表没食子儿茶素没食子酸酯对育肥猪骨骼肌纤维类型的影响

doi:doi: 10.11843/j.issn.0366-6964.2016.08.008

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (8): 1581-1591.doi: doi: 10.11843/j.issn.0366-6964.2016.08.008

表没食子儿茶素没食子酸酯对育肥猪骨骼肌纤维类型的影响

王丽娜，王珍，彭建龙，王建兵，杨柯林，束刚，王松波，朱晓彤，高萍，江青艳^*

（华南农业大学动物科学学院，国家生猪种业工程技术研究中心，广州 510642）

收稿日期:2015-09-06 出版日期:2016-08-23 发布日期:2016-08-23
通讯作者: 江青艳，教授，博士生导师，E-mail：qyjiang@scau.edu.cn
作者简介:王丽娜（1982），女，山西太谷人，讲师，硕士生导师，主要从事动物营养生理研究，E-mail：wanglina@scau.edu.cn
基金资助:
国家重点基础研究发展计划（2012CB124701）；国家自然科学基金青年基金（31101780）；博士点基金新教师类（20114404120001）

Effects of EGCG on Muscle Fiber Types of Finishing Pigs

WANG Li-na，WANG Zhen，PENG Jian-long，WANG Jian-bing，YANG Ke-lin，SHU Gang，WANG Song-bo，ZHU Xiao-tong，GAO Ping，JIANG Qing-yan^*

(National Engineering Research Center For Breeding Swine Industry，College of Animal Science，South China Agricultural University，Guangzhou 510642，China)

Received:2015-09-06 Online:2016-08-23 Published:2016-08-23

摘要/Abstract

摘要：

本试验主要研究在饲粮中添加表没食子儿茶素没食子酸酯（Epigallocatechin gallate，EGCG）对育肥猪肌纤维类型的影响。试验选用156日龄“杜×长×大”三元杂交育肥猪180头，平均体重为(85.02±1.15) kg，按组间体重、性别比例一致的原则，将育肥猪随机分成3组，每组6个重复，每个重复10头猪（5公5母）。对照组饲喂基础饲粮，试验组饲喂在基础饲粮上分别添加0.025%、0.05% EGCG的试验饲粮。研究结果表明：1）育肥猪饲粮中添加0.025%的EGCG，能显著下调背最长肌乳酸脱氢酶（Lactate dehydrogenase，LDH）的活性（P<0.05），减少MyHCⅠ、PGC-1α和mtTFA蛋白水平和MyHCⅠ、Tnnt 1、Cytc和COXⅣmRNA的表达（P<0.05）；显著下调腰大肌LDH和琥珀酸脱氢酶（Succinate dehydrogenase，SDH）的活性（P<0.05），显著上调Ⅰ型肌纤维比例（P<0.05），显著下调MyHC ⅠmRNA的表达（P<0.05），而MyHCⅡa、MyHCⅡx、MyHCⅡb 的mRNA显著上调（P<0.05）。2）育肥猪饲粮中添加0.050%的EGCG，能显著下调背最长肌LDH和SDH的活性（P<0.05），降低活性氧（Reactive oxygen species，ROS）水平（P<0.05），抑制AMPK的磷酸化、下调MyHC Ⅰ、PGC-1α、mtTFA和NRF-1的蛋白和mRNA表达（P<0.05）；显著下调腰大肌LDH和SDH的活性（P<0.05），降低ROS水平（P<0.05），下调MyHC Ⅰ蛋白和mRNA的表达、抑制AMPK的磷酸化和NRF-1的蛋白表达（P<0.05），显著上调Ⅰ型肌纤维比例（P<0.05）。综上，育肥猪饲粮中添加EGCG后，可能通过EGCG清除ROS后引起AMPK活性减弱，降低PGC-1α表达，最终导致线粒体生物合成下降和慢肌纤维形成减少。

Abstract:

