沙冬青种子总黄酮对免疫抑制小鼠免疫功能及胸腺和脾超微结构的影响

doi:10.11843/j.issn.0366-6964.2018.08.021

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (8): 1752-1760.doi: 10.11843/j.issn.0366-6964.2018.08.021

沙冬青种子总黄酮对免疫抑制小鼠免疫功能及胸腺和脾超微结构的影响

黄伟宽¹, 刘瑞菊², 贾宁^1*, 方梅¹, 陶波^1,3, 张嘉男¹, 梁鹏飞¹, 邓康¹

1. 甘肃农业大学动物医学院, 兰州 730070;
2. 山东省寿光市营里镇畜牧兽医管理站, 寿光 262700;
3. 临夏现代职业学院, 临夏 731100

收稿日期:2018-01-08 出版日期:2018-08-23 发布日期:2018-08-23
通讯作者: 贾宁(1963-),男,教授,博士,主要从事兽医病理学与分子病理学研究,E-mail:1963jianing@163.com
作者简介:黄伟宽(1994-),男,黎族,海南保亭人,硕士生,主要从事兽医病理学研究,E-mail:569590370@qq.com
基金资助:
国家自然科学基金（31560686）

The Effect of Total Flavonoids from Ammopiptanthus mongolicus on the Immune Function and Ultrastructure of Thymus and Spleen in Immunosuppressed Mice

HUANG Wei-kuan¹, LIU Rui-ju², JIA Ning^1*, FANG Mei¹, TAO Bo^1,3, ZHANG Jia-nan¹, LIANG Peng-fei¹, DENG Kang¹

1. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China;
2. Animal Husbandry and Veterinary Station of Shouguang, Shouguang 262700, China;
3. Linxia Modern Career Academy, Linxia 731100, China

Received:2018-01-08 Online:2018-08-23 Published:2018-08-23

摘要/Abstract

摘要：

旨在深入探讨沙冬青种子总黄酮对免疫抑制小鼠的免疫功能及胸腺和脾超微结构的影响。将100只昆明种小鼠随机分为5组：空白对照组、免疫抑制组、试验Ⅰ组、试验Ⅱ组和试验Ⅲ组，每组20只（雌雄各半），采用灌胃法给药，前7 d，免疫抑制组、试验Ⅰ组、试验Ⅱ组与试验Ⅲ组小鼠均灌胃0.6 mL环磷酰胺溶液；后21 d，试验Ⅰ组、试验Ⅱ组、试验Ⅲ组分别灌胃0.6 mL黄酮剂量为50、150、250 mg·（kg·d）^-1的总黄酮溶液，未明确写出的组别和时间点在上述给药时段相应灌胃0.6 mL生理盐水。试验28 d后，分别测定碳粒廓清指数和脏器指数（胸腺、脾）及小鼠血清IL-2、IL-4含量。同时，采用扫描电镜观察脾和胸腺结构。结果表明，灌胃环磷酰胺可复制小鼠免疫抑制模型。不同剂量的沙冬青种子总黄酮能不同程度提高免疫抑制小鼠胸腺和脾指数、碳粒廓清指数、吞噬指数以及血清IL-2和IL-4含量，尤其试验Ⅱ组，总黄酮灌胃剂量为150 mg·（kg·d）^-1，其效果最为突出，与免疫抑制组比较均差异极显著（P<0.01）。电镜观察显示，沙冬青种子总黄酮不同剂量试验组小鼠胸腺、脾中成熟淋巴细胞数量与免疫抑制组小鼠比较均不同程度增多，尤其试验Ⅱ组中成熟淋巴细胞数量增多最明显（P<0.01），且淋巴细胞排列紧密，与周围间质间隙明显减少。上述结果提示，沙冬青种子总黄酮可通过促进免疫器官淋巴细胞的增殖和成熟，改善和修复受损免疫器官组织结构，从而达到改善和增强免疫抑制小鼠免疫功能的作用，且在一定范围内具有剂量依赖效应。

Abstract:

To investigate the effect of total flavonoids from the seeds of Ammopiptanthus mongolicus on the immune function and the ultrastructure of thymus and spleen in immunosuppressed mice,100 Kunming mice were randomly divided into five groups(n=20,half male and half female):normal control group, immunosuppression group (cyclophosphamide induced), test group (cyclophosphamide induced, total flavonoids treated) Ⅰ, test group Ⅱ, and test group Ⅲ, and using intragastric administration of medicine to mice. After 28 days, carbon clearance index, thymus index, spleen index, and the content of IL-2, IL-4 in serum were detected. At the same time, the ultrastructure of thymus and spleen were observed through scanning electron microscope. The results showed that the immunosuppressed mice were copied successfully with cyclophosphamide, and the total flavonoids from the seeds of Ammopiptanthus mongolicus could increase the carbon clearance index, thymus index, and spleen index, the content of IL-2 and IL-4 in serum in immunosuppressed mice. The difference were significantly compared with the immunosuppression group, especially in test group Ⅱ[150 mg·(kg·d)^-1total flavonoids] (P<0.01). Compared with the immunosuppression group, the number of mature lymphocytes in immunosuppressed mice thymus and spleen increased significantly in the total flavonoid-treated groups and the lymphocytes connected closely, especially in test groupⅡ[150 mg·(kg·d)^-1total flavonoids] (P<0.01). The results suggest that the total flavonoids from the seeds of Ammopiptanthus mongolicus can reverse and repair obviously the immune function of the immunosuppressed mice and promote obviously the proliferation and activation of lymphocytes,further more, it has does-dependent relationship with the total flavonoids in a certain extent.

