褪黑激素对内蒙古绒山羊皮肤中let-7家族及其靶基因的影响

doi:10.11843/j.issn.0366-6964.2019.07.006

畜牧兽医学报 ›› 2019, Vol. 50 ›› Issue (7): 1367-1379.doi: 10.11843/j.issn.0366-6964.2019.07.006

褪黑激素对内蒙古绒山羊皮肤中let-7家族及其靶基因的影响

王建蒙^1,2, 付绍印^3,4, 丽春⁵, 白雪^1,2, 刘斌^2,6, 季小阳^1,2, 刘永斌^3*, 张文广^1,2,4*

1. 内蒙古农业大学动物科学学院动物遗传育种与繁殖自治区重点实验室, 呼和浩特 010018;
2. 内蒙古自治区农业基因组大数据工程研究中心, 呼和浩特 010018;
3. 内蒙古自治区农牧业科学院, 呼和浩特 010031;
4. 内蒙古ATCG生物信息研究所, 呼和浩特 010020;
5. 内蒙古民族大学动物科学技术学院, 通辽 028000;
6. 内蒙古犇牛科技有限公司, 呼和浩特 010018

收稿日期:2018-11-26 出版日期:2019-07-23 发布日期:2019-07-23
通讯作者: 刘永斌,主要从事分子遗传与动物遗传育种研究,E-mail:ybliu117@126.com;张文广,主要从事数量基因组学暨性状遗传机理研究,E-mail:atcgnmbi@aliyun.com
作者简介:王建蒙(1993-),男,内蒙古赤峰人,硕士生,主要从事动物遗传育种与繁殖研究,E-mail:wangjianmeng100861@163.com
基金资助:
国家自然科学基金（31260538；31402052）：内蒙古民族大学科学研究项目（BS308）；内蒙古自然科学基金（2014BS0313；2017MS0342）；内蒙古自治区高等学校青年科技英才项目（NJYT-17-B31）

Effects of Melatonin on let-7 Family and Its Target Genes in Skin of Inner Mongolian Cashmere Goats

WANG Jianmeng^1,2, FU Shaoyin^3,4, LI Chun⁵, BAI Xue^1,2, LIU Bin^2,6, JI Xiaoyang^1,2, LIU Yongbin^3*, ZHANG Wenguang^1,2,4*

1. Inner Mongolia Key Laboratory of Animal Genetics, Breeding and Reproduction, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Inner Mongolia Engineering Research Center of Genomic Big Data for Agriculture, Hohhot 010018, China;
3. Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, China;
4. Inner Mongolia ATCG Institute of Bioinformatics, Hohhot 010020, China;
5. College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao 028000, China;
6. Inner Mongolia BioNew Technology Co. Ltd., Hohhot 010018, China

Received:2018-11-26 Online:2019-07-23 Published:2019-07-23
Supported by:

摘要/Abstract

摘要： 旨在探索褪黑激素（melatonin，MT）对let-7家族成员及其靶基因在内蒙古绒山羊皮肤周期性表达的影响。本研究选取内蒙古绒山羊罕山型个体6只，分为埋植组（皮下埋植MT，n=3）和对照组（不埋植MT， n=3），连续12个月采集绒山羊个体皮肤组织为试验材料，利用qRT-PCR技术检测皮肤组织中11个let-7家族成员的表达变化，结合RNA-Seq技术分析let-7家族靶基因的表达变化。结果表明：1）在整个皮肤毛囊周期内，MT上调let-7b、let-7e、let-7a-3p的表达，下调let-7f、let-7g、let-7c、let-7a-5p、let-7d、miR-98的表达。2）在4月份，MT上调“let-7g、let-7i、miR-98”的表达，下调PTGS2、PLCG1等靶基因的表达；下调“let-7a-5p、let-7b、let-7c”的表达，上调TGIF2、RBL1等靶基因的表达。3）在6月份，MT下调“let-7g、let-7i、miR-98”的表达，上调PTGS2、PLCG1、SRC等靶基因的表达；上调“let-7a-5p、let-7b、let-7c”的表达，下调GDF5、TGIF2、RBL1等靶基因的表达。4） let-7家族成员的靶基因主要参与VEGF、TGF-β、Oxytocin和NF-kappa B信号通路，在细胞过程、激素调节和转录因子调控方面发挥重要作用。综上表明，MT可能通过改变let-7家族重要成员的表达水平，影响相应靶基因的表达，进而在皮肤毛囊的周期性生长过程中发挥重要作用，研究结果为进一步揭示MT介导miRNAs影响羊绒生长的分子机理提供了理论依据。

Abstract: The study aimed to explore the effects of melatonin on the periodic expression of let-7 family and its target genes in skin of Inner Mongolia Cashmere goat. In this study, six Inner Mongolia Hanshan Cashmere goats were selected and divided equally and randomly into 2 groups, melatonin implantation group and non-implantation group. Taking the skin tissue of the goats as experimental material in every months of the year. The expression changes of 11 members in let-7 family were quantified by reverse transcription-real time polymerase chain reaction (qRT-PCR) in the skin tissue. The expression changes of the target genes of let-7 family were analyzed using the RNA-Seq technique. The results showed that:1) During the whole hair follicle cycle, melatonin up-regulated the expression of let-7b, let-7e and let-7a-3p, down-regulated the expression of let-7f, let-7g, let-7c, let-7a-5p, let-7d and miR-98. 2) In April, melatonin up-regulated the expression of "let-7g, let-7i and miR-98", down-regulated the expression of target genes such as PTGS2 and PLCG1. It down-regulated the expression of "let-7a-5p, let-7b and let-7c", up-regulated the expression of target genes such as TGIF2 and RBL1. 3) In June, melatonin down-regulated the expression of "let-7g, let-7i and miR-98", up-regulated the expression of target genes such as PTGS2, PLCG1 and SRC; It up-regulated the expression of "let-7a-5p, let-7b and let-7c", down-regulated the expression of target genes such as GDF5, TGIF2 and RBL1. 4) The target genes of let-7 family were mainly involved in VEGF, TGF-β, Oxytocin and NF-kappa B signaling pathways, and played an important role in cell processes, hormone regulation and regulation of transcription factors. In summary, MT may affect the expression of the corresponding target genes by changing the expression level of members in the let-7 family. It plays an important role in the periodic growth of skin hair follicles. These results provided a theoretical basis for further revealing the molecular mechanism of melatonin mediating miRNAs to affect cashmere growth.

中图分类号:

S826.2

王建蒙, 付绍印, 丽春, 白雪, 刘斌, 季小阳, 刘永斌, 张文广. 褪黑激素对内蒙古绒山羊皮肤中let-7家族及其靶基因的影响[J]. 畜牧兽医学报, 2019, 50(7): 1367-1379.

WANG Jianmeng, FU Shaoyin, LI Chun, BAI Xue, LIU Bin, JI Xiaoyang, LIU Yongbin, ZHANG Wenguang. Effects of Melatonin on let-7 Family and Its Target Genes in Skin of Inner Mongolian Cashmere Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(7): 1367-1379.

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褪黑激素对内蒙古绒山羊皮肤中let-7家族及其靶基因的影响

Effects of Melatonin on let-7 Family and Its Target Genes in Skin of Inner Mongolian Cashmere Goats

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