德保矮马X染色体选择信号筛选

doi:10.11843/j.issn.0366-6964.2015.12.006

畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (12): 2161-2168.doi: 10.11843/j.issn.0366-6964.2015.12.006

德保矮马X染色体选择信号筛选

刘雪雪¹，阿地力江•卡德尔¹，董坤哲¹，王月月¹，潘建飞²，浦亚斌¹，何晓红¹，马月辉¹，蒋琳^1*

（1.中国农业科学院北京畜牧兽医研究所，北京 100193； 2.甘肃农业大学动物科学技术学院，兰州 730070）

收稿日期:2015-01-15 出版日期:2015-12-23 发布日期:2015-12-23
通讯作者: 蒋琳，博士，研究员，主要从事动物遗传资源分子评价工作，E-mail：lin.jiang.cas@gmail.com
作者简介:刘雪雪（1991-），女，山西临汾人，硕士生，主要从事动物遗传资源分子评价工作，Tel：010-62815884，E-mail：lxx_caas@163.com
基金资助:
农业科技创新项目（ASTIP-IAS01）；国家自然科学基金项目（31272403）

Detecting Selection Signatures on X Chromosome of Debao Pony

LIU Xue-xue¹，ADLIJIANG Halik¹，DONG Kun-zhe¹，WANG Yue-yue¹，PAN Jian-fei²，PU Ya-bin¹，HE Xiao-hong¹，MA Yue-hui¹，JIANG Lin^1*

(1.Institute of Animal Science，Chinese Academy of Agricultural Sciences，Beijing 100193，China；2.College of Animal Science and Technology，Gansu Agricultural University，Lanzhou 730070，China)

Received:2015-01-15 Online:2015-12-23 Published:2015-12-23

摘要/Abstract

摘要：

旨在对德保矮马（Debao pony）X染色体的选择信号进行筛选。本研究利用Illumina公司开发的马芯片Equine 65K SNP BeadChip，对德保矮马、伊犁马、蒙古马进行X染色体扫描，获得2 339个SNPs位点。通过群体分化系数F_ST法和XP-EHH两种不同的方法，分别以伊犁马和蒙古马为对照群体对德保矮马进行X染色体选择信号检测。结果，筛选到两个受到较强选择区域4.0～39.9和87.1～123.5 Mb，包含64个“离群位点”。通过基因注释筛选到PHEX、BCOR、PNPLA4和GPC3等与生长和骨骼发育相关的基因。研究结果发现，德保矮马的选育过程中X染色体很多与生长相关的基因受到了强烈的选择，其中部分在马中未见报道，可以作为研究矮小性状的重要候选基因。

Abstract:

The study aimed to screen the selection signatures on X chromosome of Debao pony.The Illumina Equine 65K SNP BeadChip was used to perform a X-chromosomal scan on Debao pony，Yili horses and Mongolian horses and obtain 2 339 SNPs with high quality.The result showed that，based on these SNPs，we detected 2 regions(4.0-39.9 and 87.1-123.5 Mb) under strong selection on the X chromosome of Debao pony by 2 different methods，F_ST and XP-EHH.The 2 detected regions contained 64 outliers overlapping genes including BCOR，PHEX，PNPLA4 and GPC3，which are related to the body size and the bone development.The results indicate that genes associated with growth on X chromosome are subject to strong artificial selection pressure during the breeding of the smallest Chinese pony.A few of them are not reported before and could be important candidate genes in future study.

中图分类号:

S821
S821.21

刘雪雪，阿地力江?卡德尔，董坤哲，王月月，潘建飞，浦亚斌，何晓红，马月辉，蒋琳. 德保矮马X染色体选择信号筛选[J]. 畜牧兽医学报, 2015, 46(12): 2161-2168.

LIU Xue-xue，ADLIJIANG Halik，DONG Kun-zhe，WANG Yue-yue，PAN Jian-fei，PU Ya-bin，HE Xiao-hong，MA Yue-hui，JIANG Lin. Detecting Selection Signatures on X Chromosome of Debao Pony[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(12): 2161-2168.

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德保矮马X染色体选择信号筛选

Detecting Selection Signatures on X Chromosome of Debao Pony

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