维拉帕米对肉鸡肺动脉高压发生过程中肺动脉平滑肌细胞c-Fos表达的影响

doi:10.11843/j.issn.0366-6964.2015.08.020

畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (8): 1432-1437.doi: 10.11843/j.issn.0366-6964.2015.08.020

维拉帕米对肉鸡肺动脉高压发生过程中肺动脉平滑肌细胞c-Fos表达的影响

王建琳¹，乔健^2*

（1.青岛农业大学动物科技学院，青岛 266109；2.中国农业大学动物医学院，北京 100193）

收稿日期:2014-10-30 出版日期:2015-08-23 发布日期:2015-08-23
通讯作者: 乔健，教授，博士生导师，E-mail：qiaojian@cau.edu.cn
作者简介:王建琳(1976-)，女，山西沁源人，副教授，博士，主要从事禽病发生机制的研究，E-mail：bjsxxw@126.com
基金资助:
山东省自然科学基金（ZR2013CL022）；国家自然科学基金（30371063）

The Effect of Verapamil on the c-Fos Expression of Pulmonary Artery Smooth Muscular Cells in the Development of Pulmonary Hypertension in Broilers Induced by Low Ambient Temperature

WANG Jian-lin¹，QIAO Jian^2*

(1.College of Animal Science and Veterinary Medicine，Qingdao Agricultural University，Qingdao 266109，China；2.College of Veterinary Medicine，China Agricultural University，Beijing 100193，China)

Received:2014-10-30 Online:2015-08-23 Published:2015-08-23

摘要/Abstract

摘要：

研究低温诱发肉鸡肺动脉高压过程中肺动脉平滑肌细胞c-Fos的表达情况，及钙拮抗剂维拉帕米对c-Fos表达和肺动脉压的影响，从而探讨肉鸡肺动脉高压的发生机制。180只AA肉鸡14日龄随机分为对照组、低温处理组和维拉帕米组，14~49日龄，每组每周随机取6只测定肺动脉压，取肺组织固定做石蜡组织切片进行c-Fos免疫组织化学染色分析。结果显示，低温处理1周后肉鸡肺动脉压明显升高（P<0.05），20~50 μm肺动脉平滑肌细胞c-Fos表达在处理后1周开始明显升高，50~100 μm肺动脉c-Fos表达在处理后2周开始明显升高（P<0.05），而维拉帕米组肉鸡肺动脉压和c-Fos的表达在相应的时间内较低温组明显降低（P<0.05）。结果表明肺动脉平滑肌细胞c-Fos在低温诱发的肉鸡肺动脉压升高的过程中表达增强，阻断钙通道可抑制肉鸡肺动脉平滑肌细胞c-Fos的过高表达和肺动脉压升高。

Abstract:

The c-Fos expression of the pulmonary arterial smooth muscle cells in the development of the pulmonary hypertension in broilers induced by low ambient temperature and the effect of verapamil on c-Fos expression were dynamically studied.One hundred and eighty 14-day-old AA broilers were randomised into three groups，control group (21 ℃±1 ℃)，low temperature group (10 ℃±1 ℃) and verapamil group (10 ℃±1 ℃，add 0.01% verapamil in drinking water).Then their pulmonary arterial pressures were measured；and the lungs of broilers were fixed to study the expression of c-Fos.The results showed that the pulmonary arterial pressure in low temperature group significantly higher than those in control group and verapamil group after exposure to low temperature one week (P<0.05)，the expression of c-Fos in 20-50 μm and 50-100 μm pulmonary arteries in low temperature group were significantly higher than those in control group and verapamil group after exposure to low temperature one and two weeks respectively (P<0.05).The results suggested that the expression of c-Fos in pulmonary arteries were significantly increased and verapamil can prevent the high expression of c-Fos and the increase of pulmonary arterial pressure.

中图分类号:

S852.33

王建琳，乔健. 维拉帕米对肉鸡肺动脉高压发生过程中肺动脉平滑肌细胞c-Fos表达的影响[J]. 畜牧兽医学报, 2015, 46(8): 1432-1437.

