绵羊小反刍兽疫临床病例的病理学观察

doi:10.11843/j.issn.0366-6964.2015.06.018

畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (6): 1011-1017.doi: 10.11843/j.issn.0366-6964.2015.06.018

绵羊小反刍兽疫临床病例的病理学观察

王光祥^1，2，贾宁^1*，方梅¹，孙宝¹

（1.甘肃农业大学动物医学院，兰州 730070；2.中国农业科学院兰州兽医研究所，兰州 730046)

收稿日期:2014-09-19 出版日期:2015-06-23 发布日期:2015-06-23
通讯作者: 贾宁（1963-），男，教授，博士，主要从事兽医病理学与分子病理学研究，E-mail：1963jianing@163.com
作者简介:王光祥（1976-），男，甘肃武威人，助理研究员，硕士，主要从事病毒分子生物学及免疫学研究，E-mail：wanggx666@163.com

Studies on the Pathology of the Peste des Petits Ruminants of Sheep

WANG Guang-xiang^1，2，JIA Ning^1* ，FANG Mei¹，SUN Bao¹

(1.College of Veterinary Medicine,Gansu Agricultural University，Lanzhou 730070，China；2.Lanzhou Veterinary Research Institute，Chinese Academy of Agricultural Sciences，Lanzhou 730046，China)

Received:2014-09-19 Online:2015-06-23 Published:2015-06-23

摘要/Abstract

摘要：

旨在深入探讨绵羊小反刍兽疫的病理变化特点。对临诊疑似小反刍兽疫绵羊进行RT-PCR检测和确诊，并对6例病羊进行病理解剖学和病理组织学研究。结果表明，经N基因RT-PCR检测，扩增出350 bp 左右特异性片段，确诊该羊病为绵羊小反刍兽疫。病理解剖学观察显示，病羊鼻腔、眼睛有多量黄色稀薄或黏脓性分泌物，口腔黏膜有程度不等溃烂。肺有程度不等肺炎，肝、脾表面及切面有大量针尖至粟粒大小灰白色或黄白色病灶分布。全身淋巴结肿大呈浆液性或出血性淋巴结炎，尤以肠系膜淋巴结和支气管淋巴结为重。肠黏膜呈不规则条纹状或斑块状出血，以小肠最明显，甚至形成溃疡。病理组织学观察显示，肺、支气管、肝、脾、淋巴结均有大量多核巨细胞（合胞体细胞）增生，呈灶状聚集或散在分布，细胞质中含有圆形嗜酸性包涵体。肺呈支气管间质性肺炎，肺泡及间质有大量巨噬细胞、淋巴细胞增生浸润。肝、脾、肾等器官实质广泛变性、坏死，间质中有巨噬细胞、淋巴细胞及浆细胞增生浸润。研究证明，病羊多组织器官中大量含嗜酸性胞浆包涵体多核巨细胞（合胞体细胞）的增生和支气管间质性肺炎的形成是绵羊小反刍兽疫重要并具代表性的病理变化特点。有关脾与淋巴结中大量含嗜酸性胞浆包涵体的多核巨细胞（合胞体细胞）呈灶状聚集或散在分布在国内外尚属首次报道。

Abstract:

This experiment was conducted to explore the pathological changes of the peste des petits ruminants (PPR) of sheep.On the basis of clinical diagnosis，then the six doubtful sheep of the PPR were definite diagnosed by RT-PCR detection technology.The pathological changes were observed by macroscopy and light microscope and special staining technique.The results showed that the specific N gene fragment of 350 bp of peste des petits ruminants virus (PPRV) were amplified and the PPRV was definite diagnosed as the pathogen of the six sick sheep.The anatomopathological changes showed there were a lot of yellow mucopurulent nasal discharge in nasal cavities of the sheep and there were also many secretions around the eyes.The ulcers of oral cavity mucous membrane were frequently observed.The sheep had pneumonia of different degree and there were numerous yellowish-white lesions from pinpoint-shaped to millet-shaped on the surface of the livers and spleen.The lymphoglandulae of the sheep were obviously swelling and showed serous lymphadenitis and hemorrhagic lymphadenitis.Hemorrhagic stripes，plaques and ulcers were observed on intestinal mucosa and especially on small intestinal mucosa.The histopathological changes indicated that a great number of multiple nuclear giant cells or syncytial cells were observed in lung，bronchus，liver，spleen and lymphoglandulae.The multiple nuclear giant cells or syncytial cells showed decentralized and concentrated distribution in the tissues and organs and there were eosinophil viral inclusions in their cytoplasm.Lung lesions of bronchointerstitial pneumonia were seen obviously in the sheep.There were numerous macrophages，lymphocytes in alveoli and interstitial tissue of the lungs.The parenchymal cells of the liver，kidney and spleen showed widely degeneration and necrosis.A great number of macrophages，plasma cells and lymphocytes were seen in the interstitial tissue of the liver，kidney and spleen.Those results confirmed the proliferation of multiple nuclear giant cells or syncytial cells and bronchointerstitial pneumonia were importantly histopathological changes of the peste des petits ruminants of sheep.

