高原地区小尾寒羊附睾细胞外基质相关蛋白分布特征研究

doi:doi: 10.11843/j.issn.0366-6964.2016.08.006

摘要/Abstract

摘要：

旨在研究高原地区小尾寒羊附睾细胞外基质相关蛋白的分布特征。应用Masson’s、Gomori’s、PAS和 AB-PAS（pH=2.5）组织化学染色方法观察5只健康成年高原地区小尾寒羊附睾的组织结构特点，进而用免疫组织化学SP法观察层粘连蛋白（LN）、Ⅳ型胶原蛋白（Col Ⅳ）和硫酸乙酰肝素蛋白多糖（HSPG）的分布特征。高原地区小尾寒羊附睾各部分管腔为柱状纤毛上皮，附睾尾间质胶原纤维和网状纤维较附睾头及附睾体明显增多。PAS反应显示附睾头和附睾尾阳性强于附睾体，AB-PAS反应显示附睾头、体、尾阳性差异较小。免疫组织化学显示，Col Ⅳ在附睾头和附睾体组织中表达最强，HSPG次之，LN表达较弱，HSPG和LN的阳性表达差异不显著（P＞0.05）；而HSPG在附睾尾组织中表达最强，LN次之，Col Ⅳ表达较弱，HSPG和LN的阳性表达差异不显著（P＞0.05）。高原地区小尾寒羊附睾尾间质胶原纤维和网状纤维的分布较附睾头和附睾体多，附睾各部分中PAS的反应强弱与附睾上皮分泌功能的变化密切相关，附睾头和附睾尾输精管的分泌功能增强。附睾头上皮基细胞Col Ⅳ、HSPG和LN分泌增加，且Col Ⅳ、HSPG和LN与血管的调节作用密切相关。

Abstract:

To explore the distribution of extracellular matrix proteins in epididymis of Small-tail Han sheep in plateau.The histochemistry of Masson’s，Gomori’s，PAS and AB-PAS（pH=2.5）staining were used to study the microstructure of epididymis in 5 pairs of Small-tail Han sheep in plateau，and the distribution of the laminin (LN)，type Ⅳ collagen (Col Ⅳ) and heparan sulfate glycoprotein (HSPG) were identified by SP immunohistochemical method.The observations made with the light microscope showed that the epithelium of epididymal ducts were columnar ciliated and the distribution of the collagen and reticular fibers in the cauda were more abundant than the caput and the corpus，although the PAS positive reaction in the caput and the cauda were stronger than the corpus，but the AB-PAS positive reaction without significant differences in the caput，the corpus and the cauda.Immunohistochemical analysis appeared that Col Ⅳ was strongly presented in the caput and the corpus，the relative expression of HSPG was lower than Col Ⅳ，and LN was expressed weakly，the distribution of LN and HSPG without statistical differences in epididymis (P＞0.05)，but in the cauda，it was showed that HSPG was strongly presented，the Col Ⅳ was lower than LN and HSPG，and the distribution of LN and HSPG without statistical differences in epididymis (P＞0.05).Taken together，the collagen and reticular fibers of the epididymis cauda were more abundant than the caput and corpus in Small-tail Han sheep from plateau，and the positive of the PAS reaction was companied with the changes of the secretion function of epithelium.The secretion of Col Ⅳ，HSPG and LN in the basal cells of caput epithelium were increased than the other parts，and the Col Ⅳ，HSPG and LN might work together closely for the regulation of the vessel.

中图分类号:

S852.16+2

李聪，袁莉刚，张勇. 高原地区小尾寒羊附睾细胞外基质相关蛋白分布特征研究[J]. 畜牧兽医学报, 2016, 47(8): 1565-1573.

LI Cong，YUAN Li-gang，ZHANG Yong. The Distribution of Extracellular Matrix Proteins in Small-tail Han Sheep Epididymis in Plateau[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(8): 1565-1573.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.xmsyxb.com/CN/doi: 10.11843/j.issn.0366-6964.2016.08.006