WANG Jian-lin，QIAO Jian. The Effect of Verapamil on the c-Fos Expression of Pulmonary Artery Smooth Muscular Cells in the Development of Pulmonary Hypertension in Broilers Induced by Low Ambient Temperature[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(8): 1432-1437.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.xmsyxb.com/CN/10.11843/j.issn.0366-6964.2015.08.020

https://www.xmsyxb.com/CN/Y2015/V46/I8/1432

参考文献

［1］JULIAN R J.Ascites in poultry［J］.Avian Pathol，1993，22(3)：419-454.
［2］WIDEMAN R F，RHOADS D D，ERF G F，et al.Pulmonary arterial hypertension (ascites syndrome) in broilers：a review［J］.Poult Sci，2013，92(1)：64-83.
［3］王建琳，乔健，赵立红，等.低温诱发肉鸡腹水综合征过程中肺动脉平滑肌细胞增殖的变化［J］.畜牧兽医学报，2007，38(3)：276-281.
WANG J L，QIAO J，ZHAO L H，et al.Proliferation of pulmonary artery smooth muscle cells in the development of ascites syndrome induced by low ambient temperature in broilers［J］.Acta Veterinaria et Zootechnica Sinica，2007，38(3)：276-281.(in Chinese)
［4］王建琳，乔健，赵立红，等.环境低温对肉鸡肺小动脉壁c-jun mRNA 表达的影响［J］.中国农业大学学报，2006，11(6)：83-86.
WANG J L，QIAO J，ZHAO L H，et al.Effect of low ambient temperature on expression of c-jun mRNA oncogene in the wall of pulmonary arteries in broilers［J］.Journal of China Agricultural University，2006，11(6)：83-86.(in Chinese)
［5］王建琳，尹燕博.低温诱发肉鸡肺血管重塑过程中肺动脉平滑肌细胞c-Myc蛋白表达的变化［J］.畜牧兽医学报，2011，42(1)：99-103.
WANG J L，YIN Y B.Expression of c-Myc in pulmonary vascular smooth muscel cells in the development of pulmonary vascular remodeling in broilers induced by low ambient temperature［J］.Acta Veterinaria et Zootechnica Sinica，2011，42(1)：99-103.(in Chinese)
［6］王建琳，乔健，赵立红，等.维拉帕米对环境低温诱发肉鸡肺动脉平滑肌细胞增殖的作用［J］.中国兽医杂志，2007，43(12)：29-30.
WANG J L，QIAO J，ZHAO L H，et al.The effect of verapamil on the proliferation of the smooth muscle cells in pulmonary arteries of chicken raised in low temperature［J］.Chinese Journal of Veterinary Medicine，2007，43(12)：29-30.(in Chinese)
［7］张建军，孙茂红，王黎霞，等.维拉帕米对低温诱发的肉鸡肺血管重塑的影响［J］.中国农学通报，2014，30(23)：8-12.
ZHANG J J，SUN M H，WANG L X，et al.Effect of verapamil on pulmonary vascular remodeling in broiler chickens induced by low ambient temperature［J］.Chinese Agricultural Science Bulletin，2014，30(23)：8-12.(in Chinese)
［8］BIASIN V，CHWALEK K，WILHELM J，et al.Endothelin-1 driven proliferation of pulmonary arterial smooth muscle cells is c-fos dependent［J］.Int J Biochem Cell Biol，2014，54：137-148.
［9］CHEN X，LIU H，PAN Z，et al.The inhibitory effects of m-nisoldipine on the 5-hydroxytryptamine-induced proliferation of pulmonary artery smooth muscle cells via Ca²⁺ antagonism and antioxidant mechanisms［J］.Eur J Pharmacol，2012，686(1-3)：32-40.
［10］ZHANG J，FENG X，ZHAO L，et al.Expression of hypoxia-inducible factor 1α mRNA in hearts and lungs of broiler chickens with ascites syndrome induced by excess salt in drinking water［J］.Poult Sci，2013，92(8)：2044-2052.
［11］LI J C，PAN J Q，HUANG G Q，et al.Expression of PDGF-beta receptor in broilers with pulmonary hypertension induced by cold temperature and its association with pulmonary vascular remodeling［J］.Res Vet Sci，2010，88(1)：116-121.
［12］YANG Y，QIAO J，WNAG H，et al.Calcium antagonist verapamil prevented pulmonary arterial hypertension in broilers with ascites by arresting pulmonary vascular remodeling［J］.Eur J Pharmacol，2007，561(1-3)：137-143.
［13］王建琳，乔健，赵立红，等.低温诱发肉鸡腹水综合征发生过程中肺血管重塑与肺动脉高压的关系［J］.中国兽医学报，2007，27(6)：874-878.
WANG J L，QIAO J，ZHAO L H，et al.Relationship between pulmonary vascular remodeling and pulmonary arterial pressure in ascites syndrom induced by low ambient temperature in broilers［J］.Chinese Journal of Verterinary Science，2007，27(6)：874-878.(in Chinese)
［14］STENMARK K R，FAQAN K A，FRID M G.Hypoxia-induced pulmonary vascular remodeling：cellular and molecular mechanisms［J］.Circ Res，2006，99(7)：675-691.
［15］董世山，利凯，王迎春，等.钙信号转导与缺氧诱发肉鸡肺动脉平滑肌细胞癌基因c-fos、c-myc表达的关系［J］.畜牧兽医学报，2006，37(8)：824-828.
DONG S S，LI K，WANG Y C，et al.The relationship between calcium signal transduction and expression of c-fos，c-myc in broiler’s pulmonary arterial smooth muscle cell induced by hypoxia［J］.Acta Veterinaria et Zootechnica Sinica，2006，37(8)：824-828.(in Chinese)
［16］YAMAMURA A，YAMAMURA H，YUAN J X.Enhanced Ca2+-sensing receptor function in pulmonary hypertension［J］.Yakuqaku Zasshi，2013，133(12)：1351-1359.

