细粒棘球绦虫原头蚴lncRNA表达谱分析

doi:10.11843/j.issn.0366-6964.2019.09.014

畜牧兽医学报 ›› 2019, Vol. 50 ›› Issue (9): 1864-1873.doi: 10.11843/j.issn.0366-6964.2019.09.014

细粒棘球绦虫原头蚴lncRNA表达谱分析

王正荣¹, 张艳艳¹, 马勋², 刘乙¹, 徐梦飞^1,2, 孟季蒙¹, 薄新文^1*

1. 新疆农垦科学院畜牧兽医研究所, 省部共建绵羊遗传改良与健康养殖国家重点实验室, 石河子 832000;
2. 石河子大学动物科技学院, 石河子 832000

收稿日期:2019-04-10 出版日期:2019-09-23 发布日期:2019-09-23
通讯作者: 薄新文,主要从事家畜寄生虫学与寄生虫病研究,E-mail:xinwen_bo@126.com
作者简介:王正荣(1984-),男,甘肃定西人,助理研究员,硕士,主要从事家畜寄生虫学与寄生虫病研究,Tel:0993-6683358,E-mail:wzrtiger@sina.com
基金资助:
国家自然科学基金（31860701）；国家重点基础研究发展计划（973计划）（2015CB150300）；新疆生产建设兵团国际科技合作（2017BC003）

lncRNA Profile Analyses of the Protoscoleces in Echinococcus granulosus

WANG Zhengrong¹, ZHANG Yanyan¹, MA Xun², LIU Yi¹, XU Mengfei^1,2, MENG Jimeng¹, BO Xinwen^1*

1. State Key Labaratory of Sheep Genetic Improvement and Healthy Production/Institute of Animal Husbandry and Veterinary, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China;
2. College of Animal Science and Technology, Shehezi University, Shihezi 832000, China

Received:2019-04-10 Online:2019-09-23 Published:2019-09-23

摘要/Abstract

摘要： 本研究旨在解析lncRNA在细粒棘球绦虫原头蚴中的表达谱特性，为进一步揭示lncRNA在细粒棘球绦虫原头蚴发育中的作用及调控机制提供理论依据。从患病绵羊肝无菌采集细粒棘球绦虫原头蚴，提取总RNA，构建原头蚴lncRNA文库，利用RNA sequencing技术进行测序分析。结果表明，首次从细粒棘球绦虫原头蚴中鉴定出609个新的lncRNA分子。其中intergenic lncRNA有431个，intronic lncRNA有11个，anti-sense lncRNA有68个，sence lncRNA有99个。GO分析表明，其表达量前200的lncRNA分子主要与MAP激酶的负调控、SMC结合复合物、IMP的生物合成以及乙酰转移酶激活剂活性相关。KEGG分析结果表明，其表达量前200的lncRNA分子主要与细胞凋亡、萜类骨架生物合成、碳代谢以及背腹轴形成相关。cis分析结果表明，在这609个新的lncRNA分子中，与虫体Wnt信号通路、Hedgehog信号通路、NF-κB信号通路、cAMP信号通路、Notch信号通路、胆酸盐信号通路以及背腹轴信号通路相关的lncRNA分子分别有79、31、10、128、43、84和73个。qRT-PCR的验证结果表明，表达量前10的lncRNA分子与测序结果一致。RNA荧光探针原位杂交结果表明，高表达的lncRNA分子在细粒棘球绦虫原头蚴吸盘以及表皮均有表达。综上所述，本研究首次解析了细粒棘球绦虫原头蚴中lncRNA的表达谱特性，丰富了细粒棘球绦虫lncRNA的数据，为进一步解析lncRNA在细粒棘球绦虫原头蚴发育中的作用奠定了基础。

Abstract: The purpose of this study was to analyze the expression profile characteristics of lncRNA of the protoscoleces in Echinococcus granulosus and to provide theoretical evidence for further revealing the role of lncRNA in the development of the protoscoleces. The protoscoleces were collected from the infected sheep liver under sterile condition, and the total RNA was extracted. Then the lncRNA Library of the protoscoleces was constructed and sequenced by RNA sequencing technology. The results showed that 609 novel lncRNA molecules were identified from the protoscoleces for the first time. Among them, there are 431 intergenic lncRNAs, 11 intronic lncRNAs, 68 anti-sense lncRNAs, and 99 sence lncRNAs. GO analysis indicated that the top 200 lncRNA molecules were mainly associated with negative regulation of MAP kinase, SMC binding complex, IMP biosynthesis, and acetyltransferase activator activity. The results of KEGG analysis indicated that the top 200 lncRNA molecules were mainly associated with apoptosis, steroid skeleton biosynthesis, carbon metabolism, and dorsal-ventral axis formation. The results of cis analysis indicated that in these 609 novel lncRNA molecules, there are 79, 31, 10, 128, 43, 84 and 73 lncRNA moleculess potential interplayed with the Wnt signaling pathway, Hedgehog signaling pathway, NF-κB signaling pathway, cAMP signaling pathway, Notch signaling pathway, bile-salt signaling pathway, and dorsal-ventral axis. The results of qRT-PCR showed that the top 10 lncRNA molecules were consistent with the sequencing results. The results of whole-mount in situ hybridization showed that the highly expressed lncRNA molecules were expressed in the sucker, epidermis and disperse cells of the protoscoleces. To sum up, a large number of novel lncRNA molecules were found in the E. granulosus in this study, which enriched the data of lncRNA of the protoscoleces, and laid a foundation for further analysis of the role of lncRNA in the development of protoscoleces in E. granulosus.

中图分类号:

S852.734

王正荣, 张艳艳, 马勋, 刘乙, 徐梦飞, 孟季蒙, 薄新文. 细粒棘球绦虫原头蚴lncRNA表达谱分析[J]. 畜牧兽医学报, 2019, 50(9): 1864-1873.

WANG Zhengrong, ZHANG Yanyan, MA Xun, LIU Yi, XU Mengfei, MENG Jimeng, BO Xinwen. lncRNA Profile Analyses of the Protoscoleces in Echinococcus granulosus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(9): 1864-1873.

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细粒棘球绦虫原头蚴lncRNA表达谱分析

lncRNA Profile Analyses of the Protoscoleces in Echinococcus granulosus

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