https://www.xmsyxb.com/CN/Y2016/V47/I8/1565

参考文献

［1］张英杰，刘月琴，储明星.小尾寒羊高繁殖力和常年发情内分泌机理的研究［J］.畜牧兽医学报，2001，32(6)：510-516.
ZHANG Y J，LIU Y Q，CHU M X.Study on endocrine basis of prolificacy and non-seasonal estrus in small tail han sheep［J］.Acta Veterinaria et Zootechnica Sinica，2001，32(6)：510-516.(in Chinese)
［2］成令忠.组织学［M］.第二版.北京：人民卫生出版社，1994.
CHENG L Z.Histology［M］.2nd ed.Beijing：People’s Medical Press，1994.(in Chinese)
［3］SWINEHART I T，BADYLAK S F.Extracellular matrix bioscaffolds in tissue remodeling and morphogenesis［J］.Dev Dyn，2016，245(3)：351-360.
［4］宋阳，许增禄.睾丸细胞外间质作用的研究［J］.解剖学报，1997，28(2)：221-223.
SONG Y，XU Z L.Study on role of the testis extracellular matrix［J］.Acta Anatomica Sinica，1997，28(2)：221-223.(in Chinese)
［5］HUDSON B G，REEDERS S T，TRYGGVASON K.Type Ⅳ collagen：structure，gene organization，and role in human diseases.Molecular basis of Goodpasture and Alport syndromes and diffuse leiomyomatosis［J］.J Biol Chem，1993，268(35)：26033-26036.
［6］FARACH-CARSON M C，WARREN C R，HARRINGTON D A，et al.Border patrol：insights into the unique role of perlecan/heparan sulfate proteoglycan 2 at cell and tissue borders［J］.Matrix Biol，2014，34：64-79.
［7］KRUEGEL J，MIOSGE N.Basement membrane components are key players in specialized extracellular matrices［J］.Cell Mol Life Sci，2010，67(17)：2879-2895.
［8］崔丽，许增禄，刘霄，等.小鼠附睾Ⅳ型胶原和层粘连蛋白的分布［J］.解剖学报，1999，30(3)：273-275.
CUI L，XU Z L，LIU X，et al.Distribution of type Ⅳ collagen and laminin in the mice epididymis［J］.Acta Anatomica Sinica，1999，30(3)：273-275.(in Chinese)
［9］李聪，袁莉刚，曲亚玲，等.高原型藏绵羊附睾细胞外基质的组织化学研究［J］.畜牧兽医学报，2016，47(4)：820-827.
LI C，YUAN L G，QU Y L，et al.The histochemical study of extracellular matrix in plateau tibetan sheep epididymis［J］.Acta Veterinaria et Zootechnica Sinica，2016，47(4)：820-827.(in Chinese)
［10］陶凯忠，陈尔瑜，丁光宏.胶原纤维的结构和生物力学［J］.解剖科学进展，1998，4(4)：289-293.
TAO K Z，CHEN E Y，DING G H.The structure and biomechanics of collagen fibers［J］.Progress of Anatomical Sciences，1998，4(4)：289-293.(in Chinese)
［11］崔丽，刘霄，许增禄，等.小鼠附睾细胞外间质发育的研究［J］.中国医学科学院学报，1996，18(4)：318.
CUI L，LIU X，XU Z L，et al.Study of extracellular matrix in the mice epididymis (briefing)［J］. Acta Academiae Medicinae Sinicae， 1996，18(4)：318.(in Chinese)
［12］KEENE D R，SAKAI L Y，BÄCHINGER H P，et al.Type III collagen can be present on banded collagen fibrils regardless of fibril diameter［J］.J Cell Biol，1987，105(5)：2393-2402.
［13］蔡新华，郭志坤，毛会丽，等.大鼠心肌组织中网状纤维组织学构筑及增龄变化［J］.解剖学报，2009，40(1)：127-129.
CAI X H，GUO Z K，MAO H L，et al.Histological architecture of reticular fibers and age-related changes of myocardial tissue in rats［J］.Acta Anatomica Sinica， 2009，40(1)：127-129.(in Chinese)
［14］上海第一医学院.组织学［M］.北京：人民卫生出版社，1981.
The First Medical College in Shanghai.Histology［M］.Beijing：People’s Medical Press，1981.(in Chinese)
［15］FAKOYA F A.Reticulin fibres in the tunica albuginea and peritubular tissue of seminiferous tubules of adult male Wistar rats［J］.Acta Histochem，2002，104(3)：279-283.
［16］WASSARMAN P M，LITSCHER E S.A bespoke coat for eggs：getting ready for fertilization［J］.Curr Top Dev Biol，2016，117：539-552.
［17］MOORE B C，HAMLIN H J，BOTTERI N L，et al.Posthatching development of Alligator mississippiensis ovary and testis［J］.J Morphol，2010，271(5)：580-595.
