骨碎补总黄酮对缺氧环境中犬骨髓间充质干细胞成骨分化潜能的影响

doi:10.11843/j.issn.0366-6964.2022.04.028

Abstract

Abstract: This study aims at studying the effects of the total flavonoids of Drynaria Rhizoma (TFDR) on osteogenic differentiation potential of canine bone marrow mesenchymal stem cells (BMSCs) in hypoxic. TFDR was used to intervene canine BMSCs in a low oxygen concentration (10%) environment for 4 weeks to induce osteogenic differentiation. Observation of calcium nodule formation in BMSCs stained with Alizarin Red by an inverted microscope. The alkaline phosphatase (ALP) activity level was detected by colorimetric method. The cell mitochondrial membrane potential was detected by flow cytometry. The expression of Runx2 and Osterix, the key genes for osteogenic differentiation were detected by RT-PCR and confocal laser microscope. Results were as follows: Compared with the control group, 10% oxygen concentration inhibited the formation of calcium nodules and ALP activity in canine BMSCs (P<0.01), Mitochondrial membrane potential level dropped (P<0.05), and the expression of Runx2 and Osterix decreased (P<0.05 or P<0.01). Compared with the hypoxic group, TFDR can significantly promoted the formation of calcium nodules and ALP activity of BMSCs in Hypoxic (P<0.05), the level of cell mitochondrial membrane potential increased (P<0.05), and Runx2 and Osterix expression increased (P<0.05 or P<0.01). TFDR can promote the osteogenic differentiation of canine BMSCs in hypoxic.

Key words: hypoxic environment, canine bone marrow mesenchymal stem cells, total flavonoids of Drynariae Rhizoma, osteogenic differentiation

CLC Number:

S853.74

LONG Yali, TIAN Qihui. Effects of Total Flavonoids of Drynariae Rhizoma on Osteogenic Differentiation Potential of Canine Bone Marrow Mesenchymal Stem Cells in Hypoxic Environment[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(4): 1280-1288.