[1]	张海洋, 朱秋祥, 高志诚, 贾坤, 王京煜, 冀锦朝, 梁雾滢, 莫睿文, 李守军. 猫急性应激模型的建立及诊断标志物的筛选[J]. 畜牧兽医学报, 2023, 54(3): 1249-1260.
[2]	谭天宇, 才冬杰, 苟丽萍, 任志华, 左之才. 基于RAGE-TLR4串扰的高糖影响牛肺泡巨噬细胞促炎细胞因子释放的分子机制[J]. 畜牧兽医学报, 2021, 52(10): 2944-2952.
[3]	杨玲, 杨汶菱, 张绘艳, 卞建春, 刘宗平, 袁燕, 邹辉, 顾建红, 刘学忠. 棕榈酸通过上调ACC/FAS/DGAT2通路表达诱导BRL 3A细胞脂肪变性[J]. 畜牧兽医学报, 2019, 50(12): 2537-2544.
[4]	李军, 高晶萍, 张雅琪, 罗强, 梁亭, 李珮瑶, 王少华, 张瑞华, 徐明举, 徐彤. TRPM2离子通道在H9N2流感病毒感染小鼠肺微血管内皮细胞线粒体损伤中的作用[J]. 畜牧兽医学报, 2019, 50(10): 2053-2060.
[5]	廖政, 李基棕, 肖芳, 钟纯燕, 杨蕾蕾, 毛立, 李文良, 孙敏, 主性, 嵇辛勤, 张纹纹, 刘茂军. 山羊副流感病毒3型通过Caspase途径诱导气管上皮细胞凋亡[J]. 畜牧兽医学报, 2019, 50(10): 2061-2069.
[6]	陈洪博, 段滇宁, 鲍恩东. 热应激致Hsc70出入核与细胞凋亡的关系[J]. 畜牧兽医学报, 2017, 48(12): 2414-2420.
[7]	韩亚楠，杨晨玉，王子旭，陈耀星，孙佳毅，秦卓明，曹静，董玉兰. 5-HT对断奶仔鼠应激腹泻时肠道抗氧化功能的影响[J]. 畜牧兽医学报, 2014, 45(12): 2067-2073.
[8]	韩俊源，郭华，张亚群，段滇宁，李晓琳，陈萌萌，张书霞. PCV2通过激活MyD88调控体外培养PAMs分泌IL-1β、IL-6和IL-10[J]. 畜牧兽医学报, 2014, 45(4): 647-653.
[9]	田文霞，李家奎，王瑞，覃平，宁官保，乔建钢，李宏全，毕丁仁，潘思轶，郭定宗. 四甲基秋兰姆二硫化物对肉鸡胫骨生长板超微结构的影响[J]. 畜牧兽医学报, 2013, 44(6): 965-971.
[10]	吕春子，代蕾，郭华，张书霞. 猪圆环病毒2型对体外培养仔猪淋巴细胞NF-κB信号的动态影响[J]. 畜牧兽医学报, 2013, 44(5): 760-766.

维拉帕米对肉鸡肺动脉高压发生过程中肺动脉平滑肌细胞c-Fos表达的影响

The Effect of Verapamil on the c-Fos Expression of Pulmonary Artery Smooth Muscular Cells in the Development of Pulmonary Hypertension in Broilers Induced by Low Ambient Temperature

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 10

编辑推荐

Metrics

本文评价