中图分类号:

R282.71

黄伟宽, 刘瑞菊, 贾宁, 方梅, 陶波, 张嘉男, 梁鹏飞, 邓康. 沙冬青种子总黄酮对免疫抑制小鼠免疫功能及胸腺和脾超微结构的影响[J]. 畜牧兽医学报, 2018, 49(8): 1752-1760.

HUANG Wei-kuan, LIU Rui-ju, JIA Ning, FANG Mei, TAO Bo, ZHANG Jia-nan, LIANG Peng-fei, DENG Kang. The Effect of Total Flavonoids from Ammopiptanthus mongolicus on the Immune Function and Ultrastructure of Thymus and Spleen in Immunosuppressed Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(8): 1752-1760.

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参考文献

[1] 钟建青, 李波, 贾琦, 等. 天然黄酮类化合物及其衍生物的构效关系研究进展[J]. 药学学报, 2011, 46(6):622-630.
ZHONG J Q, LI B, JIA Q, et al. Advances in the structure-activity relationship study of natural flavonoids and its derivatives[J]. Acta Pharmaceutica Sinica, 2011, 46(6):622-630. (in Chinese)
[2] 张玉清, 王帅, 景娇, 等. 5种中药总黄酮成分体外抗病毒作用观察[J]. 南京农业大学学报, 2012, 35(4):105-109.
ZHANG Y Q, WANG S, JING J, et al. Anti-virus function of 5 kinds of total flavone ingredients in vitro[J]. Journal of Nanjing Agricultural University, 2012, 35(4):105-109. (in Chinese)
[3] HEIM K E, TAGLIAFERRO A R, BOBILYA D J. Flavonoid antioxidants:Chemistry, metabolism and structure-activity relationships[J]. J Nutr Biochem, 2002, 13(10):572-584.
[4] 陈慧, 宁明刚, 刘宇, 等. 沙冬青种子总黄酮对小鼠急性毒性作用研究[J]. 甘肃农业大学学报, 2013, 48(3):14-18.
CHEN H, NING M G, LIU Y, et al. Acute toxicity of the total flavonoid from seeds of Ammopiptanthus mongolicus on mice[J]. Journal of Gansu Agricultural University, 2013, 48(3):14-18. (in Chinese)
[5] 周建胜, 陈慧, 方梅, 等. 沙冬青种子总黄酮抗病毒活性的研究[J]. 中国兽医科学, 2013, 43(4):425-429.
ZHOU J S, CHEN H, FANG M, et al. Study on inhibitory effect of the total flavonoids from seeds of Ammopiptanthus on bovine viral diarrhea virus[J]. Chinese Veterinary Science, 2013, 43(4):425-429. (in Chinese)
[6] 陶波, 方梅, 张嘉男, 等. 沙冬青种子总黄酮测定方法及纯化工艺研究[J]. 生物技术通报, 2017, 33(5):63-70.
TAO B, FANG M, ZHANG J N, et al. Determination and purification of total flavonoids from seeds of Ammopiptanthus mongolica[J]. Biotechnology Bulletin, 2017, 33(5):63-70. (in Chinese)
[7] CHEN G S, QI H Z. Effect of Kupffer cells on immune tolerance in liver transplantation[J]. Asian Pac J Trop Med, 2012, 5(12):970-972.
[8] 王洪武, 贾亮亮, 徐媛青, 等. 淫羊藿总黄酮对环磷酰胺致免疫低下小鼠的免疫调节作用[J]. 天津医药, 2010, 38(12):1068-1071.
WANG H W, JIA L L, XU Y Q, et al. Immunoregulatory effects of total flavones of epimedium on immunosuppression mice[J]. Tianjin Medical Journal, 2010, 38(12):1068-1071. (in Chinese)
[9] 靳二辉, 陈耀星, 周金星, 等. 黄芪、枸杞、金银花等中草药复方制剂对肉鸡免疫器官发育及免疫功能的影响[J]. 畜牧兽医学报, 2017, 48(6):1128-1139.
JIN E H, CHEN Y X, ZHOU J X, et al. The effect of compound Chinese herbal medicine preparation of astragalus, wolfberry and honeysuckle flower on immune organ development and immune functions of broiler[J]. Acta Veterinaria et Zootechnica Sinica, 2017, 48(6):1128-1139. (in Chinese)
[10] 孙晓雨, 崔子寅, 张明亮, 等. 枸杞多糖和茯苓多糖对免疫抑制小鼠免疫增强及对肠道黏膜的免疫调节作用[J]. 中国兽医学报, 2015, 35(3):450-455.
