卵黄抗体对鸽毛滴虫与白色念珠菌感染的治疗效果

doi:10.11843/j.issn.0366-6964.2022.02.033

畜牧兽医学报 ›› 2022, Vol. 53 ›› Issue (2): 658-662.doi: 10.11843/j.issn.0366-6964.2022.02.033

• 研究简报 • 上一篇

卵黄抗体对鸽毛滴虫与白色念珠菌感染的治疗效果

王飞¹, 张禾¹, 蔡淯涵¹, 黄中佐¹, 严沛洁¹, 王李格¹, 孙鸿³, 李复煌^2*, 何诚^1*

1. 中国农业大学动物医学院, 北京 100193;
2. 北京市畜牧总站, 北京 100107;
3. 北京优帝鸽业有限公司, 北京 100188

收稿日期:2021-05-07 出版日期:2022-02-23 发布日期:2022-03-02
通讯作者: 何诚,主要从事人兽共患感染和免疫研究,E-mail:chenghe@cau.edu.cn;李复煌,主要从事家禽特色养殖生产关键技术研究,E-mail:lfh5118@126.com
作者简介:王飞(1998-),男,山西忻州人,硕士生,主要从事临床兽医感染和免疫研究,E-mail:398495247@qq.com
基金资助:
北京市大学生创新创业实践项目（Bj202010019287X）

The Therapeutic Effect of Yolk Antibody on the Infection of Trichomonas gallinae and Candida albicans

WANG Fei¹, ZHANG He¹, CAI Yuhan¹, HUANG Zhongzuo¹, YAN Peijie¹, WANG Lige¹, SUN Hong³, LI Fuhuang^2*, HE Cheng^1*

1. College of Veterinary Medicine, China Agricultural University, Beijing 100193, China;
2. Beijing Animal Husbandry Station, Beijing 100107, China;
3. Beijing Youdi Pigeon Industry Co., Ltd., Beijing 100188, China

Received:2021-05-07 Online:2022-02-23 Published:2022-03-02

摘要/Abstract

摘要： 旨在研究鸽毛滴虫与白色念珠菌二联卵黄抗体制备方法和评价其临床效果。利用临床分离的白色念珠菌与毛滴虫制备二联灭活疫苗，免疫产蛋母鸡获得高水平抗体，采取低温冻干技术制备成卵黄抗体粉。建立ELISA抗体检测法测定卵黄抗体水平，确保质量可控；以分离的肉鸽毛滴虫、白色念珠菌制备发病模型，评价卵黄抗体的保护效果。结果表明：二次免疫后，卵黄中毛滴虫抗体和白色念珠菌抗体OD值达到高峰，分别为0.90和0.66，抗体维持时间达到2个月。卵黄抗体按1 g·只^-1口服饲喂5 d后，肉鸽口腔和嗉囊病变评分与感染对照组相比显著降低（P<0.05），与甲硝唑、制霉菌素无差异；此外，毛滴虫、白色念珠菌病原载量极显著降低（P<0.01）。研制的鸽毛滴虫与白色念珠菌二联卵黄抗体可以替代甲硝唑和制霉菌素，为肉鸽的健康养殖提供一种新的抗菌素替代产品。

关键词: 肉鸽, 毛滴虫, 白色念珠菌, 卵黄抗体粉, 甲硝唑, 制霉菌素

Abstract: This study aimed to prepare the dual yolk antibody of Trichomonas gallinae and Candida albicans and study its therapeutic effect. Based on isolates of T. gallinae and C. albicans, we prepared a bivalent vaccine, harvested high-level antibody by inoculating to layers and produced into powders using freeze-dry system under low temperature. Moreover, antibody levels were determined to check quality control using in-house ELISA method. To test efficacy, pigeon models were developed by artificial infection with T. gallinae and C. albicans, respectively. The OD values of T. gallinae antibody and C. albicans antibody in the yolk reached their peaks after second immunization, which were 0.90 and 0.66, respectively, and the antibody maintenance time reached 2 months.In vivo study, both oral- and crop lesions score were reduced significantly(P<0.05)post feeding with 1 g of hyperimmune antibody powders per bird for 5 days and no significant difference was found compared to those pigeons treated with metronidazole and nystatin. Moreover, pathogen loads were reduced greatly compared to the control group(P<0.01). The hyperimmune antibody powder is a promising approach to replace metronidazole and nystatin, contributing to a novel solution for healthy development of pigeon industry.

Key words: meat pigeon, Trichomonas gallinae, Candida albicans, hyperimmune antibody powder, metronidazole, nystatin

中图分类号:

S858.395

王飞, 张禾, 蔡淯涵, 黄中佐, 严沛洁, 王李格, 孙鸿, 李复煌, 何诚. 卵黄抗体对鸽毛滴虫与白色念珠菌感染的治疗效果[J]. 畜牧兽医学报, 2022, 53(2): 658-662.

WANG Fei, ZHANG He, CAI Yuhan, HUANG Zhongzuo, YAN Peijie, WANG Lige, SUN Hong, LI Fuhuang, HE Cheng. The Therapeutic Effect of Yolk Antibody on the Infection of Trichomonas gallinae and Candida albicans[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 658-662.

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卵黄抗体对鸽毛滴虫与白色念珠菌感染的治疗效果

The Therapeutic Effect of Yolk Antibody on the Infection of Trichomonas gallinae and Candida albicans

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