［18］GLEGG R E，EIDINGER D，LEBLOND C P.Some carbohydrate components of reticular fibers［J］.Science，1953，118(3073)：614-616.
［19］薛同一，谢蔚英，朱继业.大鼠、牛、猪和羊附睾上皮内富有PAS阳性颗粒细胞的组织学观察［J］.解剖学报，1984，15(3)：318-320.
XUE T Y，XIE W Y，ZHU J Y.Histological observation on PAS-positive granular cells in epididymal epithelium from rat，bull，swine and ram［J］. Acta Anatomica Sinica，1984，15(3)：318-320.(in Chinese)
［20］汤美蓉，齐易详.大白鼠附睾的结构和功能Ⅰ.组织学和PAS反应［J］.皖南医学院学报，1987，6(2)：108-111.
TANG M R，QI Y X.Structure and function of the rat epididymis.I.Histology and PAS reaction［J］.Mcta Academiae Medicinae Wannan，1987，6(2)：108-111.(in Chinese)
［21］HARVEY S J，PERRY J，ZHENG K，et al.Sequential expression of type Ⅳ collagen networks：testis as a model and relevance to spermatogenesis［J］.Am J Pathol，2006，168(5)：1587-1597.
［22］李戈，杨程显，李煜生.胶原蛋白Ⅳ的生物学特性研究进展［J］.生命科学，2014，26(9)：931-935.
LI G，YANG C X，LI Y S.Research progress of molecular and biological characteristics of type Ⅳ collagen［J］.Chinese Bulletin of Life Sciences， 2014，26(9)：931-935.(in Chinese)
［23］OGUZKURT P，KAYASELUK F，TUNCER I，et al.Evaluation of extracelluar matrix protein composition in sacs associated with undescended testis，hydrocele，inguinal hernia，and peritoneum［J］.Urology，2007，70(2)：346-350.
［24］许志强，刘平.细胞外基质的结构与功能［J］.肝脏，1999，4(2)：92-93.
XU Z Q，LIU P.The structure and function of extracellular matrix［J］.Chinese Hepatology，1999，4(2)：92-93.(in Chinese)
［25］袁莉刚，曲亚玲，朱俊峰，等.牦牛犊牛睾丸结构特征及细胞外基质相关蛋白的分布［J］.解剖学报，2015，46(1)：106-112.
YUAN L G，QU Y L，ZHU J F，et al.Structure and distribution of extracellular matrix proteins in yak calves testis［J］.Acta Anatomica Sinica， 2015，46(1)：106-112.(in Chinese)
［26］姜笃银，付小兵，盛志勇.硫酸乙酰肝素糖蛋白的结构-功能多样性与相关修饰酶群作用［J］.中国病理生理杂志，2005，21(5)：1020-1025.
JIANG D Y，FU X B，SHENG Z Y.The diversity of structure and function of heparin sulfate proteoglycans via modification of some relative enzymes［J］.Chinese Journal of Pathophysiology，2005，21(5)：1020-1025.(in Chinese)
［27］HAYASHI K，HAYASHI M，JALKANEN M，et al.Immunocytochemistry of cell surface heparan sulfate proteoglycan in mouse tissues.A light and electron microscopic study［J］.J Histochem Cytochem，1987，35(10)：1079-1088.
［28］李媛，崔慧斐.硫酸乙酰肝素蛋白聚糖与血管形成［J］.食品与药品，2005，7(8)：19-23.
LI Y，CUI H F.Heparan sulfate proteoglycan and angiogenesis［J］.Food and Drug， 2005，7(8)：19-23.(in Chinese)
［29］PRESTA M，LEALI D，STABILE H，et al.Heparin derivatives as angiogenesis inhibitors［J］.Curr Pharm Des，2003，9(7)：553-566.
［30］谭兽鲁，周柔丽.医学细胞生物学［M］.北京：北京医科大学、中国协和医科大学联合出版社，1992.
TAN S L，ZHOU R L.Medical cell biology［M］.Beijing：Beijing Medical University and Chinese Peking Union Medical College Unit Press，1992.(in Chinese)
［31］陈光，贺占国，王永海，等.层粘蛋白在颌面部毛细血管瘤组织中的表达［J］.实用口腔医学杂志，1998，14(1)：9-10.
CHEN G，HE Z G，WANG Y H，et al.The expression of laminin in maxillofacial capillary hemangioma［J］.Journal of Practical Stomatology，1998，14(1)：9-10.(in Chinese)
［32］BEHRENS T，VILLONE D，KOCH M，et al.The epidermal basement membrane is a composite of separate laminin-or collagen Ⅳ-containing networks connected by aggregated perlecan，but not by nidogens［J］.J Biol Chem，2012，287(22)：18700-18709.
［33］RAGHAVAN S，GILMONT R R，BITAR K N.Neuroglial differentiation of adult enteric neuronal progenitor cells as a function of extracellular matrix composition［J］.Biomaterials，2013，34(28)：6649-6658.