水牛STAT1基因多态与产奶性状关联分析

doi:10.11843/j.issn.0366-6964.2016.01.007

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (1): 48-54.doi: 10.11843/j.issn.0366-6964.2016.01.007

水牛STAT1基因多态与产奶性状关联分析

邓廷贤，庞春英^*，朱鹏，段安琴，陆杏蓉，杨炳壮，梁贤威^*

（中国农业科学院广西水牛研究所，农业部(广西)水牛遗传繁育重点实验室，南宁530001）

收稿日期:2015-01-19 出版日期:2016-01-23 发布日期:2016-01-23
通讯作者: 梁贤威，研究员，主要从事水牛繁殖与营养研究，E-mail：liangbri@126.com；庞春英，副研究员，主要从事水牛遗传育种研究，E-mail：pangcy800@163.com
作者简介:邓廷贤(1983-)，男，湖南郴州人，硕士，助理研究员，主要从事水牛分子遗传学研究，E-mail：dtx282000@163.com
基金资助:
国家国际科技合作专项(2014DFA31970)；桂渔牧科(1304512)

Association between Polymorphism of STAT1 Gene and Milk Production Traits in Buffalo (Bubalus bubalis)

DENG Ting-xian，PANG Chun-ying^*，ZHU Peng，DUAN An-qin，LU Xing-rong，YANG Bing-zhuang，LIANG Xian-wei^*

（Key Laboratory of Buffalo Genetics，Breeding and Reproduction Technology，Ministry of Agriculture and Guangxi，Buffalo Research Institute，Chinese Academy of Agricultural Sciences，Nanning 530001，China）

Received:2015-01-19 Online:2016-01-23 Published:2016-01-23

摘要/Abstract

摘要：

本研究旨在检测水牛STAT1基因的单核苷酸多态性（SNP），探究中国水牛多态性位点的群体遗传特征，寻找与产奶性状相关的分子标记。采用PCR和测序法筛选水牛STAT1基因序列的SNPs，以357头水牛为研究对象，采用基质辅助激光解吸电离飞行时间质谱法（MALDI-TOF-MS）对群体进行了基因型检测，运用SAS（9.3）软件一般线性模型（GLM）对STAT1基因型与产奶性状的关联程度进行统计分析。结果表明，在水牛STAT1基因中检测出3个突变位点，分别位于5′UTR（g.68G>A）、外显子10（g.986 G>T）和3′UTR（g.2881 C>A）。关联分析结果显示，水牛STAT1基因g.986G>T位点与305天产奶量和乳脂率显著关联（P<0.05）。Bonferroni t 检验多重比较结果显示，基因型TT和GG个体的305天产奶量显著高于GT基因型（P<0.05），基因型TT个体的乳脂率显著高于GG和GT基因型（P<0.05），且305天的产奶量随着胎次的增加呈现上升趋势，直到第3或4胎次时达到最大值，表明胎次是影响水牛产奶量的重要环境因素之一。本研究表明，水牛STAT1基因的g.986G>T位点可能是影响其产奶性状的候选分子遗传标记，为今后建立中国水牛标记辅助选择育种研究奠定基础。

Abstract:

This study aimed to identify single nucleotide polymorphism (SNP) of the buffalo STAT1 gene，investigate population genetics characters of SNPs and find molecular markers for milk production traits in Chinese buffaloes.The polymorphisms of STAT1 gene were detected by PCR and sequencing methods.Population genetic polymorphisms for 357 buffaloes were genotyped by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technology，and the associations analysis between genotypes and milk production traits was performed by the SAS (9.3) software with generalized linear model.The results showed that the total of 3 SNPs were detected on the 5′UTR，exon10 and 3′UTR in STAT1 gene，namely g.68G>A，g.986 G>T and g.2881 C>A，respectively.Association analysis indicated that 305-day milk yield and fat percentage were significantly associated with the g.986 G>T of STAT1 gene (P<0.05).The results of Bonferroni t-test revealed that 305-day milk yield of individuals with TT and GG genotypes were significantly higher than those with GT genotypes (P<0.05)，and fat percentage of individuals with TT genotype were significantly higher than those with GG and GT genotypes (P<0.05).And the 305-day milk yield tended to increase from first to later parities，reaching the maximum at the 3rd or 4th calving，indicating that parity was one of factors affecting the milk production of buffalo.Our findings in this study implied that the g.986 G>T of STAT1 gene might be a candidate molecular marker for milk production traits in buffalo，which provide a foundation for studying the marker-assisted selection programs in Chinese buffaloes.

