基于转录组测序研究健康三益菌对肉鸡脂质代谢的影响

doi:10.11843/j.issn.0366-6964.2022.04.015

摘要/Abstract

摘要： 旨在探讨健康三益菌调控肉鸡脂质代谢的主要基因和通路，阐明其影响脂质代谢的作用机理。本研究选取200只1日龄雄性黄麻肉鸡随机分为2组，每组5个重复，每个重复20只，对照组正常饮水，益生菌组在饮水中添加1%健康三益菌复合制剂(干酪乳杆菌∶嗜酸乳杆菌∶双歧杆菌=1∶1∶2)，试验期为42 d。测定肉鸡肉质品质，并对回肠进行转录组测序分析。结果表明，与对照组相比，添加健康三益菌组肉鸡腹脂率和剪切力有降低趋势，胸肌率和肌内脂肪有增加趋势(P>0.05)。转录组测序结果分析表明，与脂质代谢相关显著差异基因有12个，较对照组而言，益生菌组中LPL、PLIN1、GOS2、FABP4、RBP7、THRSP、FABP3、SCD、THEM4和CYP2C18表达量显著上调(P<0.05)，CYP7B1和GGT5表达量显著下调(P<0.05)；这些基因主要富集在对脂肪酸的反应、乳糜微粒重构、对脂质的反应、白脂肪细胞分化和长链脂肪酸结合等GO条目以及甾类激素生物合成、PPAR信号通路、花生四烯酸代谢、脂肪细胞中的脂肪分解等相关的重要通路。结果表明，健康三益菌可以通过调控脂肪沉积、转运、分解等相关基因的表达和相关通路来影响肉鸡脂质代谢，可能降低腹脂含量，促进肌内脂肪沉积。

关键词: 健康三益菌, 肉鸡, 脂质代谢, 转录组

Abstract: The study aimed to investigate the main genes and pathways of healthy tribeneficial bacteria regulating lipid metabolism in broilers, and to elucidate the mechanism of their effects on lipid metabolism. A total of 200 one-day-old male Jute broilers were randomly divided into 2 groups with 5 replicates per group and 20 broilers per replicate. The individuals in control group drank water normally, and the individuals in probiotic group drank water added with 1% healthy tribeneficial bacteria compound preparation (Lactobacillus casei∶Lactobacillus acidophilus∶Bifidobacterium=1∶1∶2). The experiment lasted for 42 days. The meat quality of broilers were measured and ileal transcriptome sequencing was performed. The results showed that, compared with the control group, the abdominal fat percentage and shear force of broilers supplemented with healthy tribeneficial bacteria decreased, and the breast muscle percentage and intramuscular fat increased (P>0.05). Transcriptome sequencing showed that there were 12 significantly differentially expressed genes related to lipid metabolism. Compared with the control group, the expressions levels of LPL, PLIN1, GOS2, FABP4, RBP7, THRSP, FABP3, SCD, THEM4 and CYP2C18 in probiotic group were significantly up-regulated (P<0.05), and the expressions levels of CYP7B1 and GGT5 were significantly down-regulated (P<0.05). These genes were mainly enriched in GO items such as response to fatty acids, chylomicron remodeling, response to lipid, white fat cell differentiation and long-chain fatty acid binding, as well as important pathways related to steroid hormone biosynthesis, PPAR signaling pathway, arachidonic acid metabolism and regulation of lipolysis in adipocytes. The results showed that healthy tribeneficial bacteria can affect lipid metabolism, reduce abdominal fat content and promote intramuscular fat deposition of broilers by regulating the related pathways and the expression of genes related to lipid deposition, transport and decomposition.

Key words: healthy tribeneficial bacteria, broilers, lipid metabolism, transcriptome

中图分类号:

S831.4

张利环, 贾浩, 王燕飞, 张若男, 刘璇. 基于转录组测序研究健康三益菌对肉鸡脂质代谢的影响[J]. 畜牧兽医学报, 2022, 53(4): 1154-1164.

ZHANG Lihuan, JIA Hao, WANG Yanfei, ZHANG Ruonan, LIU Xuan. Effects of Healthy Tribeneficial Bacteria on Lipid Metabolism in Broilers Based on Transcriptome Sequencing[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(4): 1154-1164.

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