The aim of this study was to investigate the effects of Epigallocatechin Gallate (EGCG) on muscle fiber types of finishing pigs.One-hundred eighty crossbred finishing pigs (Duroc × Yorkshire × Landrace，156-day-old) with an average body weight of (85.02 ± 1.15) kg were selected for this study.According to the consistent principle of weight and sex ratio between groups，pigs were randomly divided into 3 groups，and each group with 6 repeats (10 pigs/repeat(5 male，5 female)).Control group was fed with basal diet，treatment groups were fed with basal diet supplemented with 0.025% and 0.05% EGCG，respectively.The results showed that：1) 0.025% EGCG could significantly decrease the activity of LDH，the protein level of MyHCⅠ，PGC-1α，mtTFA and mRNA expression of MyHCⅠ， Tnnt 1，Cytc and COXⅣ in pigs longissimus dorsi (P<0.05)；in psoas major muscle，the activity of LDH and SDH and mRNA expression of MyHC Ⅰwas reduced，the proportion of typeⅠmuscle fiber and mRNA expression of MyHCⅡa，MyHCⅡx，MyHCⅡb were upregulated (P<0.05).2) In longissimus dorsi，0.05% EGCG treatment could significantly decrease the activity of LDH and SDH (P<0.05)，the level of ROS (P<0.05)，and activity of AMPK，the level of protein and mRNA expression of MyHCⅠ，PGC-1α，mtTFA and NRF-1 (P<0.05).While，in psoas major muscle，0.050% EGCG treatment reduced the activity of LDH and SDH (P<0.05)，the level of ROS (P<0.05)，and the protein level and mRNA expression of MyHCⅠ，inhibited the phosphorylation of AMPK and protein expression of NRF-1 (P<0.05)，but the proportion of typeⅠmuscle fiber was upregulated.In conclusion，the effect of EGCG on longissimus dorsi and psoas major of pigs might be completed by reducing ROS level，thus decreasing the AMPK activity and the expression of PGC-1α，eventually reducing the formation of slow-twitch muscle fibers and mitochondria biosynthesis.

中图分类号:

S828
S821.2

王丽娜，王珍，彭建龙，王建兵，杨柯林，束刚，王松波，朱晓彤，高萍，江青艳. 表没食子儿茶素没食子酸酯对育肥猪骨骼肌纤维类型的影响[J]. 畜牧兽医学报, 2016, 47(8): 1581-1591.

WANG Li-na，WANG Zhen，PENG Jian-long，WANG Jian-bing，YANG Ke-lin，SHU Gang，WANG Song-bo，ZHU Xiao-tong，GAO Ping，JIANG Qing-yan. Effects of EGCG on Muscle Fiber Types of Finishing Pigs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(8): 1581-1591.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.xmsyxb.com/CN/doi: 10.11843/j.issn.0366-6964.2016.08.008