SUN X Y, CUI Z Y, ZHANG M L, et al. Effect of Lycium barbarum polysaccharides and Pachymaran on immune function and intestinal mucosal system in immunosuppressive mice[J]. Chinese Journal of Veterinary Science, 2015, 35(3):450-455. (in Chinese)
[11] 王占彬, 王亚锴, 赵德明, 等. 毛泡桐花黄酮对小鼠脾和胸腺超微结构及免疫功能的影响[J]. 畜牧兽医学报, 2013, 44(6):952-959.
WANG Z B, WANG Y K, ZHAO D M, et al. The effect of flavonoids from Paulownia tomentosa flowers on the ultrastructure and immune function of spleen and thymus in mice[J]. Acta Veterinaria et Zootechnica Sinica, 2013, 44(6):952-959. (in Chinese)
[12] 黄伟, 陈绍红, 刘铀, 等. 构树总黄酮对免疫抑制小鼠免疫功能的影响[J]. 中国现代应用药学, 2017, 34(1):8-11.
HUANG W, CHEN S H, LIU Y, et al. Effects of total flavonoids of Broussonetia papyrifera on immunosuppressed mouse[J]. Chinese Journal of Modern Applied Pharmacy, 2017, 34(1):8-11. (in Chinese)
[13] 刘哲慧, 张琳. 水芹总黄酮对免疫抑制小鼠免疫功能的影响[J]. 中国中医药科技, 2016, 23(4):423-425.
LIU Z H, ZHANG L. Effects of total flavone extract from Shuiqin (Oenanthe Javanica) on immune function of immunosuppression mice[J]. Chinese Journal of Traditional Medical Science and Technology, 2016, 23(4):423-425. (in Chinese)
[14] CORBIN E, VICENTE J, MARTIN-HERNANDO M P, et al. Spleen mass as a measure of immune strength in mammals[J]. Mamm Rev, 2008, 38(1):108-115.
[15] WANG X. Research on effect of ginkgo aglucone flavone to human body organs and immune function[J]. Pak J Pharm Sci, 2014, 27(4S1):1099-1102.
[16] 邓桦, 杨鸿, 张兆, 等. 玉屏风复合多糖对环磷酰胺免疫抑制小鼠的恢复作用[J]. 中国兽医学报, 2014, 34(2):288-292.
DENG Y, YANG H, ZHANG Z, et al. Immune restorative effect of YPF-P on immunosuppressive mice influenced by cyclophosphamid[J]. Chinese Journal of Veterinary Science, 2014, 34(2):288-292. (in Chinese)
[17] 王勇, 司丽芳, 李相能, 等. 银杏叶提取物增强大鼠脾脏和胸腺的免疫功能[J]. 细胞与分子免疫学杂志, 2015, 31(6):792-795, 799.
WANG Y, SI L F, LI X N, et al. Ginkgo biloba extract enhances the immune function of spleen and thymus in SD rats[J]. Chinese Journal of Cellular and Molecular Immunology, 2015, 31(6):792-795, 799. (in Chinese)
[18] FAN Y P, MA L, ZHANG W M, et al. Microemulsion can improve the immune-enhancing activity of propolis flavonoid on immunosuppression and immune response[J]. Int J Biol Macromol, 2014, 63:126-132.
[19] SOBRAL F, CALHELHA R C, BARROS L, et al. Flavonoid composition and antitumor activity of bee bread collected in Northeast Portugal[J]. Molecules, 2017, 22(2):248.
[20] OKOYE F B C, ODIMEGWU D C, NWORU C S, et al. Modulation of intracellular expression of IFNγ and IL-2 in culture of splenic T lymphocytes by some flavonoid glycosides of Alchornea floribunda[J]. Pharm Biol, 2016, 54(9):1873-1880.
[21] PELUSO I, MIGLIO C, MORABITO G, et al. Flavonoids and immune function in human:A systematic review[J]. Crit Rev Food Sci Nutr, 2015, 55(3):383-395.

沙冬青种子总黄酮对免疫抑制小鼠免疫功能及胸腺和脾超微结构的影响

The Effect of Total Flavonoids from Ammopiptanthus mongolicus on the Immune Function and Ultrastructure of Thymus and Spleen in Immunosuppressed Mice

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