免疫荧光检测绵羊体外受精胚胎Oct4、Cdx2表达研究

doi:10.11843/j.issn.0366-6964.2022.03.028

畜牧兽医学报 ›› 2022, Vol. 53 ›› Issue (3): 956-963.doi: 10.11843/j.issn.0366-6964.2022.03.028

免疫荧光检测绵羊体外受精胚胎Oct4、Cdx2表达研究

毛林军¹, 陈莹³, 雷熙¹, 彭新荣^2*, 齐·阿拉达尔^1*

1. 新疆农业大学动物科学学院, 乌鲁木齐 830052;
2. 新疆畜牧科学院生物技术研究所, 乌鲁木齐 830011;
3. 石河子大学动物科技学院, 石河子 832000

收稿日期:2021-05-24 出版日期:2022-03-23 发布日期:2022-03-31
通讯作者: 彭新荣,主要从事动物胚胎发育研究,E-mail:1069168161@qq.com;齐·阿拉达尔,主要从事动物遗传进化与功能研究,E-mail:157518003@qq.com
作者简介:毛林军(1994-),男,彝族,云南丽江人,硕士,主要从事动物遗传与育种研究,E-mail:2226843228@qq.com
基金资助:
国家自然科学基金地区基金项目(31760718)

Study on Expression of Oct4 and Cdx2 in in vitro Fertilization Embryos of Sheep by Immunofluorescence Detection

MAO Linjun¹, CHEN Ying³, LEI Xi¹, PENG Xinrong^2*, Qi Aladaer^1*

1. College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China;
2. Biotechnology Institute of Xinjiang Academy of Animal Science, Urumqi 830011, China;
3. College of Animal Science and Technology, Shihezi University, Shihezi 832000, China

Received:2021-05-24 Online:2022-03-23 Published:2022-03-31

摘要/Abstract

摘要： Oct4基因和Cdx2基因是附植前胚胎发育的重要调控基因,并最终调控了胎儿和胎盘的发育,也在胚胎妊娠识别和附植时调控了干扰素(interferon tau,IFNT)的表达。本研究通过体外成熟、体外受精和体外培养获得了不同发育时期的绵羊胚胎,应用免疫荧光染色探讨了Oct4在早期胚胎的表达规律,结果表明:受精卵和早期卵裂胚中未检测到Oct4蛋白;16~32细胞期桑葚胚部分细胞表达Oct4蛋白;Oct4与Cdx2基因在绵羊囊胚滋养层细胞中存在共表达。

关键词: 绵羊, Oct4, 囊胚, 滋养层, 共表达

Abstract: Oct4 and Cdx2 play important roles in the development of sheep pre-implantation embryos, which regulate the development of fetus and placenta. Oct4 and Cdx2 also regulate the expression of interferon-tau(IFNT), which plays a key role in embryo implantation and pregnancy recognization.In this study, embryos of different development stages were obtained by in vitro maturation,fertilization and cultured. Expression of Oct4 in the ovine embryos were analyzed using immunofluorescence staining. The results showed that expression of Oct4 protein was not detected in the zygotes and the early cleaved embryos. At the 16~32 morula cell stage, only a part of cells expressed Oct4 protein. Co-expression of Oct4 and Cdx2 was existed in the trophoblast of ovine blastocyst.

Key words: sheep, Oct4, blastocyst, trophoblast, co-expression

中图分类号:

S826.3

毛林军, 陈莹, 雷熙, 彭新荣, 齐·阿拉达尔. 免疫荧光检测绵羊体外受精胚胎Oct4、Cdx2表达研究[J]. 畜牧兽医学报, 2022, 53(3): 956-963.

MAO Linjun, CHEN Ying, LEI Xi, PENG Xinrong, Qi Aladaer. Study on Expression of Oct4 and Cdx2 in in vitro Fertilization Embryos of Sheep by Immunofluorescence Detection[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(3): 956-963.

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免疫荧光检测绵羊体外受精胚胎Oct4、Cdx2表达研究

Study on Expression of Oct4 and Cdx2 in in vitro Fertilization Embryos of Sheep by Immunofluorescence Detection

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