中图分类号:

邓廷贤，庞春英，朱鹏，段安琴，陆杏蓉，杨炳壮，梁贤威. 水牛STAT1基因多态与产奶性状关联分析[J]. 畜牧兽医学报, 2016, 47(1): 48-54.

DENG Ting-xian，PANG Chun-ying，ZHU Peng，DUAN An-qin，LU Xing-rong，YANG Bing-zhuang，LIANG Xian-wei. Association between Polymorphism of STAT1 Gene and Milk Production Traits in Buffalo (Bubalus bubalis)[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(1): 48-54.

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参考文献

［1］WATSON C J，NEOH K.The Stat family of transcription factors have diverse roles in mammary gland development［J］. Semin Cell Dev Biol，2008，19(4)：401-406.
［2］KHATIB H，HUANG W，MIKHEIL D，et al.Effects of signal transducer and activator of transcription (STAT) genes STAT1 and STAT3 genotypic combinations on fertilization and embryonic survival rates in Holstein cattle［J］. J Dairy Sci， 2009，92(12)：6186-6191.
［3］杨永强，焦仁刚，龚俞，等.务川黑牛STAT3基因启动子区SNPs与生长相关性状的关联分析［J］.畜牧兽医学报，2013，44(8)：1205-1212.
YANG Y Q，JIAO R G，GONG Y，et al.Associations of polymorphisms of promoter region of STAT3 gene with growth traits in Wuchuan black cattle［J］.Acta Veterinaria et Zootechnica Sinica，2013，44(8)：1205-1212.(in Chinese)
［4］BOUTINAUD M，JAMMES H.Growth hormone increases Stat5 and Stat1 expression in lactating goat mammary gland：a specific effect compared to milking frequency［J］.Domest Anim Endocrinol，2004，27(4)：363-378.
［5］MCAVENEY K M，BOOK M L，LING P，et al.Association of 2′，5′-oligoadenylate synthetase with the prolactin (PRL) receptor：alteration in PRL-inducible stat1 (signal transducer and activator of transcription 1) signaling to the IRF-1 (interferon-regulatory factor 1) promoter［J］.Mol Endocrinol，2000，14(2)：295-306.
［6］DENG T，PANG C，ZHU P，et al.Molecular cloning and expression analysis of the STAT1 gene in the water buffalo (Bubalus bubalis)［J］.Trop Anim Health Prod，2015，47(1)：53-59.
［7］宋雪梅，张磊，姜俊芳，等.中国荷斯坦奶牛STAT1全长基因的克隆与序列分析［J］.浙江农业学报，2011，23(5)：880-889.
SONG X M，ZHANG L，JIANG J F，et al.Cloning and analysis of full length sequence of STAT1 gene in Chinese Hostein cow［J］.Acta Agriculture Zhejiangensis，2011，23(5)：880-889.(in Chinese)
［8］WATSON C J.Stat transcription factors in mammary gland development and tumorigenesis［J］.J Mammary Gland Biol Neoplasia，2001，6(1)：115-127.
［9］ASHWELL M S，HEYEN D W，SONSTEGARD T S，et al.Detection of quantitative trait loci affecting milk production，health，and reproductive traits in Holstein cattle［J］.J Dairy Sci，2004，87(2)：468-475.
［10］RON M，FELDMESSER E，GOLIK M，et al.A complete genome scan of the Israeli Holstein population for quantitative trait loci by a daughter design［J］.J Dairy Sci，2004，87(2)：476-490.
［11］COBANOGLU O，ZAITOUN I，CHANG Y M，et al.Effects of the signal transducer and activator of transcription 1 (STAT1) gene on milk production traits in Holstein dairy cattle［J］.J Dairy Sci，2006，89(11)：4433-4437.
