畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (8): 1610-1617.doi: doi: 10.11843/j.issn.0366-6964.2016.08.011

• 预防兽医 • 上一篇    下一篇

中国部分地区鸭、鹅携带四种病毒状况的调查与分析

刘洋,王传彬,霍斯琪,翟新验,辛盛鹏,刘玉良,韩雪,张倩,杨卫铮,顾小雪*   

  1. (中国动物疫病预防控制中心,北京 102618)
  • 收稿日期:2016-02-29 出版日期:2016-08-23 发布日期:2016-08-23
  • 通讯作者: 顾小雪,E-mail:gxxjackie@163.com
  • 作者简介:刘洋(1985-),女,山西大同人,兽医师,博士,主要从事水禽疫病及禽类细菌病的防控工作,E-mail:lysonna@163.com
  • 基金资助:

    农业技术试验示范项目(2130106);科技基础性工作专项(2013FY113300)

Epidemiologic Investigation of Four Viruses Carried by Ducks and Geese in China

LIU Yang,WANG Chuan-bin,HUO Si-qi,ZHAI Xin-yan,XIN Sheng-peng,LIU Yu-liang,HAN Xue,ZHANG Qian,YANG Wei-zheng,GU Xiao-xue*   

  1. (China Animal Disease Control Centre,Beijing 100126,China)
  • Received:2016-02-29 Online:2016-08-23 Published:2016-08-23

摘要:

为了解中国部分地区鸭、鹅的病毒携带情况,作者于2014年11月,从安徽、浙江、福建、广东、广西5省的多个表观健康鸭、鹅群中采集样品1 931份,采用荧光定量PCR对鸭瘟病毒(DPV)、鸭甲型肝炎病毒1型(DHAV-1)、小鹅瘟病毒(GPV)、鸭坦布苏病毒(DTMUV)进行检测。结果显示,DTMUV阳性率为1.2%,DPV阳性率为0.3%;其余两种病毒均未检出。结果表明,临床表观健康的鸭、鹅可携带鸭坦布苏病毒和鸭瘟病毒,而且,序列分析结果显示,所携带的毒株与临床分离的相应致病性毒株相似性较高,提示在水禽疫病传播过程中,表观健康但带毒的动物可能成为疫病传播的风险点。

Abstract:

 To investigate the prevalence of duck plague virus (DPV),duck hepatitis virus type 1 (DHAV-1),goose parvovirus (GPV) and duck Tembusu virus (DTMUV) carried by duck and goose flocks in China,1 931 samples were collected from clinically healthy ducks and geese in Anhui,Zhejiang,Fujian,Guangdong and Guangxi provinces in November,2014.The four viruses above were detected by real-time PCR.DTMUV and DPV were detected in these specimens with a positive rate of 1.2% and 0.3%,respectively,while no DHAV-1 or GPV was detected.Our results demonstrated the presence of DTMUV and DPV in clinically healthy duck and goose flocks,furthermore,the two viruses carried by duck and goose shared high similarities at the nucleic acid level with the corresponding clinical pathogenic strains.These findings highlight the necessity of active surveillance for healthy waterfowls,in order to prevent transmission of viruses.

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