中图分类号:

S858.265.3

王光祥，贾宁，方梅，孙宝. 绵羊小反刍兽疫临床病例的病理学观察[J]. 畜牧兽医学报, 2015, 46(6): 1011-1017.

WANG Guang-xiang，JIA Ning，FANG Mei，SUN Bao. Studies on the Pathology of the Peste des Petits Ruminants of Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(6): 1011-1017.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.xmsyxb.com/CN/10.11843/j.issn.0366-6964.2015.06.018

https://www.xmsyxb.com/CN/Y2015/V46/I6/1011

参考文献

［1］GULYAZ V，OZKUL A.Pathogenicity of a local peste des petits ruminants virus isolate in sheep in Turkey ［J］.Trop Anim Health Prod，2005，37(7) ：541-547.
［2］TOPLU N.Characteristic and non-characteristic pathological findings in peste des petits ruminants (PPR) of sheep in the Ege District of Turkey ［J］.J Comp Pathol，2006，131(2-3)：135-141.
［3］KUMAR P，TRIPATHI B N，SHARMA A K，et al.Pathological and immunohistochemical study of experimental peste des petits ruminants virus infection in goats［J］.J Vet Med B Infect Dis Vet Public Health，2004，51(4)：153-159.
［4］KUL O，KABAKCI N，ATMACA H T，et al.Natural peste des petits ruminants virus infection：novel pathologic findings resembling other morbillivirus infections ［J］.Vet Pathol，2007，44(4)：479-486.
［5］DIALLO A，MINET C，LE GOFF C，et al.The threat of peste des petits ruminants：progress in vaccine development for disease control ［J］.Vaccine，2007，25(30)：5591-5597.
［6］ABUBAKAR M，RAJPUT Z I，ARSHED M J，et al.Evidence of peste des petits ruminants virus (PPRV) infection in Sindh Ibex (Capra aegagrus blythi) in Pakistan as confirmed by detection of antigen and antibody［J］.Trop Anim Health Prod，2011，43(4)：745-747.
［7］AL-DUBAIB M A.Peste des petits ruminants morbillivirus infection in lambs and young goats at Qassim region，Saudi Arabia［J］.Trop Anim Health Prod，2009，41(2)：217-220.
［8］OZMEN O，KALE M，HALIGUR M，et al.Pathological，serological，and virological findings in sheep infected simultaneously with Bluetongue，peste des petits ruminants，and Sheeppox viruses［J］.Trop Anim Health Prod，2009，41(6)：951-958.
［9］王乐元，次真，吴国珍，等.中国西藏小反刍兽疫的发生状况与防控［J］.畜牧兽医学报，2011，42(5)：717-720.
WANG L Y，TSE D，WU G Z，et al.Occurrence and control strategy of peste des petits ruminants in Tibet Autonomous Region of China［J］.Acta Veterinaria et Zootechnica Sinica，2011，42(5)：717-720.(in Chinese)
［10］AL-NAEEM A，ABU-ELZEIN E M E.In situ detection of PPR virus antigen in skin papules around the mouth of sheep experimentally infected with PPR virus ［J］.Trop Anim Health Prod，2008，40(4)：239-241.
［11］蒙学莲，才学鹏.小反刍兽疫病毒致病性的研究进展［J］.中国兽医科学，2010，40(7)：758-761.
MENG X L，CAI X P.Advance in pathogenicity of peste des petits ruminants virus ［J］.Chinese Veterinary Science，2010，40(7)：758-761.(in Chinese)
［12］王志亮，包静月，吴晓东，等.我国首例小反刍兽疫诊断报告［J］.中国动物检疫，2007，24(8)：24-26.
WANG Z L，BAO J Y，WU X D，et al.Diagnosis of the first outbreak of peste des petits ruminants in Tibet［J］.Chinese Journal of Animal Health Inspection，2007，24(8)：24-26.(in Chinese)
［13］龙云凤，刘晓慧，周晓黎，等.小反刍兽疫流行病学及防控研究进展［J］.动物医学进展，2012，33(5)：94-98.
LONG Y F，LIU X H，ZHOU X L，et al.Epidemiology of peste des petits ruminants and advances in prevention and control［J］. Progress in Veterinary Medicine，2012，33(5)：94-98.(in Chinese)

绵羊小反刍兽疫临床病例的病理学观察

Studies on the Pathology of the Peste des Petits Ruminants of Sheep

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

编辑推荐

Metrics

本文评价