［12］褚敏，昝林森.STAT1基因单核苷酸多态性与中国荷斯坦奶牛产奶性状的相关性研究［J］.畜牧兽医学报，2009，40(3)：309-314.
CHU M，ZAN L S.Associations between polymorphisms of STAT1 gene and milk production traits in Chinese Holstein cattle［J］.Acta Veterinaria et Zootechnica Sinica，2009，40(3)：309-314.(in Chinese)
［13］初芹，李东，候诗宇，等.基于DNA池测序法筛选奶牛高信息量SNP标记的可行性［J］.遗传，2014，36(7)：691-696.
CHU Q，LI D，HOU S Y，et al.Direct sequencing of DNA pooling for screening highly informative SNPs in dairy cattle［J］.Hereditas(Beijing)，2014，36(7)：691-696.(in Chinese)
［14］POMBC P.iPLEX^TMAssay：Increased Plexing Efficiency and Flexibility for MassARRAY^® System Through Single Base Primer Extension with Mass-Modified Terminators.Sequenom Application Note.2007.
［15］LIU K J，MUSE S V.PowerMarker：an integrated analysis environment for genetic marker analysis［J］.Bioinformatics，2005，21(9)：2128-2129.
［16］MUI A L.The role of STATs in proliferation，differentiation，and apoptosis［J］.Cell Mol Life Sci，1999，55(12)：1547-1558.
［17］HARICHARAN S，LI Y.STAT signaling in mammary gland differentiation，cell survival and tumorigenesis［J］.Mol Cell Endocrinol，2014，382(1)：560-569.
［18］AU-YEUNG N，MANDHANA R，HORVATH C M.Transcriptional regulation by STAT1 and STAT2 in the interferon JAK-STAT pathway［J］.JAKSTAT，2013，2(3)：e23931.
［19］何峰，孙东晓，俞英，等.荷斯坦奶牛STAT5A基因的SNPs检测及其与产奶性状的关联分析［J］.畜牧兽医学报，2007，38(4)：326-331.
HE F，SUN D X，YU Y，et al.SNPs detection of STAT5A gene and association with milk production traits in Holstein cattle［J］.Acta Veterinaria et Zootechnica Sinica，2007，38(4)：326-331.(in Chinese)
［20］季敏，余长林，余选富，等.槟榔江水牛STAT 5A基因多态性及MspⅠ PCR-RFLP遗传标记的建立［J］.中国牛业科学，2012，38(6)：12-18.
JI M，YU C L，YU X F，et al.Polymorphism and MspⅠ PCR-RFLP genetic marker of STAT5A in Binlangjiang water buffalo［J］.China Cattle Science，2012，38(6)：12-18.(in Chinese)
［21］PAUCIULLO A，COSENZA G，STERI R，et al.A single nucleotide polymorphism in the promoter region of river buffalo stearoyl CoA desaturase gene (SCD) is associated with milk yield［J］.J Dairy Res，2012，79(4)：429-435.
［22］GIL F M，DE CAMARGO G M，PABLOS DE SOUZA F R，et al.Polymorphisms in the ghrelin gene and their associations with milk yield and quality in water buffaloes［J］.J Dairy Sci，2013，96(5)：3326-3331.
［23］贾晋，马妍，孙东晓，等.中国荷斯坦牛DGAT1基因与产奶性状关联分析［J］.畜牧兽医学报，2008，39(12)：1661-1664.
JIA J，MA Y，SUN D X，et al.Association analysis between DGAT1 gene and milk production traits in Chinese Holstein［J］.Acta Veterinaria et Zootechnica Sinica，2008，39(12)：1661-1664.(in Chinese)

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水牛STAT1基因多态与产奶性状关联分析

Association between Polymorphism of STAT1 Gene and Milk Production Traits in Buffalo (Bubalus bubalis)

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