白蚁肠道纤维素降解菌的分离和鉴定

doi:10.11843/j.issn.0366-6964.2019.09.026

畜牧兽医学报 ›› 2019, Vol. 50 ›› Issue (9): 1951-1954.doi: 10.11843/j.issn.0366-6964.2019.09.026

• 研究简报 • 上一篇

白蚁肠道纤维素降解菌的分离和鉴定

马玉俊¹, 张凯¹, 王磊¹, 张康¹, 张宏², 王学智¹, 李杰¹, 梁子敬¹, 张景艳^1*, 李建喜^1*

1. 中国农业科学院兰州畜牧与兽药研究所, 中兽医研究实验室, 兰州 730050;
2. 甘肃省动物卫生监督所, 兰州 730000

收稿日期:2019-01-22 出版日期:2019-09-23 发布日期:2019-09-23
通讯作者: 张景艳,主要从事中兽医与免疫研究,E-mail:zwzh1223@126.com;李建喜,主要从事兽医药理毒理、奶牛疾病防制研究,E-mail:lzjianxil@163.com
作者简介:马玉俊(1993-),男,甘肃临夏人,硕士生,主要从事兽医内科学研究,E-mail:2451276640@qq.com
基金资助:
国家自然科学基金（31602101）；甘肃省农业生物技术研究与应用开发项目（GNSW-2016-12）；甘肃省科技支撑计划项目（1604NKCA069-03）

Isolation and Identification of Cellulose-degrading Bacteria in Termite Gut

MA Yujun¹, ZHANG Kai¹, WANG Lei¹, ZHANG Kang¹, ZHANG Hong², WANG Xuezhi¹, LI Jie¹, LIANG Zijing¹, ZHANG Jingyan^1*, LI Jianxi^1*

1. Chinese Veterinary Research Laboratory, Lanzhou Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China;
2. Gansu Animal Health Inspection Institute, Lanzhou 730000, China

Received:2019-01-22 Online:2019-09-23 Published:2019-09-23

摘要/Abstract

摘要： 研究白蚁肠道产纤维素酶菌株，寻找新的高产纤维素酶菌株。采用培养基分离方法，纯化白蚁肠道产纤维素酶菌株，比较菌株3种纤维素酶活性，并进行16S rDNA及生化鉴定。筛选出10株产纤维素酶菌株（CX1~10），其中CX10菌株水解圈直径比值最大为2.78。测得48 h内CX10菌株滤纸酶（FPA）活力最高，为49.5 IU·mL^-1。CX9的纤维素外切酶（CX）活力最高，为25.9 U·mL^-1。CX8、CX9和CX10的纤维素内切酶（CMCase）活力分别为67.8、70.4和95.0 IU·mL^-1。经鉴定白蚁肠道产纤维素酶菌株为芽胞杆菌属（Bacillus）、枸橼酸杆菌属（Citrobacter）及沙雷菌属（Serratia）。本研究从低等白蚁肠道成功分离出10株菌，其中CX10纤维素综合酶活力最高，为坚强芽胞杆菌（Bacillus firms）。

Abstract: In order to seek high cellulase-producing strains, the termites intestinal flora was studied. The bacteria in intestinal tract were isolated by the method of medium separation under aerobic and anaerobic conditions. Three cellulase activities in the screened strains were analyzed at 48 h, and the strains were identified by gene sequencing and biochemical analysis. Ten cellulase-producing bacteria strains were screened (CX1-10), and the ratio of the diameter of hydrolysis zone was the largest (2.78) in CX10. Filter Paper Enzyme (FPA) in CX10 had the highest activity, reaching 49.5 IU·mL^-1. Cellulose Endonuclease in CX9 had the highest activity, reaching 25.9 IU·mL^-1. Cellulose endoglucanases in CX8, CX9 and CX10 were 67.8, 70.4 and 95.0 IU·mL^-1, respectively. All of the screened strains were identified as Bacillus, Citrobacter and Serratia. In this study, 10 bacteria were successfully isolated from the intestines of lower termites, among which the CX10 synthesize cellulase activity was the highest, which was identified as Bacillus firms.

中图分类号:

X172

马玉俊, 张凯, 王磊, 张康, 张宏, 王学智, 李杰, 梁子敬, 张景艳, 李建喜. 白蚁肠道纤维素降解菌的分离和鉴定[J]. 畜牧兽医学报, 2019, 50(9): 1951-1954.

MA Yujun, ZHANG Kai, WANG Lei, ZHANG Kang, ZHANG Hong, WANG Xuezhi, LI Jie, LIANG Zijing, ZHANG Jingyan, LI Jianxi. Isolation and Identification of Cellulose-degrading Bacteria in Termite Gut[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(9): 1951-1954.

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白蚁肠道纤维素降解菌的分离和鉴定

Isolation and Identification of Cellulose-degrading Bacteria in Termite Gut

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