygotes in vitro[J].J Anim Sci,1994,72(2):434-437.
[20] KODAMA H,KURIBAYASHI Y,GAGNON C.Effect of sperm lipid peroxidation on fertilization[J].J Androl,1996,17(2):151-157.
[21] KITAJI H,OOKUTSU S,SATO M,et al.Preincubation with green tea polyphenol extract is beneficial for attenuating sperm injury caused by freezing-thawing in swine[J].Anim Sci J,2015,86(11):922-928.
[22] KIKUCHI K,NAGAI T,KASHIWAZAKI N,et al.Cryopreservation and ensuing in vitro fertilization ability of boar spermatozoa from epididymides stored at 4℃[J].Theriogenology,1998,50(4):615-623.
[23] ADEWOYIN M,IBRAHIM M,ROSZAMAN R,et al.Male infertility:the effect of natural antioxidants and phytocompounds on seminal oxidative stress[J].Diseases,2017,5(1):9.
[24] GADEA J,SELLÉS E,MARCO M A,et al.Decrease in glutathione content in boar sperm after cryopreservation:effect of the addition of reduced glutathione to the freezing and thawing extenders[J].Theriogenology,2004,62(3-4):690-701.
[25] 曹念,许建强,戴阅,等.EGCG对大鼠肺缺血再灌注损伤的保护作用研究[J].临床肺科杂志,2018,23(2):292-295.
CAO N,XU J Q,DAI Y,et al.Protective effect study of EGCG on lung ischemia-reperfusion injury[J].Journal of Clinical Pulmonary Medicine,2018,23(2):292-295.(in Chinese)
[26] 贺音.EGCG缓解丙烯酰胺诱导的大鼠肝及神经损伤机理研究[D].沈阳:沈阳农业大学,2017.
HE Y.Study on the mechanism of epigallocatechin-3-gallate on alleviating acrylamide-induced liver and nerve damage in rats[D].Shenyang:Shenyang Agricultural University,2017.(in Chinese)
[27] 刘颖.茯苓多糖、黄芪多糖和银杏叶黄酮对猪精子冷冻保存效果的研究[D].杨凌:西北农林科技大学,2014.
LIU Y.Effect of pachymaran,APS and ginkgo flavones on cryopreservation of boar sperm[D].Yangling:Northwest A&F University,2014.(in Chinese)
[28] 杨健.绵羊精液稀释液添加PUFA对精子冷冻保护效果研究[D].呼和浩特:内蒙古农业大学,2006.
YANG J.The research to protecting effect of frozen semen in sheep semen diluent with supplementation of PUFA[D].Huhhot:Inner Mongolia Agricultural University,2006.(in Chinese)
[29] 王锋,刘季春,何明.表没食子儿茶素没食子酸酯预处理对乳鼠心肌细胞缺氧/复氧损伤保护及相关机制的研究[J].江西医学院学报,2009,49(2):14-17.
WANG F,LIU J C,HE M.Study of preconditioning with epigallocatechin gallate protects cultured neonatal rat myocardial cells against anoxia/reoxygenation injury[J].Acta Academiae Medicinae Jiangxi,2009,49(2):14-17.(in Chinese)
[30] WEI D,ZHANG X L,WANG Y Z,et al.Lipid peroxidation levels,total oxidant status and superoxide dismutase in serum,saliva and gingival crevicular fluid in chronic periodontitis patients before and after periodontal therapy[J].Aust Dent J,2010,55(1):70-78.
[31] GUVVALA P R,RAVINDRA J P,RAJANI C V,et al.Protective role of epigallocatechin-3-gallate on arsenic induced testicular toxicity in Swiss albino mice[J].Biomed Pharmacother,2017,96:685-694.
[32] 任仲坤,张志毕,刘艳红,等.EGCG抗糖尿病心肌病小鼠心肌组织中氧化应激损伤作用机制的探讨[J].中国实验方剂学杂志,2018,24(3):148-153.
REN Z K,ZHANG Z B,LIU Y H,et al.Anti-inflammatory and anti-oxidative stress damage of catechin gallate on diabetic myocardial in C57 mice[J].Chinese Journal of Experimental Traditional Medical Formulae,2018,24(3):148-153.(in Chinese)
[33] HYON S H.A non-frozen living tissue bank for allotransplantation using green tea polyphenols[J].Yonsei Med J,2004,45(6):1025-1034.