https://www.xmsyxb.com/CN/Y2016/V47/I8/1581

参考文献

［1］杨飞云，陈代文，黄金秀，等.猪背最长肌肌纤维类型的发育性变化及品种与营养影响特点［J］.畜牧兽医学报，2008，39(12)：1701-1708.
YANG F Y，CHEN D W，HUANG J X，et al.Developmental changes of myofiber types in longissimus dorsi muscle of Rongchang and DLY pigs under different nutrient condition［J］.Acta Veterinaria et Zootechnica Sinica，2008，39(12)：1701-1708.(in Chinese)
［2］CANEPARI M，PELLEGRINO M A，D’ANTONA G，et al.Skeletal muscle fiber diversity and the underlying mechanisms［J］.Acta Physiol (Oxf)，2010，199(4)：465-476.
［3］FLUECK M.Tuning of mitochondrial pathways by muscle work：from triggers to sensors and expression signatures［J］. Appl Physiol Nutr Metab，2009，34(3)：447-453.
［4］IRRCHER I，LJUBICIC V，HOOD D A.Interactions between ROS and AMP kinase activity in the regulation of PGC-1α transcription in skeletal muscle cells［J］.Am J Physiol Cell Physiol，2009，296(1)：C116-C123.
［5］KANG C，O’MOORE K M，DICKMAN J R，et al.Exercise activation of muscle peroxisome proliferator-activated receptor-γ coactivator-1α signaling is redox sensitive［J］.Free Radic Biol Med，2009，47(10)：1394-1400.
［6］LIN J，WU H，TARR P T，et al.Transcriptional co-activator PGC-1 alpha drives the formation of slow-twitch muscle fibres［J］.Nature，2002，418(6899)：797-801.
［7］WU Z，PUIGSERVER P，ANDERSSON U，et al.Mechanisms controlling mitochondrial biogenesis and respiration through the thermogenic coactivator PGC-1［J］.Cell，1999，98：115-124.
［8］BALABAN R S，NEMOTO S，FINKEL T.Mitochondria，oxidants，and aging［J］.Cell，2005，120(4)：483-495.
［9］DRÖGE W.Free radicals in the physiological control of cell function［J］.Physiol Rev，2002，82(1)：47-95.
［10］ALLEN R G，TRESINI M.Oxidative stress and gene regulation［J］.Free Radic Biol Med，2000，28(3)：463-499.
［11］HAWLEY J A，ZIERATH J R.Integration of metabolic and mitogenic signal transduction in skeletal muscle［J］.Exerc Sport Sci Rev，2004，32(1)：4-8.
［12］JI L L.Antioxidant signaling in skeletal muscle：a brief review［J］.Exp Gerontol，2007，42(7)：582-593.
［13］OYEDOTUN K S，LEMIRE B D.The quaternary structure of the Saccharomyces cerevisiae succinate dehydrogenase.Homology modeling，cofactor docking，and molecular dynamics simulation studies［J］.J Biol Chem，2004，279(10)：9424-9431.
［14］CHEN W C，HSIEH S R，CHIU C H，et al.Molecular identification for epigallocatechin-3-gallate-mediated antioxidant intervention on the H2O2-induced oxidative stress in H9c2 rat cardiomyoblasts［J］.J Biomed Sci，2014，21：56.
［15］吴秀琴，杨威，尹玉娇，等.茶多酚对一次性力竭运动大鼠氧化应激和炎症反应的影响［J］.中国体育科技，2016，52(1)：92-95.
WU X Q，YANG W，YIN Y J，et al.The effects of tea polyphenols supplement on oxidative stress and inflammatory response in rats after exhaustive exercise［J］.China Sport Science and Technology，2016，52(1)：92-95.(in Chinese)
［16］李爽，艾英伟，阿拉木斯，等.黄芪总苷对运动性疲劳大鼠骨骼肌抗氧化能力、ATP酶活性及乳酸含量的影响［J］.吉林体育学院学报，2010，26(3)：68-69.
LI S，AI Y W，ALA M，et al.Effects of astragalosides on antioxidant capacity，ATPase activity and content of lactic acid in skeletal muscles of rats with exercise induced fatigue［J］. Journal of Jilin Institute of Physical Education，2010，26(3)：68-69.(in Chinese)
［17］ZHONG R Z，ZHOU D W，TAN C Y，et al.Effect of tea catechins on regulation of antioxidant enzyme expression in H2O2-induced skeletal muscle cells of goat in vitro［J］. J Agric Food Chem，2011，59(20)：11338-11343.
［18］CHENG T H，SHIH N L，CHEN C H，et al.Role of mitogen-activated protein kinase pathway in reactive oxygen species-mediated endothelin-1-induced beta-myosin heavy chain gene expression and cardiomyocyte hypertrophy［J］. J Biomed Sci，2005，12(1)：123-133.
［19］HARDIE D G，SAKAMOTO K.AMPK：a key sensor of fuel and energy status in skeletal muscle［J］.Physiology (Bethesda)，2006，21：48-60.
［20］HANDSCHIN C，CHIN S，LI P，et al.Skeletal muscle fiber-type switching，exercise intolerance，and myopathy in PGC-1α muscle-specific knock-out animals［J］.J Biol Chem，2007，282(41)：30014-30021.
［21］JÄGER S，HANDSCHIN C，ST-PIERRE J，et al.AMP-activated protein kinase (AMPK) action in skeletal muscle via direct phosphorylation of PGC-1α［J］.Proc Natl Acad Sci U S A，2007，104(29)：12017-12022.
［22］RÖCKL K S，HIRSHMAN M F，BRANDAUER J，et al.Skeletal muscle adaptation to exercise training：AMP-activated protein kinase mediates muscle fiber type shift［J］.Diabetes，2007，56(8)：2062-2069.
［23］SCHIAFFINO S，SANDRI M，MURGIA M.Activity-dependent signaling pathways controlling muscle diversity and plasticity［J］.Physiology (Bethesda)，2007，22：269-278.
［24］NIKOLIC N，RHEDIN M，RUSTAN A C，et al.Overexpression of PGC-1alpha increases fatty acid oxidative capacity of human skeletal muscle cells［J］.Biochem Res Int，2012，2012：714074.
［25］MIRANDA S，FONCEA R，GUERRERO J，et al.Oxidative stress and upregulation of mitochondrial biogenesis genes in mitochondrial DNA-depleted HeLa cells［J］.Biochem Biophys Res Commun，1999，258(1)：44-49.
［26］MITSUMOTO A，TAKEUCHI A，OKAWA K，et al.A subset of newly synthesized polypeptides in mitochondria from human endothelial cells exposed to hydroperoxide stress［J］.Free Radic Biol Med，2002，32(1)：22-37.
［27］ST-PIERRE J，DRORI S，ULDRY M，et al.Suppression of reactive oxygen species and neurodegeneration by the PGC-1 transcriptional coactivators［J］.Cell，2006，127(2)：397-408.

表没食子儿茶素没食子酸酯对育肥猪骨骼肌纤维类型的影响

Effects of EGCG on Muscle Fiber Types of Finishing Pigs

RichHTML

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics

本文评价