References 19

[1]	ZHANG P, ZHANG H G, LIN J L, et al. Insulin impedes osteogenesis of BMSCs by inhibiting autophagy and promoting premature senescence via the TGF-β1 pathway[J]. Aging (Albany NY), 2020, 12(3):2084-2100.
[2]	CHEN G L, ZHUO Y J, TAO B, et al. Moderate SMFs attenuate bone loss in mice by promoting directional osteogenic differentiation of BMSCs[J]. Stem Cell Res Ther, 2020, 11(1):487.
[3]	HAMEISTER R, LOHMANN C H, DHEEN S T, et al. Bone biology in postnatal Wistar rats following hypoxia-reoxygenation[J]. Histol Histopathol, 2020, 35(1):111-124.
[4]	XING J Y, YING Y Q, MAO C X, et al. Hypoxia induces senescence of bone marrow mesenchymal stem cells via altered gut microbiota[J]. Nat Commun, 2018, 9(1):2020.
[5]	HOU K, LI G C, ZHAO J C, et al. Bone mesenchymal stem cell-derived exosomal microRNA-29b-3p prevents hypoxic-ischemic injury in rat brain by activating the PTEN-mediated Akt signaling pathway[J]. J Neuroinflammation, 2020, 17(1):46.
[6]	王军松, 李佳, 刘桂奇. 骨碎补总黄酮对大鼠骨髓间充质干细胞倍增反应和分化的作用机制实验[J]. 解放军医学院学报, 2021, 42(2):202-206.WANG J S, LI J, LIU G Q. Mechanism of total flavonoids of rhizoma drynariae in multiplication response and differentiation of BMSCs in rats[J]. Academic Journal of Chinese Pla Medical School, 2021, 42(2):202-206. (in Chinese)
[7]	朱庭辰, 华臻, 殷杰, 等. 补肾中药促进成骨前体细胞MC3T3-E1成骨与分化研究进展[J]. 安徽中医药大学学报, 2019, 38(6):92-96.ZHU T C, HUA Z, YIN J, et al. Kidney-tonifying Chinese medicine promotes osteogenic precursor cells MC3T3-E1 research progress of osteogenic and differentiation[J]. Journal of Anhui University of Chinese Medicine, 2019, 38(6):92-96. (in Chinese)
[8]	伍志伟, 刘丹, 薛娜, 等. 黄芪多糖对低氧环境中BMSCs成骨分化的影响[J]. 中国细胞生物学学报, 2017, 39(6):732-738.WU Z W, LIU D, XUE N, et al. Effects of astragalus polysaccharides on osteogenic differentiation of BMSCs cultured in low oxygen environment[J]. Chinese Journal of Cell Biology, 2017, 39(6):732-738. (in Chinese)
[9]	OUYANG Z X, TAN T T, ZHANG X H, et al. CircRNA hsa_circ_0074834 promotes the osteogenesis-angiogenesis coupling process in bone mesenchymal stem cells (BMSCs) by acting as a ceRNA for miR-942-5p[J]. Cell Death Dis, 2019, 10(12):932.
[10]	SHA Y Q, LV Y G, XU Z L, et al. MGF E peptide pretreatment improves the proliferation and osteogenic differentiation of BMSCs via MEK-ERK1/2 and PI3K-Akt pathway under severe hypoxia[J]. Life Sci, 2017, 189:52-62.
[11]	YU X, WAN Q L, YE X L, et al. Cellular hypoxia promotes osteogenic differentiation of mesenchymal stem cells and bone defect healing via STAT3 signaling[J]. Cell Mol Biol Lett, 2019, 24:64.
[12]	FU L, PENG S F, WU W F, et al. LncRNA HOTAIRM1 promotes osteogenesis by controlling JNK/AP-1 signalling-mediated RUNX2 expression[J]. J Cell Mol Med, 2019, 23(11):7517-7524.
[13]	ZOU L, KIDWAI F K, KOPHER R A, et al. Use of RUNX2 expression to identify osteogenic progenitor cells derived from human embryonic stem cells[J]. Stem Cell Reports, 2015, 4(2):190-198.
[14]	ZHANG C. Molecular mechanisms of osteoblast-specific transcription factor Osterix effect on bone formation[J]. J Peking Univ (Health Sci), 2012, 44(5):659-665.
[15]	XIAO W L, ZHANG D Z, FAN C H, et al. Intermittent stretching and osteogenic differentiation of bone marrow derived mesenchymal stem cells via the p38 mapk-osterix signaling pathway[J]. Cell Physiol Biochem, 2015, 36(3):1015-1025.
[16]	ZHANG H B, MENZIES K J, AUWERX J. The role of mitochondria in stem cell fate and aging[J]. Development, 2018, 145(8):dev143420.
[17]	GUO Y S, CHI X P, WANG Y F, et al. Mitochondria transfer enhances proliferation, migration, and osteogenic differentiation of bone marrow mesenchymal stem cell and promotes bone defect healing[J]. Stem Cell Res Ther, 2020, 11(1):245.
[18]	谌顺清, 梁伟, 张雪妹, 等. 骨碎补化学成分和药理作用研究进展[J]. 中国中药杂志, 2021, 46(11):2737-2745.CHEN S Q, LIANG W, ZHANG X M, et al. Research progress on chemical compositions and pharmacological action of Drynariae Rhizoma [J]. China Journal of Chinese Materia Medica, 2021, 46(11):2737-2745. (in Chinese)
[19]	黄敏玲, 卢赵琦, 申震, 等. 骨碎补总黄酮干预Notch信号通路影响骨重建过程中成血管-成骨耦联[J]. 中国组织工程研究, 2021, 25(32):5116-5122.HUANG M L, LU Z Q, SHEN Z, et al. Effects of total flavone of Rhizoma Drynariae on the coupling of angiogenesis and osteogenesis in bone remodeling through notch signaling pathway[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(32):5116-5122. (in Chinese)

Effects of Total Flavonoids of Drynariae Rhizoma on Osteogenic Differentiation Potential of Canine Bone Marrow Mesenchymal Stem Cells in Hypoxic Environment

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 19

Related Articles 1

Recommended Articles

Metrics

Comments